Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   M3Y14_RS10530 Genome accession   NZ_CP097257
Coordinates   2022933..2023277 (+) Length   114 a.a.
NCBI ID   WP_016094152.1    Uniprot ID   R8DGK2
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 2017933..2028277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS10505 (M3Y14_10460) - 2018144..2018962 (-) 819 WP_264540694.1 hypothetical protein -
  M3Y14_RS10510 (M3Y14_10465) - 2019072..2019719 (+) 648 WP_085310812.1 HD domain-containing protein -
  M3Y14_RS10515 (M3Y14_10470) - 2019716..2021611 (+) 1896 WP_264540695.1 ABC-F family ATP-binding cassette domain-containing protein -
  M3Y14_RS10520 (M3Y14_10475) - 2021687..2022418 (+) 732 WP_264540696.1 Bax inhibitor-1/YccA family protein -
  M3Y14_RS10525 (M3Y14_10480) - 2022516..2022771 (+) 256 Protein_2073 DUF4318 domain-containing protein -
  M3Y14_RS10530 (M3Y14_10485) ssbA 2022933..2023277 (+) 345 WP_016094152.1 single-stranded DNA-binding protein Machinery gene
  M3Y14_RS10535 (M3Y14_10490) - 2023320..2023910 (-) 591 WP_264540697.1 TetR/AcrR family transcriptional regulator -
  M3Y14_RS10540 (M3Y14_10495) - 2023987..2024427 (-) 441 WP_264540698.1 hypothetical protein -
  M3Y14_RS10545 (M3Y14_10500) - 2024459..2025592 (-) 1134 WP_264540699.1 FAD-dependent monooxygenase -
  M3Y14_RS10550 (M3Y14_10505) - 2025852..2026979 (+) 1128 WP_219918222.1 conserved virulence factor C family protein -
  M3Y14_RS10555 (M3Y14_10510) - 2026983..2027366 (+) 384 WP_219918223.1 thiol-disulfide oxidoreductase DCC family protein -
  M3Y14_RS10560 (M3Y14_10515) - 2027449..2027883 (+) 435 WP_219918226.1 BrxA/BrxB family bacilliredoxin -

Sequence

Protein


Download         Length: 114 a.a.        Molecular weight: 13158.11 Da        Isoelectric Point: 9.9521

>NTDB_id=688155 M3Y14_RS10530 WP_016094152.1 2022933..2023277(+) (ssbA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MMNRVVLIGRLTKEPELYFTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRRSAENVTEYCTKGSLVGITGRIHTN
NYDDEKGKKIYRTEVVIESITFLERRREGASHKK

Nucleotide


Download         Length: 345 bp        

>NTDB_id=688155 M3Y14_RS10530 WP_016094152.1 2022933..2023277(+) (ssbA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTTCACAAAACAAGGTGTCGCTTATGC
GCGAATATGTGTTGCGGTGAATAGAGGGTTTCGAAATAGTTTAGGTGAACAACAAGTGGATTTTATAAATTGTGTTGTTT
GGAGAAGGTCGGCTGAAAATGTAACGGAATATTGTACGAAGGGGTCACTTGTTGGGATTACAGGGCGTATTCATACGAAT
AATTATGATGATGAGAAAGGAAAGAAAATATATAGAACTGAAGTTGTGATTGAGAGTATTACCTTTTTGGAGAGAAGGAG
GGAGGGGGCATCGCATAAGAAATAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8DGK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

54.717

92.982

0.509
  ssbB Bacillus subtilis subsp. subtilis str. 168

48.214

98.246

0.474
  ssb Latilactobacillus sakei subsp. sakei 23K

50

92.982

0.465
  ssbB Lactococcus lactis subsp. cremoris KW2

44.762

92.105

0.412
  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.727

96.491

0.412
  ssbB/cilA Streptococcus pneumoniae TIGR4

41.818

96.491

0.404
  ssbA Streptococcus mutans UA159

40.909

96.491

0.395
  ssbB/cilA Streptococcus pneumoniae Rx1

40.909

96.491

0.395
  ssbB/cilA Streptococcus pneumoniae D39

40.909

96.491

0.395
  ssbB/cilA Streptococcus pneumoniae R6

40.909

96.491

0.395
  ssbB/cilA Streptococcus mitis SK321

40.909

96.491

0.395
  ssbB/cilA Streptococcus mitis NCTC 12261

40

96.491

0.386