Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MRO13_RS01425 Genome accession   NZ_CP095311
Coordinates   309364..311085 (+) Length   573 a.a.
NCBI ID   WP_158144635.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304364..316085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS01405 (MRO13_01405) pilM 306655..307662 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  MRO13_RS01410 (MRO13_01410) pilN 307652..308245 (+) 594 WP_110167721.1 PilN domain-containing protein Machinery gene
  MRO13_RS01415 (MRO13_01415) pilO 308238..308828 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRO13_RS01420 (MRO13_01420) pilP 308818..309339 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  MRO13_RS01425 (MRO13_01425) pilQ 309364..311085 (+) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  MRO13_RS01430 (MRO13_01430) aroK 311272..311790 (+) 519 WP_040903956.1 shikimate kinase AroK -
  MRO13_RS01435 (MRO13_01435) aroB 311819..312904 (+) 1086 WP_004394485.1 3-dehydroquinate synthase -
  MRO13_RS01440 (MRO13_01440) - 313048..314496 (+) 1449 WP_350939998.1 AAA family ATPase -
  MRO13_RS01445 (MRO13_01445) - 314565..315380 (+) 816 WP_004394487.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62146.53 Da        Isoelectric Point: 4.8607

>NTDB_id=676276 MRO13_RS01425 WP_158144635.1 309364..311085(+) (pilQ) [Vibrio metschnikovii strain 20CO01TI02]
MIKGLPSSIAVVLRYLCITLWLGLALTTQAALASDSNQFKHIDFRVTPDKAAVIVIELASASSVVDVQKTTQGLSIELLK
TDVAEEQLYLLDVQDFATIVESVEVFRQTPSTLLLAKIHGPFHYDYQLKGRYVEVTITPLKAEVQSAPANLLEPSAKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVILVAPKAELDLREKQQL
EKARLAEELGDLQSAIIKISFAKASDIAEMIGGTGAVNMLSERGSISIDERTNALLIRELPENIHVIREIISALDIPVKQ
VQIEARIVTVNEGNLDELGIRWGVSSTRGNNTVGGSIEGNLTGIGLYQPEDGSEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLQVTPQITPDNRLVL
DLSVTQDRPGDIVKTGTGEAVAIHTQRIGTQVLVNNGETVVLGGIFQHSITNTVDKVPLLGDLPLLGALFRRSYQQMGKS
ELLIFVTPKVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=676276 MRO13_RS01425 WP_158144635.1 309364..311085(+) (pilQ) [Vibrio metschnikovii strain 20CO01TI02]
ATGATTAAAGGATTACCTTCAAGTATCGCGGTAGTGCTGCGCTATCTGTGCATCACGCTATGGCTAGGTTTAGCATTGAC
GACGCAGGCTGCTCTAGCGAGTGATAGCAATCAGTTTAAACACATCGATTTTCGGGTAACCCCCGACAAAGCCGCGGTGA
TTGTGATTGAGTTAGCGTCGGCATCGAGTGTGGTTGATGTCCAGAAAACGACGCAGGGGTTGAGTATTGAATTATTAAAA
ACCGATGTGGCTGAGGAACAGCTTTATTTACTTGATGTTCAAGATTTCGCAACCATAGTTGAGAGTGTGGAAGTTTTTCG
TCAAACACCAAGCACGTTGTTGTTGGCTAAAATACACGGTCCCTTTCATTATGATTATCAGCTCAAAGGTCGTTACGTTG
AAGTGACGATCACGCCGTTAAAAGCCGAGGTTCAAAGTGCGCCAGCCAATCTACTTGAACCATCAGCCAAGCTGATTTCG
ATTAACTTTCAAGACATTCCGGTACGTAACGTTTTACAACTGATCGCTGATTATAATCAGTTTAACTTAGTGGTATCTGA
TTCAGTGGTAGGGAATTTAACCCTGCGTTTAGATGGTGTGCCATGGCAGCAAGTGTTGGACATTATTTTGCAGGTGAAAG
GGTTGGATAAGCGGGTTGAGGGCAATGTGATTTTGGTAGCGCCTAAGGCGGAACTCGATTTACGTGAAAAACAACAGCTG
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGAGACTTGCAGTCAGCCATCATCAAAATAAGTTTCGCGAAGGCGTCAGATAT
CGCTGAGATGATTGGTGGGACGGGAGCGGTGAATATGCTCTCTGAACGAGGTTCAATCAGTATCGATGAACGTACCAATG
CCTTATTAATTCGCGAATTGCCGGAAAATATTCATGTGATCCGAGAGATCATTTCGGCGTTAGATATCCCTGTTAAGCAG
GTACAAATTGAAGCGCGAATTGTGACCGTGAATGAAGGTAATCTTGATGAACTCGGTATTCGTTGGGGCGTGAGCTCAAC
ACGCGGTAATAATACAGTTGGTGGTTCAATAGAAGGTAACTTAACGGGTATTGGTCTCTATCAACCAGAAGATGGTAGCG
AAATGATCAATAACTTCCTTAATGTCAATTTGGGAGCCACAGCAGCGAATGCTTCGAGCATCGCGTTTCAGGTGGCAAAG
CTGGGTTCAGGGACACTGCTCGATCTTGAACTGTCAGCGATGCAGCGAGAATCGAAGGCGGAAATTATTTCCAGTCCACG
GCTTATCACTACCAATAAAAAGCCAGCTTATATAGAGCAGGGGACAGAGATCCCCTATTTAGAATCGTCTTCCAGCGGTG
CGACTACGGTGGCATTTAAGAAAGCGGTACTGAGTTTACAGGTTACCCCGCAGATTACGCCGGATAATCGTTTGGTGCTG
GATTTAAGTGTTACCCAAGACCGACCTGGCGATATTGTCAAAACCGGTACCGGAGAAGCGGTAGCGATCCACACGCAACG
GATTGGTACTCAAGTTTTAGTCAATAATGGCGAAACCGTGGTACTGGGCGGTATTTTCCAACACAGCATCACTAATACGG
TCGATAAAGTGCCATTACTGGGTGATTTACCGTTACTCGGTGCGCTTTTTCGACGTAGCTATCAGCAAATGGGCAAAAGT
GAGCTACTGATTTTTGTTACTCCAAAGGTTGTAATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.95

100

0.775
  pilQ Vibrio cholerae strain A1552

76.95

100

0.775
  pilQ Vibrio campbellii strain DS40M4

75.818

95.986

0.728
  pilQ Legionella pneumophila strain Lp02

41.852

94.241

0.394
  pilQ Legionella pneumophila strain ERS1305867

41.852

94.241

0.394
  pilQ Pseudomonas aeruginosa PAK

40.468

97.033

0.393
  comQ Acinetobacter baylyi ADP1

39.076

98.255

0.384
  pilQ Acinetobacter baumannii strain A118

36.269

100

0.366
  pilQ Acinetobacter baumannii D1279779

36.269

100

0.366