Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CFK21_RS12930 Genome accession   NZ_AP018052
Coordinates   2808211..2809431 (+) Length   406 a.a.
NCBI ID   WP_096367047.1    Uniprot ID   -
Organism   Thiohalobacter thiocyanaticus strain FOKN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2803211..2814431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK21_RS12910 - 2803221..2804102 (-) 882 WP_096367043.1 lysylphosphatidylglycerol synthase domain-containing protein -
  CFK21_RS12915 - 2804153..2805073 (-) 921 WP_096367044.1 sulfotransferase -
  CFK21_RS12920 (FOKN1_2647) - 2805225..2806133 (-) 909 WP_096367045.1 glycosyltransferase family 2 protein -
  CFK21_RS12925 (FOKN1_2648) pilB 2806395..2808116 (+) 1722 WP_096367046.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFK21_RS12930 (FOKN1_2649) pilC 2808211..2809431 (+) 1221 WP_096367047.1 type II secretion system F family protein Machinery gene
  CFK21_RS12935 (FOKN1_2650) pilD 2809442..2810317 (+) 876 WP_096367048.1 A24 family peptidase Machinery gene
  CFK21_RS12940 (FOKN1_2651) coaE 2810538..2811155 (+) 618 WP_096367049.1 dephospho-CoA kinase -
  CFK21_RS12945 (FOKN1_2652) zapD 2811475..2812245 (+) 771 WP_096367050.1 cell division protein ZapD -
  CFK21_RS12950 (FOKN1_2653) yacG 2812302..2812502 (+) 201 WP_096367051.1 DNA gyrase inhibitor YacG -
  CFK21_RS12955 (FOKN1_2654) - 2812664..2813155 (-) 492 WP_096367052.1 hypothetical protein -
  CFK21_RS12960 (FOKN1_2655) - 2813152..2814120 (-) 969 WP_096367053.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44339.14 Da        Isoelectric Point: 9.3120

>NTDB_id=68845 CFK21_RS12930 WP_096367047.1 2808211..2809431(+) (pilC) [Thiohalobacter thiocyanaticus strain FOKN1]
MAEKALKQDMFVWEGTDRRGNKVKGQTRANNQNLIKADLRRQGITPLKVKKKSALASGRRKKKIEPKDIAIFTRQLATMM
SAGVPLVQAFDIIGRGHENPSMQELVMTIKTDVEGGSNLRDALAKHPLYFDELVCNLVEAGEAAGVLDTLLDKIATYKEK
TEALKSKIKKALFYPTAVVVVAFIVTAILLIFVVPQFEELFAGFGADLPAFTRMVIDLSEFMQSYWWAVFGIIGGLIYGF
MQAKRRSPAFNHTLDKIVLKLPIVGDILTKAAIARYARTLSTMFAAGVPLVEALDSVAGATGNSVYSNAVLRMRDQVSTG
LQLQLAMNQTGLFPNMVVQMVAIGEEAGSLDAMLSKVADFYEQEVDDAVDGLSSLLEPLIMAILGVLVGGLVIAMYLPIF
KMGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=68845 CFK21_RS12930 WP_096367047.1 2808211..2809431(+) (pilC) [Thiohalobacter thiocyanaticus strain FOKN1]
ATGGCAGAGAAAGCGCTCAAACAGGATATGTTCGTCTGGGAAGGCACGGACCGGCGTGGCAACAAGGTCAAGGGCCAGAC
CCGCGCCAATAACCAGAACCTGATCAAGGCCGACCTGCGCCGTCAGGGCATCACGCCGCTCAAGGTGAAGAAGAAATCCG
CCCTGGCCTCGGGCCGCAGAAAGAAAAAGATCGAGCCCAAGGACATCGCCATCTTCACCCGCCAGCTGGCCACCATGATG
TCGGCCGGCGTACCCCTGGTGCAGGCCTTCGACATCATCGGCCGCGGTCATGAGAACCCCTCCATGCAGGAGCTGGTCAT
GACCATCAAGACCGACGTCGAGGGCGGCAGCAACCTGCGCGACGCCCTGGCCAAGCACCCGCTGTATTTCGACGAGCTGG
TGTGCAACCTGGTCGAGGCCGGCGAGGCCGCAGGTGTGCTGGATACCCTGCTGGACAAGATCGCCACCTACAAGGAAAAG
ACCGAGGCGCTGAAGAGCAAGATCAAGAAGGCCCTGTTCTATCCCACCGCCGTGGTGGTGGTGGCCTTCATCGTCACCGC
CATCCTGCTGATCTTCGTGGTCCCCCAGTTCGAGGAACTGTTCGCCGGCTTCGGCGCCGATCTGCCGGCCTTCACCCGCA
TGGTGATCGACCTGTCCGAGTTCATGCAGTCCTACTGGTGGGCGGTATTCGGCATCATCGGCGGCCTGATCTATGGTTTC
ATGCAGGCCAAGCGACGCTCGCCCGCCTTCAACCACACACTGGACAAGATCGTACTCAAGCTGCCCATTGTCGGCGACAT
CCTGACCAAGGCCGCCATCGCCCGTTATGCACGCACCCTGTCCACCATGTTCGCCGCCGGCGTGCCTCTGGTCGAGGCGC
TGGACTCGGTGGCGGGTGCGACCGGCAACAGCGTCTATTCCAATGCCGTGCTGCGCATGCGCGACCAGGTCTCCACCGGT
CTGCAGCTGCAGCTGGCAATGAACCAGACCGGCCTGTTCCCCAATATGGTGGTGCAGATGGTCGCCATCGGCGAGGAGGC
CGGCTCGCTGGACGCCATGTTGAGCAAGGTCGCCGACTTCTACGAGCAGGAGGTCGACGACGCCGTCGACGGCCTGAGCA
GCCTGCTGGAGCCGTTGATCATGGCGATCCTGGGCGTGCTGGTGGGCGGCCTGGTCATCGCCATGTATCTGCCGATCTTC
AAGATGGGCCAGGTCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.33

100

0.613
  pilC Legionella pneumophila strain ERS1305867

61.364

97.537

0.599
  pilC Acinetobacter baumannii D1279779

58.765

99.754

0.586
  pilC Acinetobacter baylyi ADP1

58.374

100

0.584
  pilG Neisseria gonorrhoeae MS11

49.132

99.261

0.488
  pilG Neisseria meningitidis 44/76-A

49.132

99.261

0.488
  pilC Vibrio cholerae strain A1552

42.222

99.754

0.421
  pilC Vibrio campbellii strain DS40M4

41.709

98.03

0.409
  pilC Thermus thermophilus HB27

38.795

100

0.397

Multiple sequence alignment