Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MRO13_RS01415 Genome accession   NZ_CP095311
Coordinates   308238..308828 (+) Length   196 a.a.
NCBI ID   WP_154169997.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303238..313828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS01400 (MRO13_01400) - 304033..306513 (-) 2481 WP_154169993.1 PBP1A family penicillin-binding protein -
  MRO13_RS01405 (MRO13_01405) pilM 306655..307662 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  MRO13_RS01410 (MRO13_01410) pilN 307652..308245 (+) 594 WP_110167721.1 PilN domain-containing protein Machinery gene
  MRO13_RS01415 (MRO13_01415) pilO 308238..308828 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRO13_RS01420 (MRO13_01420) pilP 308818..309339 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  MRO13_RS01425 (MRO13_01425) pilQ 309364..311085 (+) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  MRO13_RS01430 (MRO13_01430) aroK 311272..311790 (+) 519 WP_040903956.1 shikimate kinase AroK -
  MRO13_RS01435 (MRO13_01435) aroB 311819..312904 (+) 1086 WP_004394485.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22373.53 Da        Isoelectric Point: 4.5506

>NTDB_id=676274 MRO13_RS01415 WP_154169997.1 308238..308828(+) (pilO) [Vibrio metschnikovii strain 20CO01TI02]
MANFRDLDIDELLEWPLNAQLTVIVLVMVVIQVIGYGMYLSPKQEALEGLNQQEQSLKSLVRIKANKVAALPQLQTQLDE
LAERYDFLLRQLPAQQELASLLASVNELGLEHGLTFTRIDWGEKQPQAFLYRLPLNIELTGSYHNIGDFSQAIATLPRII
NVDDVDWQRVSQESSTLHFRVRAYTYQFKAEAGDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=676274 MRO13_RS01415 WP_154169997.1 308238..308828(+) (pilO) [Vibrio metschnikovii strain 20CO01TI02]
ATGGCTAATTTTCGTGATCTGGATATCGATGAATTGCTTGAATGGCCGCTCAACGCACAACTGACGGTGATTGTGTTGGT
CATGGTAGTGATTCAAGTCATCGGCTATGGCATGTATTTATCACCTAAACAAGAGGCACTCGAAGGGCTCAATCAGCAAG
AGCAGAGTCTTAAATCATTAGTACGCATTAAGGCCAATAAAGTCGCGGCATTACCTCAGTTGCAAACTCAATTGGATGAG
TTAGCTGAGCGTTATGATTTTTTATTGCGCCAACTCCCAGCACAGCAAGAGCTGGCCAGTTTGCTTGCTTCCGTCAACGA
GCTGGGATTAGAACATGGGTTAACCTTTACTCGAATTGATTGGGGAGAAAAGCAACCGCAGGCATTTTTGTATCGCTTAC
CATTGAATATTGAACTGACGGGAAGTTACCACAACATTGGCGATTTTTCTCAGGCGATAGCCACGCTACCAAGAATCATC
AATGTTGATGATGTGGATTGGCAAAGAGTCAGTCAAGAGAGCAGTACCTTACATTTCAGAGTACGGGCTTACACCTACCA
ATTTAAAGCGGAGGCAGGCGATGAGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

72.449

100

0.724
  pilO Vibrio campbellii strain DS40M4

67.725

96.429

0.653