Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   MRO13_RS01410 Genome accession   NZ_CP095311
Coordinates   307652..308245 (+) Length   197 a.a.
NCBI ID   WP_110167721.1    Uniprot ID   A0A317ZW17
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 302652..313245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS01395 (MRO13_01395) oxyR 303080..303979 (+) 900 WP_004394478.1 DNA-binding transcriptional regulator OxyR -
  MRO13_RS01400 (MRO13_01400) - 304033..306513 (-) 2481 WP_154169993.1 PBP1A family penicillin-binding protein -
  MRO13_RS01405 (MRO13_01405) pilM 306655..307662 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  MRO13_RS01410 (MRO13_01410) pilN 307652..308245 (+) 594 WP_110167721.1 PilN domain-containing protein Machinery gene
  MRO13_RS01415 (MRO13_01415) pilO 308238..308828 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRO13_RS01420 (MRO13_01420) pilP 308818..309339 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  MRO13_RS01425 (MRO13_01425) pilQ 309364..311085 (+) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  MRO13_RS01430 (MRO13_01430) aroK 311272..311790 (+) 519 WP_040903956.1 shikimate kinase AroK -
  MRO13_RS01435 (MRO13_01435) aroB 311819..312904 (+) 1086 WP_004394485.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22548.87 Da        Isoelectric Point: 9.9581

>NTDB_id=676273 MRO13_RS01410 WP_110167721.1 307652..308245(+) (pilN) [Vibrio metschnikovii strain 20CO01TI02]
MLHSINLFPWREAQRADHKRRFLQLMSLGVLVALLMQWGAGHYLDQQQQHQQARLDFLNQHLQQLDQRLRALKMTEAEHQ
ALQARLSVVESLQQKRNKTTQLMNLMPELIPEGVYVDKISMKGDAVEVSGISDSTARLATMLDKLERSPHLSAVGMHSIV
AGNQRFNKDFQSFKVSFHFHTAQPGLEALIRQGEHHG

Nucleotide


Download         Length: 594 bp        

>NTDB_id=676273 MRO13_RS01410 WP_110167721.1 307652..308245(+) (pilN) [Vibrio metschnikovii strain 20CO01TI02]
ATGTTGCATAGTATCAATTTATTCCCTTGGCGAGAGGCGCAGCGTGCTGATCATAAACGGCGCTTTCTCCAGCTTATGAG
TCTTGGTGTCTTAGTCGCTTTGCTCATGCAATGGGGTGCCGGCCATTACCTTGATCAGCAACAACAGCACCAACAGGCAC
GATTAGATTTTTTGAATCAGCATCTTCAGCAACTGGATCAGAGATTACGGGCGCTCAAAATGACCGAAGCTGAGCATCAA
GCACTGCAAGCCCGGCTCAGTGTTGTTGAGTCCTTACAGCAAAAGCGCAATAAAACCACACAATTGATGAACTTGATGCC
AGAGTTGATTCCTGAAGGGGTTTACGTCGACAAAATTAGTATGAAGGGGGATGCGGTCGAAGTCAGCGGGATTAGCGATA
GTACCGCACGCTTAGCCACCATGTTAGATAAATTAGAAAGGTCACCACACTTATCAGCAGTAGGCATGCACTCGATTGTC
GCTGGGAATCAGCGTTTTAATAAAGATTTTCAGAGTTTTAAGGTCTCTTTTCATTTTCATACTGCTCAACCAGGATTAGA
GGCGTTGATTCGGCAAGGTGAACATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A317ZW17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

60.406

100

0.604
  pilN Vibrio campbellii strain DS40M4

55.838

100

0.558