Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MRO13_RS01405 Genome accession   NZ_CP095311
Coordinates   306655..307662 (+) Length   335 a.a.
NCBI ID   WP_154169995.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301655..312662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS01390 (MRO13_01390) - 302557..302844 (-) 288 WP_040903944.1 DUF3624 domain-containing protein -
  MRO13_RS01395 (MRO13_01395) oxyR 303080..303979 (+) 900 WP_004394478.1 DNA-binding transcriptional regulator OxyR -
  MRO13_RS01400 (MRO13_01400) - 304033..306513 (-) 2481 WP_154169993.1 PBP1A family penicillin-binding protein -
  MRO13_RS01405 (MRO13_01405) pilM 306655..307662 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  MRO13_RS01410 (MRO13_01410) pilN 307652..308245 (+) 594 WP_110167721.1 PilN domain-containing protein Machinery gene
  MRO13_RS01415 (MRO13_01415) pilO 308238..308828 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRO13_RS01420 (MRO13_01420) pilP 308818..309339 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  MRO13_RS01425 (MRO13_01425) pilQ 309364..311085 (+) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  MRO13_RS01430 (MRO13_01430) aroK 311272..311790 (+) 519 WP_040903956.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37445.04 Da        Isoelectric Point: 7.4417

>NTDB_id=676272 MRO13_RS01405 WP_154169995.1 306655..307662(+) (pilM) [Vibrio metschnikovii strain 20CO01TI02]
MSKSLVIGVDIGHHSIKAVALKPNGDNYSLHGCTEIVITDDIFADNDTLNYQKIVKKLKELKKSLPLFSRKVAIALPDTA
VISKVLQIDSDLDPREKEFAIYQAFSHQSPLPMEELSLDFVPLPIQAFAKNRTATYQVYATRREVVASRLSAIQQAGLEA
TLLDVQLHGLAQIWQQVSQAQQRSNWMLVDVGYTQTTLCIDFADKAPFYKDIALGTRWFELPSLAETMARSDERNQWLFN
RLFVERLTRQLQLFTSVHGQQSLAGIWLSGGGAMMDGLAETITEQLGLPCERFAPLATFVNKITQRKRLNDQPKFTTAAG
LALRGIAWLESGHVA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=676272 MRO13_RS01405 WP_154169995.1 306655..307662(+) (pilM) [Vibrio metschnikovii strain 20CO01TI02]
ATGAGTAAATCATTAGTTATTGGTGTCGATATCGGTCACCACAGCATCAAAGCTGTGGCTCTTAAACCAAATGGCGACAA
TTATTCGCTACACGGTTGCACAGAAATCGTCATTACTGACGACATTTTCGCCGATAACGACACGTTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGAGTTTGCCGCTATTTAGTCGCAAGGTTGCGATTGCACTGCCTGATACGGCG
GTAATTAGTAAAGTATTACAAATAGATAGCGACTTAGACCCTCGTGAAAAAGAGTTTGCTATTTATCAGGCTTTTTCTCA
TCAATCGCCGTTGCCGATGGAAGAACTGAGCTTAGATTTTGTTCCCTTACCGATTCAAGCATTCGCTAAAAACCGAACTG
CGACCTATCAGGTGTATGCCACCCGAAGAGAAGTTGTCGCGAGTCGATTATCGGCGATTCAACAAGCCGGACTGGAAGCA
ACCTTACTGGATGTGCAATTACATGGTCTGGCTCAGATTTGGCAGCAGGTGAGTCAGGCTCAACAGCGCTCGAATTGGAT
GTTGGTCGATGTTGGTTATACCCAAACAACGTTATGTATCGATTTTGCCGATAAAGCGCCGTTTTATAAGGATATTGCTT
TAGGTACACGCTGGTTTGAATTGCCGTCTCTGGCGGAAACGATGGCGCGCAGCGATGAACGTAACCAATGGCTATTCAAT
CGATTATTTGTCGAACGCCTAACCCGTCAACTGCAGTTATTTACTTCCGTTCATGGTCAGCAAAGCCTAGCGGGGATCTG
GTTATCTGGTGGCGGGGCGATGATGGATGGGCTAGCAGAAACCATCACCGAGCAGCTTGGATTACCCTGTGAACGGTTCG
CGCCATTAGCGACTTTTGTCAACAAAATCACTCAACGTAAGCGGTTAAATGATCAGCCTAAATTCACGACGGCTGCTGGT
CTTGCGTTGCGAGGAATAGCATGGTTGGAGAGTGGGCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

60.177

100

0.609
  pilM Vibrio campbellii strain DS40M4

45.97

100

0.46