Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CCX78_RS03230 Genome accession   NZ_AP017931
Coordinates   617209..618099 (+) Length   296 a.a.
NCBI ID   WP_016265378.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain LK-145     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 612209..623099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX78_RS03200 (LASAK_00633) - 612644..613714 (+) 1071 WP_148664863.1 hypothetical protein -
  CCX78_RS03205 (LASAK_00634) - 613722..615113 (+) 1392 WP_096584638.1 hypothetical protein -
  CCX78_RS03215 (LASAK_00635) - 615300..615692 (-) 393 WP_231952369.1 hypothetical protein -
  CCX78_RS03220 (LASAK_00636) - 615771..616271 (+) 501 WP_011375003.1 VanZ family protein -
  CCX78_RS03225 (LASAK_00637) - 616362..617093 (+) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CCX78_RS03230 (LASAK_00638) comGA 617209..618099 (+) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  CCX78_RS03235 (LASAK_00639) comGB 618092..619099 (+) 1008 WP_011375000.1 type II secretion system F family protein Machinery gene
  CCX78_RS03240 (LASAK_00640) comGC 619096..619395 (+) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CCX78_RS03245 (LASAK_00641) comGD 619367..619819 (+) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  CCX78_RS03250 (LASAK_00642) comGE 619806..620111 (+) 306 WP_011374997.1 hypothetical protein Machinery gene
  CCX78_RS03255 (LASAK_00643) comGF 620077..620598 (+) 522 WP_112234088.1 competence type IV pilus minor pilin ComGF Machinery gene
  CCX78_RS03260 (LASAK_00644) - 620633..620899 (+) 267 WP_172425585.1 hypothetical protein -
  CCX78_RS03265 (LASAK_00645) - 621014..622033 (+) 1020 WP_231952370.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32972.78 Da        Isoelectric Point: 6.3840

>NTDB_id=68583 CCX78_RS03230 WP_016265378.1 617209..618099(+) (comGA) [Latilactobacillus sakei strain LK-145]
MSTEEKLTTLLKHCAKHYVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQSGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYVVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGSDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=68583 CCX78_RS03230 WP_016265378.1 617209..618099(+) (comGA) [Latilactobacillus sakei strain LK-145]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAGCACTATGTGCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGTCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAGATTCGCGATTTAG
AGACAGCCCAATACGTGGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAGTTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTTCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

99.324

100

0.993
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.279

91.892

0.389
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.912

91.892

0.385
  comGA Lactococcus lactis subsp. cremoris KW2

37.793

100

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

41.241

92.568

0.382
  comYA Streptococcus gordonii str. Challis substr. CH1

40.809

91.892

0.375
  comYA Streptococcus mutans UA140

38.909

92.905

0.361
  comYA Streptococcus mutans UA159

38.909

92.905

0.361

Multiple sequence alignment