Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   M2913_RS08345 Genome accession   NZ_CP097034
Coordinates   1683889..1684164 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain AT40b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1641769..1683865 1683889..1684164 flank 24


Gene organization within MGE regions


Location: 1641769..1684164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2913_RS08035 (M2913_08035) - 1641769..1642776 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  M2913_RS08040 (M2913_08040) comGG 1642905..1643258 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  M2913_RS08045 (M2913_08045) comGF 1643258..1643692 (-) 435 WP_002378441.1 competence type IV pilus minor pilin ComGF -
  M2913_RS08050 (M2913_08050) - 1643682..1644008 (-) 327 WP_002370967.1 type II secretion system protein -
  M2913_RS08055 (M2913_08055) hemH 1644811..1645752 (-) 942 WP_002357056.1 ferrochelatase -
  M2913_RS08065 (M2913_08065) - 1646456..1647715 (-) 1260 WP_248878661.1 LysM peptidoglycan-binding domain-containing protein -
  M2913_RS08070 (M2913_08070) - 1647720..1647923 (-) 204 WP_010711247.1 phage holin -
  M2913_RS08075 (M2913_08075) - 1647920..1648141 (-) 222 WP_002397443.1 hypothetical protein -
  M2913_RS08080 (M2913_08080) - 1648169..1648324 (-) 156 WP_002364192.1 XkdX family protein -
  M2913_RS08085 (M2913_08085) - 1648326..1648646 (-) 321 WP_010711695.1 hypothetical protein -
  M2913_RS08090 (M2913_08090) - 1648660..1649151 (-) 492 WP_010711694.1 hypothetical protein -
  M2913_RS08095 (M2913_08095) - 1649151..1649438 (-) 288 WP_151152345.1 collagen-like protein -
  M2913_RS08100 (M2913_08100) - 1649435..1650031 (-) 597 WP_248878663.1 hypothetical protein -
  M2913_RS08105 (M2913_08105) - 1650024..1650905 (-) 882 WP_010711691.1 phage baseplate upper protein -
  M2913_RS08110 (M2913_08110) - 1650924..1653731 (-) 2808 WP_248878665.1 phage tail spike protein -
  M2913_RS08115 (M2913_08115) - 1653713..1654447 (-) 735 WP_002357042.1 hypothetical protein -
  M2913_RS08120 (M2913_08120) - 1654437..1657334 (-) 2898 WP_248878783.1 tape measure protein -
  M2913_RS08125 (M2913_08125) - 1657582..1657932 (-) 351 WP_002397453.1 hypothetical protein -
  M2913_RS08130 (M2913_08130) - 1657985..1658833 (-) 849 WP_159162313.1 major tail protein -
  M2913_RS08135 (M2913_08135) - 1658834..1659208 (-) 375 WP_002357037.1 DUF6838 family protein -
  M2913_RS08140 (M2913_08140) - 1659211..1659609 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  M2913_RS08145 (M2913_08145) - 1659602..1659970 (-) 369 WP_010711687.1 hypothetical protein -
  M2913_RS08150 (M2913_08150) - 1659967..1660311 (-) 345 WP_002387282.1 hypothetical protein -
  M2913_RS08155 (M2913_08155) - 1660325..1660507 (-) 183 WP_002387281.1 hypothetical protein -
  M2913_RS08160 (M2913_08160) - 1660536..1661423 (-) 888 WP_010707370.1 DUF5309 domain-containing protein -
  M2913_RS08165 (M2913_08165) - 1661437..1662060 (-) 624 WP_010707369.1 DUF4355 domain-containing protein -
  M2913_RS08170 (M2913_08170) - 1662279..1662599 (-) 321 WP_002380436.1 hypothetical protein -
  M2913_RS08175 (M2913_08175) - 1662658..1662903 (-) 246 WP_002397460.1 hypothetical protein -
  M2913_RS08180 (M2913_08180) - 1662908..1664605 (-) 1698 WP_248878668.1 toxin glutamine deamidase domain-containing protein -
  M2913_RS08185 (M2913_08185) - 1664580..1666067 (-) 1488 WP_248878670.1 phage portal protein -
  M2913_RS08190 (M2913_08190) - 1666079..1667368 (-) 1290 WP_104882475.1 PBSX family phage terminase large subunit -
  M2913_RS08195 (M2913_08195) terS 1667340..1668134 (-) 795 WP_070416179.1 phage terminase small subunit -
  M2913_RS08200 (M2913_08200) - 1668187..1668411 (-) 225 WP_002404955.1 hypothetical protein -
  M2913_RS08210 (M2913_08210) - 1669456..1669593 (+) 138 WP_010773976.1 hypothetical protein -
  M2913_RS08215 (M2913_08215) - 1669629..1669892 (-) 264 WP_174084463.1 hypothetical protein -
  M2913_RS08220 (M2913_08220) - 1670245..1670712 (-) 468 WP_104860095.1 ArpU family phage packaging/lysis transcriptional regulator -
  M2913_RS08225 (M2913_08225) - 1671072..1671278 (-) 207 WP_116491842.1 hypothetical protein -
  M2913_RS08230 (M2913_08230) - 1671292..1671876 (-) 585 WP_248878672.1 DUF3850 domain-containing protein -
  M2913_RS08235 (M2913_08235) - 1671888..1672136 (-) 249 WP_002415426.1 hypothetical protein -
  M2913_RS08240 (M2913_08240) - 1672133..1672486 (-) 354 WP_002415427.1 hypothetical protein -
  M2913_RS08245 (M2913_08245) - 1672636..1673331 (-) 696 WP_010711678.1 N-6 DNA methylase -
  M2913_RS08250 (M2913_08250) - 1673681..1674115 (-) 435 WP_024797132.1 RusA family crossover junction endodeoxyribonuclease -
  M2913_RS08255 (M2913_08255) - 1674124..1674423 (-) 300 WP_002372047.1 MazG-like family protein -
  M2913_RS08260 (M2913_08260) - 1674424..1674726 (-) 303 WP_002368214.1 hypothetical protein -
  M2913_RS08265 (M2913_08265) - 1674730..1675692 (-) 963 WP_002372048.1 Lin1244/Lin1753 domain-containing protein -
  M2913_RS08270 (M2913_08270) - 1675706..1676596 (-) 891 WP_002364218.1 recombinase RecT -
  M2913_RS08275 (M2913_08275) - 1676599..1677540 (-) 942 WP_002364219.1 lambda-exonuclease family protein -
  M2913_RS08280 (M2913_08280) - 1677638..1677865 (-) 228 WP_002364220.1 hypothetical protein -
  M2913_RS08285 (M2913_08285) - 1677865..1678188 (-) 324 WP_002380444.1 hypothetical protein -
  M2913_RS08290 (M2913_08290) - 1678232..1678417 (-) 186 WP_002364222.1 hypothetical protein -
  M2913_RS08295 (M2913_08295) - 1678408..1678602 (-) 195 WP_002364223.1 hypothetical protein -
  M2913_RS08300 (M2913_08300) - 1678655..1678837 (+) 183 WP_002364224.1 YegP family protein -
  M2913_RS08305 (M2913_08305) - 1678877..1679599 (-) 723 WP_002364225.1 phage regulatory protein -
  M2913_RS08310 (M2913_08310) - 1679638..1679955 (-) 318 WP_002364228.1 hypothetical protein -
  M2913_RS08315 (M2913_08315) - 1679961..1680152 (-) 192 WP_002356998.1 hypothetical protein -
  M2913_RS08320 (M2913_08320) - 1680443..1680787 (+) 345 WP_002385819.1 helix-turn-helix transcriptional regulator -
  M2913_RS08325 (M2913_08325) - 1680792..1681442 (+) 651 WP_025191954.1 ImmA/IrrE family metallo-endopeptidase -
  M2913_RS08330 (M2913_08330) - 1681538..1682167 (+) 630 WP_002387265.1 SHOCT domain-containing protein -
  M2913_RS08335 (M2913_08335) - 1682273..1683421 (+) 1149 WP_010711674.1 site-specific integrase -
  M2913_RS08340 (M2913_08340) comGD 1683455..1683892 (-) 438 WP_025191953.1 competence type IV pilus minor pilin ComGD -
  M2913_RS08345 (M2913_08345) comGC/cglC 1683889..1684164 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=686446 M2913_RS08345 WP_002356991.1 1683889..1684164(-) (comGC/cglC) [Enterococcus faecalis strain AT40b]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=686446 M2913_RS08345 WP_002356991.1 1683889..1684164(-) (comGC/cglC) [Enterococcus faecalis strain AT40b]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396