Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MZ018_RS19490 Genome accession   NZ_CP096163
Coordinates   4408462..4409574 (-) Length   370 a.a.
NCBI ID   WP_248065812.1    Uniprot ID   -
Organism   Shewanella sp. JNE10-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4403462..4414574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZ018_RS19455 (MZ018_19455) - 4403988..4404548 (-) 561 WP_011788682.1 DUF4136 domain-containing protein -
  MZ018_RS19460 (MZ018_19460) - 4404727..4405041 (+) 315 WP_011919670.1 hypothetical protein -
  MZ018_RS19465 (MZ018_19465) yciH 4405207..4405536 (+) 330 WP_011788680.1 stress response translation initiation inhibitor YciH -
  MZ018_RS19470 (MZ018_19470) - 4405649..4406212 (+) 564 WP_011788679.1 YqgE/AlgH family protein -
  MZ018_RS19475 (MZ018_19475) ruvX 4406244..4406669 (+) 426 WP_011788678.1 Holliday junction resolvase RuvX -
  MZ018_RS19480 (MZ018_19480) hemH 4406738..4407751 (-) 1014 WP_248065814.1 ferrochelatase -
  MZ018_RS19485 (MZ018_19485) - 4407784..4408323 (-) 540 WP_248065813.1 glutathione peroxidase -
  MZ018_RS19490 (MZ018_19490) pilU 4408462..4409574 (-) 1113 WP_248065812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MZ018_RS19495 (MZ018_19495) pilT 4409584..4410621 (-) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  MZ018_RS19500 (MZ018_19500) - 4410657..4411355 (+) 699 WP_011788673.1 YggS family pyridoxal phosphate-dependent enzyme -
  MZ018_RS19505 (MZ018_19505) proC 4411536..4412354 (+) 819 WP_248065811.1 pyrroline-5-carboxylate reductase -
  MZ018_RS19510 (MZ018_19510) - 4412401..4412949 (+) 549 WP_011788671.1 YggT family protein -
  MZ018_RS19515 (MZ018_19515) yggU 4412949..4413239 (+) 291 WP_011788670.1 DUF167 family protein YggU -
  MZ018_RS19520 (MZ018_19520) - 4413317..4413751 (+) 435 WP_011788669.1 DUF4426 domain-containing protein -
  MZ018_RS19525 (MZ018_19525) rdgB 4413862..4414479 (+) 618 WP_011919679.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41098.22 Da        Isoelectric Point: 6.0920

>NTDB_id=680155 MZ018_RS19490 WP_248065812.1 4408462..4409574(-) (pilU) [Shewanella sp. JNE10-2]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLELYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=680155 MZ018_RS19490 WP_248065812.1 4408462..4409574(-) (pilU) [Shewanella sp. JNE10-2]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGGGTCAGCGCTTTTTGGCAAAGGGAATCACCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACTGAGTCATTCGATGC
GGCATTAAAAAGCTCCCTTCGTCAGGCACCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCCAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTATTAATTAATACTCCAAGGGTAGCGAGTTTAA
TTGCCAAAAATGAGCTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAACAGGGGATGCAGACATTCGACCAAGCG
TTACTGGAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT
GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGCGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362