Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   M2912_RS08150 Genome accession   NZ_CP097069
Coordinates   1657920..1658195 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain AT40a     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1621833..1657896 1657920..1658195 flank 24


Gene organization within MGE regions


Location: 1621833..1658195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2912_RS07910 (M2912_07910) - 1621833..1622840 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  M2912_RS07915 (M2912_07915) comGG 1622969..1623322 (-) 354 WP_010710571.1 competence type IV pilus minor pilin ComGG -
  M2912_RS07920 (M2912_07920) comGF 1623322..1623756 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  M2912_RS07925 (M2912_07925) - 1623746..1624117 (-) 372 WP_172351944.1 type II secretion system protein -
  M2912_RS07930 (M2912_07930) - 1624268..1625353 (-) 1086 WP_105194863.1 SH3 domain-containing protein -
  M2912_RS07935 (M2912_07935) - 1625395..1625601 (-) 207 WP_002395769.1 BhlA/UviB family holin-like peptide -
  M2912_RS07940 (M2912_07940) - 1625627..1626844 (-) 1218 WP_129992381.1 glycerophosphodiester phosphodiesterase -
  M2912_RS07945 (M2912_07945) - 1626844..1627131 (-) 288 WP_129992380.1 collagen-like protein -
  M2912_RS07950 (M2912_07950) - 1627128..1627724 (-) 597 WP_129992379.1 hypothetical protein -
  M2912_RS07955 (M2912_07955) - 1627717..1628631 (-) 915 WP_129992378.1 phage baseplate upper protein -
  M2912_RS07960 (M2912_07960) - 1628650..1631487 (-) 2838 WP_248868060.1 phage tail tip lysozyme -
  M2912_RS07965 (M2912_07965) - 1631484..1632188 (-) 705 WP_010826698.1 hypothetical protein -
  M2912_RS07970 (M2912_07970) - 1632191..1634488 (-) 2298 WP_129992407.1 phage tail tape measure protein -
  M2912_RS07975 (M2912_07975) - 1634680..1635135 (-) 456 WP_002395308.1 hypothetical protein -
  M2912_RS07980 (M2912_07980) - 1635135..1635782 (-) 648 WP_105194772.1 phage tail protein -
  M2912_RS07985 (M2912_07985) - 1635779..1636141 (-) 363 WP_105194771.1 HK97-gp10 family putative phage morphogenesis protein -
  M2912_RS07990 (M2912_07990) - 1636131..1636469 (-) 339 WP_002398474.1 hypothetical protein -
  M2912_RS07995 (M2912_07995) - 1636459..1636785 (-) 327 WP_002373974.1 phage head closure protein -
  M2912_RS08000 (M2912_08000) - 1636763..1637044 (-) 282 WP_002395304.1 phage gp6-like head-tail connector protein -
  M2912_RS08005 (M2912_08005) - 1637047..1638408 (-) 1362 WP_105194770.1 phage major capsid protein -
  M2912_RS08010 (M2912_08010) - 1638420..1638992 (-) 573 WP_002393031.1 HK97 family phage prohead protease -
  M2912_RS08015 (M2912_08015) - 1638979..1640190 (-) 1212 WP_029736326.1 phage portal protein -
  M2912_RS08020 (M2912_08020) - 1640209..1641936 (-) 1728 WP_105194769.1 terminase TerL endonuclease subunit -
  M2912_RS08025 (M2912_08025) - 1641933..1642385 (-) 453 WP_064687382.1 P27 family phage terminase small subunit -
  M2912_RS08030 (M2912_08030) - 1642498..1642866 (-) 369 WP_002393035.1 HNH endonuclease signature motif containing protein -
  M2912_RS08035 (M2912_08035) - 1642866..1643267 (-) 402 WP_104874806.1 hypothetical protein -
  M2912_RS08040 (M2912_08040) - 1643399..1643815 (-) 417 WP_010826705.1 sigma-70 family RNA polymerase sigma factor -
  M2912_RS08045 (M2912_08045) - 1644121..1645974 (-) 1854 WP_105194767.1 phage/plasmid primase, P4 family -
  M2912_RS08050 (M2912_08050) - 1646050..1646802 (+) 753 WP_162301521.1 DUF4145 domain-containing protein -
  M2912_RS08055 (M2912_08055) - 1646799..1648361 (-) 1563 WP_105194765.1 hypothetical protein -
  M2912_RS08060 (M2912_08060) - 1648384..1648896 (-) 513 WP_016626786.1 hypothetical protein -
  M2912_RS08065 (M2912_08065) - 1648947..1649867 (-) 921 WP_105194764.1 AAA family ATPase -
  M2912_RS08070 (M2912_08070) - 1649870..1650070 (-) 201 WP_057086713.1 hypothetical protein -
  M2912_RS08075 (M2912_08075) - 1650067..1650210 (-) 144 WP_226088781.1 hypothetical protein -
  M2912_RS08080 (M2912_08080) - 1650279..1651190 (-) 912 WP_105194763.1 YqaJ viral recombinase family protein -
  M2912_RS08085 (M2912_08085) - 1651203..1651445 (-) 243 WP_010826713.1 hypothetical protein -
  M2912_RS08090 (M2912_08090) - 1651447..1652643 (-) 1197 WP_016626781.1 DEAD/DEAH box helicase -
  M2912_RS08095 (M2912_08095) - 1652633..1652926 (-) 294 WP_010826715.1 VRR-NUC domain-containing protein -
  M2912_RS08100 (M2912_08100) - 1652923..1653123 (-) 201 WP_105194762.1 hypothetical protein -
  M2912_RS08105 (M2912_08105) - 1653338..1653754 (-) 417 WP_226088780.1 hypothetical protein -
  M2912_RS08110 (M2912_08110) - 1653755..1653934 (-) 180 WP_002395800.1 hypothetical protein -
  M2912_RS08115 (M2912_08115) - 1653941..1654252 (-) 312 WP_105194760.1 hypothetical protein -
  M2912_RS08120 (M2912_08120) - 1654263..1654439 (-) 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
  M2912_RS08125 (M2912_08125) - 1654751..1655083 (+) 333 WP_002364355.1 helix-turn-helix transcriptional regulator -
  M2912_RS08130 (M2912_08130) - 1655101..1655445 (+) 345 WP_105194759.1 ImmA/IrrE family metallo-endopeptidase -
  M2912_RS08135 (M2912_08135) - 1655479..1656207 (+) 729 WP_033918699.1 potassium channel family protein -
  M2912_RS08140 (M2912_08140) - 1656304..1657452 (+) 1149 WP_105194837.1 site-specific integrase -
  M2912_RS08145 (M2912_08145) comGD 1657480..1657923 (-) 444 WP_129992406.1 competence type IV pilus minor pilin ComGD -
  M2912_RS08150 (M2912_08150) comGC/cglC 1657920..1658195 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=686903 M2912_RS08150 WP_002356991.1 1657920..1658195(-) (comGC/cglC) [Enterococcus faecalis strain AT40a]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=686903 M2912_RS08150 WP_002356991.1 1657920..1658195(-) (comGC/cglC) [Enterococcus faecalis strain AT40a]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396