Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   NAG05_RS09740 Genome accession   NZ_CP098025
Coordinates   1915570..1915845 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain QZ076     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1869799..1915546 1915570..1915845 flank 24


Gene organization within MGE regions


Location: 1869799..1915845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG05_RS09440 (NAG05_09440) - 1869799..1872012 (-) 2214 WP_002381746.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  NAG05_RS09445 (NAG05_09445) - 1872227..1872979 (-) 753 WP_010715313.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NAG05_RS09450 (NAG05_09450) prmA 1872981..1873928 (-) 948 WP_016622347.1 50S ribosomal protein L11 methyltransferase -
  NAG05_RS09455 (NAG05_09455) - 1873944..1874429 (-) 486 WP_010709649.1 DUF3013 family protein -
  NAG05_RS09460 (NAG05_09460) - 1874386..1875063 (-) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  NAG05_RS09465 (NAG05_09465) - 1875192..1876469 (+) 1278 WP_002360030.1 replication-associated recombination protein A -
  NAG05_RS09475 (NAG05_09475) - 1876797..1877075 (+) 279 WP_002380425.1 hypothetical protein -
  NAG05_RS09480 (NAG05_09480) - 1877253..1877726 (+) 474 WP_002357065.1 universal stress protein -
  NAG05_RS09485 (NAG05_09485) - 1877838..1879025 (-) 1188 WP_002357064.1 acetate kinase -
  NAG05_RS09490 (NAG05_09490) - 1879050..1880057 (-) 1008 WP_016622344.1 class I SAM-dependent methyltransferase -
  NAG05_RS09495 (NAG05_09495) comGG 1880186..1880539 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  NAG05_RS09500 (NAG05_09500) comGF 1880539..1880973 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  NAG05_RS09505 (NAG05_09505) - 1880963..1881289 (-) 327 WP_002393943.1 type II secretion system protein -
  NAG05_RS09510 (NAG05_09510) - 1881843..1882265 (+) 423 WP_250652826.1 hypothetical protein -
  NAG05_RS09515 (NAG05_09515) - 1882372..1883475 (-) 1104 WP_250652827.1 SH3 domain-containing protein -
  NAG05_RS09520 (NAG05_09520) - 1883517..1883723 (-) 207 WP_002395769.1 BhlA/UviB family holin-like peptide -
  NAG05_RS09525 (NAG05_09525) - 1883732..1883854 (-) 123 WP_113847225.1 XkdX family protein -
  NAG05_RS09530 (NAG05_09530) - 1883856..1884275 (-) 420 WP_250652828.1 hypothetical protein -
  NAG05_RS09535 (NAG05_09535) - 1884265..1884735 (-) 471 WP_250652829.1 hypothetical protein -
  NAG05_RS09540 (NAG05_09540) - 1884735..1885022 (-) 288 WP_010783079.1 collagen-like protein -
  NAG05_RS09545 (NAG05_09545) - 1885019..1885615 (-) 597 WP_023894828.1 hypothetical protein -
  NAG05_RS09550 (NAG05_09550) - 1885608..1886522 (-) 915 WP_250652830.1 phage baseplate upper protein -
  NAG05_RS09555 (NAG05_09555) - 1886537..1889248 (-) 2712 WP_250652831.1 phage tail tip lysozyme -
  NAG05_RS09560 (NAG05_09560) - 1889245..1889958 (-) 714 WP_250652832.1 phage tail protein -
  NAG05_RS09565 (NAG05_09565) - 1889955..1892258 (-) 2304 WP_250652833.1 phage tail tape measure protein -
  NAG05_RS09570 (NAG05_09570) - 1892450..1892905 (-) 456 WP_002373982.1 hypothetical protein -
  NAG05_RS09575 (NAG05_09575) - 1892905..1893552 (-) 648 WP_002393025.1 tail protein -
  NAG05_RS09580 (NAG05_09580) - 1893549..1893911 (-) 363 WP_002373978.1 HK97-gp10 family putative phage morphogenesis protein -
  NAG05_RS09585 (NAG05_09585) - 1893901..1894239 (-) 339 WP_002380807.1 hypothetical protein -
  NAG05_RS09590 (NAG05_09590) - 1894229..1894555 (-) 327 WP_250652834.1 phage head closure protein -
  NAG05_RS09595 (NAG05_09595) - 1894533..1894805 (-) 273 WP_033779906.1 head-tail connector protein -
  NAG05_RS09600 (NAG05_09600) - 1894817..1896211 (-) 1395 WP_250652835.1 phage major capsid protein -
  NAG05_RS09605 (NAG05_09605) - 1896224..1896796 (-) 573 WP_010826702.1 HK97 family phage prohead protease -
  NAG05_RS09610 (NAG05_09610) - 1896768..1897994 (-) 1227 WP_010826703.1 phage portal protein -
  NAG05_RS09615 (NAG05_09615) - 1898013..1899740 (-) 1728 WP_033779914.1 terminase TerL endonuclease subunit -
  NAG05_RS09620 (NAG05_09620) - 1899737..1900189 (-) 453 WP_033779917.1 P27 family phage terminase small subunit -
  NAG05_RS09625 (NAG05_09625) - 1900303..1900671 (-) 369 WP_174098580.1 HNH endonuclease signature motif containing protein -
  NAG05_RS09630 (NAG05_09630) - 1900671..1901072 (-) 402 WP_231450479.1 hypothetical protein -
  NAG05_RS09635 (NAG05_09635) - 1901205..1901621 (-) 417 WP_010826705.1 sigma-70 family RNA polymerase sigma factor -
  NAG05_RS09640 (NAG05_09640) - 1901926..1903779 (-) 1854 WP_250652836.1 phage/plasmid primase, P4 family -
  NAG05_RS09645 (NAG05_09645) - 1903854..1904606 (+) 753 WP_050588837.1 DUF4145 domain-containing protein -
  NAG05_RS09650 (NAG05_09650) - 1904603..1906165 (-) 1563 WP_033779928.1 hypothetical protein -
  NAG05_RS09655 (NAG05_09655) - 1906188..1906700 (-) 513 WP_250652837.1 hypothetical protein -
  NAG05_RS09660 (NAG05_09660) - 1906751..1907671 (-) 921 WP_250652838.1 AAA family ATPase -
  NAG05_RS09665 (NAG05_09665) - 1907668..1907886 (-) 219 WP_016626784.1 hypothetical protein -
  NAG05_RS09670 (NAG05_09670) - 1907888..1908799 (-) 912 WP_250652839.1 YqaJ viral recombinase family protein -
  NAG05_RS09675 (NAG05_09675) - 1908801..1909052 (-) 252 WP_320339136.1 hypothetical protein -
  NAG05_RS09680 (NAG05_09680) - 1909065..1910261 (-) 1197 WP_250652956.1 DEAD/DEAH box helicase -
  NAG05_RS09685 (NAG05_09685) - 1910251..1910544 (-) 294 WP_002407629.1 recombinase RecB -
  NAG05_RS09690 (NAG05_09690) - 1910541..1910741 (-) 201 WP_202577733.1 hypothetical protein -
  NAG05_RS09700 (NAG05_09700) - 1910953..1911348 (-) 396 WP_231450469.1 hypothetical protein -
  NAG05_RS09705 (NAG05_09705) - 1911591..1911902 (-) 312 WP_250652840.1 hypothetical protein -
  NAG05_RS09710 (NAG05_09710) - 1911913..1912089 (-) 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
  NAG05_RS09715 (NAG05_09715) - 1912401..1912733 (+) 333 WP_002364355.1 helix-turn-helix transcriptional regulator -
  NAG05_RS09720 (NAG05_09720) - 1912751..1913095 (+) 345 WP_002395798.1 ImmA/IrrE family metallo-endopeptidase -
  NAG05_RS09725 (NAG05_09725) - 1913129..1913857 (+) 729 WP_126262607.1 potassium channel family protein -
  NAG05_RS09730 (NAG05_09730) - 1913954..1915102 (+) 1149 WP_126262606.1 site-specific integrase -
  NAG05_RS09735 (NAG05_09735) comGD 1915130..1915573 (-) 444 WP_125203765.1 competence type IV pilus minor pilin ComGD -
  NAG05_RS09740 (NAG05_09740) comGC/cglC 1915570..1915845 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=693623 NAG05_RS09740 WP_002356991.1 1915570..1915845(-) (comGC/cglC) [Enterococcus faecalis strain QZ076]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=693623 NAG05_RS09740 WP_002356991.1 1915570..1915845(-) (comGC/cglC) [Enterococcus faecalis strain QZ076]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396