Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PALA37_RS16995 Genome accession   NZ_CP109850
Coordinates   3648804..3649268 (+) Length   154 a.a.
NCBI ID   WP_128697780.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3643804..3654268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA37_RS16970 (PALA37_03361) yacG 3643974..3644174 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA37_RS16975 (PALA37_03362) coaE 3644171..3644782 (-) 612 WP_127429831.1 dephospho-CoA kinase -
  PALA37_RS16980 (PALA37_03363) pilD 3644779..3645651 (-) 873 WP_270132262.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA37_RS16985 (PALA37_03364) pilC 3645652..3646869 (-) 1218 WP_269974491.1 type II secretion system F family protein Machinery gene
  PALA37_RS16990 (PALA37_03365) pilB 3646873..3648573 (-) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA37_RS16995 (PALA37_03366) pilA/pilAI 3648804..3649268 (+) 465 WP_128697780.1 pilin Machinery gene
  PALA37_RS17000 (PALA37_03367) - 3649401..3650729 (+) 1329 WP_270132273.1 O-antigen ligase family protein -
  PALA37_RS17010 (PALA37_03369) nadC 3650910..3651758 (-) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16137.39 Da        Isoelectric Point: 5.1594

>NTDB_id=680748 PALA37_RS16995 WP_128697780.1 3648804..3649268(+) (pilA/pilAI) [Pseudomonas aeruginosa strain PALA37]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPDDDQYDIGFTG
STLLKGSEKEQITVKNGTLPTVQLVATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPDNWKANYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=680748 PALA37_RS16995 WP_128697780.1 3648804..3649268(+) (pilA/pilAI) [Pseudomonas aeruginosa strain PALA37]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTTAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTGACGATGACCAGTATGATATCGGATTTACCGGT
TCTACCTTGTTGAAAGGATCGGAGAAAGAGCAGATTACTGTGAAAAATGGTACTCTGCCTACCGTACAGTTGGTAGCCAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTCTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGATAACTGGAAAGCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.059

99.351

0.468
  pilA Pseudomonas aeruginosa PAK

42.038

100

0.429
  pilA Acinetobacter baumannii strain A118

43.137

99.351

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

36.416

100

0.409
  pilA Vibrio cholerae strain A1552

37.5

100

0.39
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.39
  pilA Vibrio cholerae C6706

37.5

100

0.39
  comP Acinetobacter baylyi ADP1

36.585

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

42.336

88.961

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37