Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ISX51_RS04070 Genome accession   NZ_CP094659
Coordinates   808134..808544 (-) Length   136 a.a.
NCBI ID   WP_281053617.1    Uniprot ID   -
Organism   Vibrio lentus strain ED252     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803134..813544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS04050 (ISX51_04050) coaE 803646..804260 (-) 615 WP_102306386.1 dephospho-CoA kinase -
  ISX51_RS04055 (ISX51_04055) pilD 804263..805132 (-) 870 WP_017106882.1 A24 family peptidase Machinery gene
  ISX51_RS04060 (ISX51_04060) pilC 805200..806429 (-) 1230 WP_281053369.1 type II secretion system F family protein Machinery gene
  ISX51_RS04065 (ISX51_04065) pilB 806444..808132 (-) 1689 WP_281053370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISX51_RS04070 (ISX51_04070) pilA 808134..808544 (-) 411 WP_281053617.1 pilin Machinery gene
  ISX51_RS04075 (ISX51_04075) nadC 808799..809689 (-) 891 WP_102382993.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ISX51_RS04080 (ISX51_04080) ampD 809773..810372 (+) 600 WP_281053371.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ISX51_RS04085 (ISX51_04085) pdhR 810856..811617 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13826.66 Da        Isoelectric Point: 4.6320

>NTDB_id=670482 ISX51_RS04070 WP_281053617.1 808134..808544(-) (pilA) [Vibrio lentus strain ED252]
MNNKNKRTNQKGFTLIELMIVVAIIGALSAIAVPAYKDYVSKSQASSALATLKALITPAELLIQEEGSISGGVSALGVSA
SSNTLGTISASGTTISFSFVDGSLDGDSMTMTRNADTGWSCSLSASSAIPSIEGCN

Nucleotide


Download         Length: 411 bp        

>NTDB_id=670482 ISX51_RS04070 WP_281053617.1 808134..808544(-) (pilA) [Vibrio lentus strain ED252]
ATGAATAACAAGAACAAAAGAACGAACCAGAAAGGCTTTACGCTAATTGAATTGATGATTGTAGTTGCGATTATTGGTGC
TTTATCAGCGATTGCTGTTCCTGCTTACAAAGACTATGTATCAAAAAGCCAAGCATCTTCAGCATTAGCAACACTAAAAG
CACTAATTACACCAGCTGAATTATTGATCCAAGAAGAAGGCAGCATTAGTGGCGGCGTTAGTGCATTGGGTGTTTCAGCT
AGCTCAAATACTTTAGGGACTATCTCAGCTTCAGGAACTACCATTTCATTTTCATTTGTAGACGGTAGCCTTGATGGTGA
CAGTATGACCATGACAAGGAATGCGGATACTGGTTGGTCATGTTCACTATCAGCCTCAAGCGCAATCCCTTCAATAGAAG
GCTGCAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-123)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50

100

0.515
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.136

100

0.434
  pilA Vibrio cholerae strain A1552

40.136

100

0.434
  pilA Vibrio cholerae C6706

40.136

100

0.434
  pilA Pseudomonas aeruginosa PAK

43.609

97.794

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.353

97.794

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.458

96.324

0.39
  pilA/pilA1 Eikenella corrodens VA1

36.957

100

0.375