Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   OBG92_RS24675 Genome accession   NZ_CP109657
Coordinates   5230741..5231208 (-) Length   155 a.a.
NCBI ID   WP_034081326.1    Uniprot ID   Q8KQ32
Organism   Pseudomonas aeruginosa strain Zw26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5225741..5236208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OBG92_RS24650 (OBG92_04855) nadC 5226622..5227470 (+) 849 WP_003152758.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OBG92_RS24660 (OBG92_04857) tfpZ 5227985..5228737 (-) 753 WP_034081328.1 TfpX/TfpZ family type IV pilin accessory protein -
  OBG92_RS24665 - 5228734..5229291 (-) 558 WP_256429679.1 hypothetical protein -
  OBG92_RS24670 (OBG92_04858) - 5229297..5230676 (-) 1380 WP_264315595.1 hypothetical protein -
  OBG92_RS24675 (OBG92_04859) pilA2 5230741..5231208 (-) 468 WP_034081326.1 pilin Machinery gene
  OBG92_RS24685 (OBG92_04860) pilB 5231431..5233134 (+) 1704 WP_033998207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OBG92_RS24690 (OBG92_04861) pilC 5233138..5234355 (+) 1218 WP_034081324.1 type II secretion system F family protein Machinery gene
  OBG92_RS24695 (OBG92_04862) pilD 5234358..5235230 (+) 873 WP_264315596.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  OBG92_RS24700 (OBG92_04863) coaE 5235227..5235838 (+) 612 WP_003152767.1 dephospho-CoA kinase -
  OBG92_RS24705 (OBG92_04864) yacG 5235835..5236035 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15761.99 Da        Isoelectric Point: 8.4401

>NTDB_id=679709 OBG92_RS24675 WP_034081326.1 5230741..5231208(-) (pilA2) [Pseudomonas aeruginosa strain Zw26]
MKAQKGFTLIELMIVVAIIGILAAVAIPAYQDYITRGQVTEAVSLGGGLKSPLAEYGADKNAWPTLVAPTATPGAGQLNA
TLVGKYSSVDSTIASGYPNGQITVTMTQGKASGKKLTFSTQDGGSSWACGNASIDGFAGTGTTIDAKYLPNACKP

Nucleotide


Download         Length: 468 bp        

>NTDB_id=679709 OBG92_RS24675 WP_034081326.1 5230741..5231208(-) (pilA2) [Pseudomonas aeruginosa strain Zw26]
ATGAAAGCGCAAAAAGGCTTTACCCTGATCGAACTAATGATCGTGGTGGCGATCATCGGCATTCTGGCTGCCGTCGCTAT
CCCGGCTTATCAGGACTACATCACCCGCGGTCAGGTAACCGAGGCCGTATCCCTGGGCGGTGGTCTGAAGTCCCCGCTGG
CCGAGTATGGCGCCGACAAGAACGCTTGGCCGACTCTGGTTGCCCCAACTGCTACTCCTGGGGCTGGTCAATTGAATGCA
ACTCTGGTTGGCAAGTATTCGAGCGTAGATAGCACCATTGCCAGCGGCTATCCTAACGGTCAGATCACTGTCACCATGAC
TCAAGGTAAGGCTTCTGGTAAGAAGCTGACCTTCAGCACTCAAGACGGGGGTTCGTCTTGGGCGTGTGGTAATGCTTCTA
TCGACGGTTTTGCCGGTACCGGTACCACGATCGACGCTAAGTACCTGCCGAACGCTTGCAAGCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-154)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KQ32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.743

100

0.477
  pilA2 Legionella pneumophila str. Paris

41.875

100

0.432
  pilA2 Legionella pneumophila strain ERS1305867

41.875

100

0.432
  pilE Neisseria gonorrhoeae MS11

41.104

100

0.432
  pilE Neisseria gonorrhoeae strain FA1090

41.509

100

0.426
  pilA Ralstonia pseudosolanacearum GMI1000

38.462

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.024

100

0.413
  comP Acinetobacter baylyi ADP1

39.375

100

0.406
  pilA Pseudomonas aeruginosa PAK

36.257

100

0.4
  pilA/pilA1 Eikenella corrodens VA1

37.107

100

0.381
  pilA Vibrio parahaemolyticus RIMD 2210633

43.182

85.161

0.368