Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFK21_RS12925 Genome accession   NZ_AP018052
Coordinates   2806395..2808116 (+) Length   573 a.a.
NCBI ID   WP_096367046.1    Uniprot ID   -
Organism   Thiohalobacter thiocyanaticus strain FOKN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2801395..2813116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK21_RS12910 - 2803221..2804102 (-) 882 WP_096367043.1 lysylphosphatidylglycerol synthase domain-containing protein -
  CFK21_RS12915 - 2804153..2805073 (-) 921 WP_096367044.1 sulfotransferase -
  CFK21_RS12920 (FOKN1_2647) - 2805225..2806133 (-) 909 WP_096367045.1 glycosyltransferase family 2 protein -
  CFK21_RS12925 (FOKN1_2648) pilB 2806395..2808116 (+) 1722 WP_096367046.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFK21_RS12930 (FOKN1_2649) pilC 2808211..2809431 (+) 1221 WP_096367047.1 type II secretion system F family protein Machinery gene
  CFK21_RS12935 (FOKN1_2650) pilD 2809442..2810317 (+) 876 WP_096367048.1 A24 family peptidase Machinery gene
  CFK21_RS12940 (FOKN1_2651) coaE 2810538..2811155 (+) 618 WP_096367049.1 dephospho-CoA kinase -
  CFK21_RS12945 (FOKN1_2652) zapD 2811475..2812245 (+) 771 WP_096367050.1 cell division protein ZapD -
  CFK21_RS12950 (FOKN1_2653) yacG 2812302..2812502 (+) 201 WP_096367051.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63377.76 Da        Isoelectric Point: 4.7786

>NTDB_id=68844 CFK21_RS12925 WP_096367046.1 2806395..2808116(+) (pilB) [Thiohalobacter thiocyanaticus strain FOKN1]
MVSPNPKIQLSGLARRLVLDEIISEPDMLQALETANRDKQPLVKYLVGNKICTSRQVANAACTEFGVPLFDLSVMDMETA
PLKLVDEKLVRQHNALPLFKRGNRLFVAVSDPTNLAALDEIKFHTGITTEPILVEEDKLTKVLDSAMDAMDSTMSDLMDD
DLDNLDIQSGDEDDAGQDVDSSEIDDTPVVRFVNKILLDAINKGASDIHIEPYEKTFRVRIRQDGVLHEVANPPVNLANK
IAARVKVMSRMDIAERRVPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKIVLRILDPSSAKLGIDALGYEEHQKQLYMKNL
NKPYGMILVTGPTGSGKTVSLYTGLNILNTPDRNISTCEDPVEINLPGINQCNMNPKTGFTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVMSTLHTNDAPQTLARLMNMGVPPYNIASAVNLIIAQRLARRLCSNCKQIDDVPEAALLE
EGFKQEDVSGLKLYKANPEGCDQCTGGYKGRVGIYQVMEISEEMSRIIMDGGNALQLADQAKKEGIMDLRECGLEKVRQG
ITGLEEVNRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=68844 CFK21_RS12925 WP_096367046.1 2806395..2808116(+) (pilB) [Thiohalobacter thiocyanaticus strain FOKN1]
ATGGTATCACCGAACCCCAAAATCCAGCTCAGCGGCCTGGCGCGCCGTCTGGTACTGGACGAAATCATCTCCGAACCCGA
CATGCTCCAGGCCCTGGAGACGGCCAACCGCGACAAGCAGCCACTGGTCAAGTATCTGGTCGGCAACAAGATCTGCACCA
GCCGGCAGGTGGCCAATGCCGCCTGCACCGAGTTCGGCGTGCCGCTGTTCGACCTGTCGGTCATGGACATGGAGACGGCC
CCGCTCAAGCTGGTGGACGAGAAACTGGTCCGTCAGCACAACGCCCTGCCCCTGTTCAAGCGCGGCAACCGGCTGTTCGT
GGCTGTCTCCGACCCCACCAACCTGGCCGCCCTGGACGAGATCAAGTTCCATACCGGCATCACCACCGAACCCATCCTGG
TCGAGGAGGACAAACTCACCAAGGTCCTGGACTCGGCCATGGATGCGATGGACAGCACTATGTCCGATCTCATGGACGAC
GATCTGGACAATCTGGATATCCAGTCAGGCGACGAAGATGACGCCGGCCAGGACGTCGATTCCTCCGAAATCGACGACAC
CCCGGTGGTGCGCTTCGTCAACAAGATCCTGCTCGACGCCATCAACAAGGGGGCCTCGGACATCCACATCGAGCCCTACG
AGAAGACCTTCCGGGTGCGCATCCGCCAGGACGGTGTGCTGCACGAAGTGGCCAATCCCCCGGTAAACCTGGCCAACAAG
ATCGCCGCCCGCGTCAAGGTCATGAGCCGCATGGACATCGCCGAGCGCCGCGTCCCCCAGGACGGGCGCATCAAGATGCA
GCTCTCCAAGAACCGCGCCATCGACTTTCGTGTGAATACCTGTCCGACCCTGTTCGGCGAGAAGATCGTACTGCGTATCC
TCGACCCCAGCAGCGCCAAACTGGGCATCGACGCCCTGGGCTACGAGGAACACCAGAAACAACTCTACATGAAGAACCTG
AACAAGCCCTACGGTATGATCCTGGTCACCGGACCGACCGGTTCGGGTAAGACGGTCTCGCTCTACACCGGCCTGAATAT
CCTCAACACACCGGATCGCAACATCTCCACCTGCGAGGACCCGGTGGAAATCAATCTGCCGGGCATCAACCAGTGCAACA
TGAACCCCAAGACCGGGTTCACCTTCGCCGAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGT
GAGATCCGTGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTCATGTCCACCCTACACAC
CAACGATGCGCCCCAGACCCTGGCCCGCCTGATGAACATGGGGGTGCCGCCCTACAATATCGCCTCGGCCGTGAACCTGA
TCATCGCGCAGCGCCTGGCCCGGCGCCTGTGCAGCAACTGCAAGCAGATCGATGATGTACCCGAGGCCGCCCTGCTGGAA
GAGGGCTTCAAGCAGGAGGACGTCTCGGGGCTGAAGCTCTACAAGGCCAATCCCGAAGGTTGTGACCAGTGCACGGGCGG
CTACAAGGGCCGGGTTGGTATATATCAGGTCATGGAGATCTCCGAGGAGATGTCCCGCATCATCATGGACGGCGGCAACG
CCCTCCAGCTCGCCGATCAGGCGAAGAAAGAGGGCATCATGGACCTGCGCGAGTGCGGGCTGGAGAAGGTCAGGCAGGGC
ATCACCGGCCTGGAAGAGGTCAACCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.803

99.127

0.583
  pilB Acinetobacter baylyi ADP1

57.522

98.604

0.567
  pilB Legionella pneumophila strain ERS1305867

55.786

100

0.564
  pilB Vibrio cholerae strain A1552

54.403

89.18

0.485
  pilF Neisseria gonorrhoeae MS11

53.592

89.878

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.229

92.321

0.473
  pilB Vibrio campbellii strain DS40M4

50.095

92.321

0.462
  pilF Thermus thermophilus HB27

38.947

99.476

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

97.906

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

39.64

96.859

0.384

Multiple sequence alignment