Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ISX51_RS04065 Genome accession   NZ_CP094659
Coordinates   806444..808132 (-) Length   562 a.a.
NCBI ID   WP_281053370.1    Uniprot ID   -
Organism   Vibrio lentus strain ED252     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801444..813132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS04035 (ISX51_04035) rplS 801794..802147 (+) 354 WP_009847607.1 50S ribosomal protein L19 -
  ISX51_RS04040 (ISX51_04040) yacG 802600..802797 (-) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  ISX51_RS04045 (ISX51_04045) zapD 802879..803619 (-) 741 WP_017108712.1 cell division protein ZapD -
  ISX51_RS04050 (ISX51_04050) coaE 803646..804260 (-) 615 WP_102306386.1 dephospho-CoA kinase -
  ISX51_RS04055 (ISX51_04055) pilD 804263..805132 (-) 870 WP_017106882.1 A24 family peptidase Machinery gene
  ISX51_RS04060 (ISX51_04060) pilC 805200..806429 (-) 1230 WP_281053369.1 type II secretion system F family protein Machinery gene
  ISX51_RS04065 (ISX51_04065) pilB 806444..808132 (-) 1689 WP_281053370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISX51_RS04070 (ISX51_04070) pilA 808134..808544 (-) 411 WP_281053617.1 pilin Machinery gene
  ISX51_RS04075 (ISX51_04075) nadC 808799..809689 (-) 891 WP_102382993.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ISX51_RS04080 (ISX51_04080) ampD 809773..810372 (+) 600 WP_281053371.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ISX51_RS04085 (ISX51_04085) pdhR 810856..811617 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62140.83 Da        Isoelectric Point: 5.0429

>NTDB_id=670481 ISX51_RS04065 WP_281053370.1 806444..808132(-) (pilB) [Vibrio lentus strain ED252]
MGLTNLPTILRQANLLSLTQEQAVAEHVQASGVSTPEALLVLDIFTGESLAHNIQTIFGLPLVQLGTTDYEALCEQLGLR
ELITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELA
NLVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEERFRVRLRCDGILVEIQQPASHLSRRLSARLK
ILAKLDIAERRLPQDGRIKLRLNDQLSIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGM
ILMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGVNQVQVAPKIGFGFAEALRSFLRQDPDVVMVGEIRDLE
TAEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADDSPDIFLRHSIPNSQ
TIYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKSSLIDKPNALTIENLARREGMRTLQESGLDKLLEGTTSYQELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=670481 ISX51_RS04065 WP_281053370.1 806444..808132(-) (pilB) [Vibrio lentus strain ED252]
TTGGGACTAACCAACCTCCCAACGATTCTTCGTCAGGCTAACTTACTTAGCCTGACTCAGGAACAAGCAGTTGCGGAACA
TGTACAAGCTTCAGGTGTTTCGACACCTGAAGCTTTACTCGTTCTAGACATCTTTACTGGCGAGAGTCTCGCACACAACA
TTCAGACCATCTTCGGCTTGCCGTTGGTACAACTTGGCACTACGGACTATGAAGCTTTATGCGAACAGTTAGGACTTCGT
GAGCTGATCACCAAGTATCGCGCAATTCCAATTTCAGTTTCTAGCTCCACTCTCACACTCGCTTCTGCCGACCCTACGGA
TTTACAAGCTGAAGATGACTTTCGATTCGCGACCGGATTGCAGATTGAGTTAGTTGTCGCTAACTACTCAGAACTAGAAG
GTGCAATACGAAAGCTGTATGGACGTTCAATTTCAGGGCAAGACTCCAAGCGCAAAGAGATCACCCAAGATGAACTCGCC
AACCTGGTTGAAGTCTCTGACGATGAGATGACTTCAATTGAAGACCTCAGCCAAGACGACTCCCCTGTTAGCCGTTTTAT
CAATCAAATATTAGTTGATGCGGTACGTAAAGGTGCATCAGATATCCACTTTGAGCCTTATGAAGAACGCTTCCGAGTAC
GTCTGCGTTGTGATGGCATCCTGGTTGAAATACAACAACCCGCATCCCATTTAAGCCGCCGTTTATCTGCTCGCTTAAAG
ATCCTCGCCAAGCTGGATATCGCTGAGCGTCGCCTGCCGCAAGATGGTCGTATTAAGTTGCGGTTAAACGATCAACTCTC
GATCGATATGCGAGTATCGACGCTACCGACATTGTGGGGAGAAAAGATCGTACTGCGCCTTCTAGACAGCAGCGCTGCCA
ATTTAGATATCGATAAGTTAGGTTACAGCGAAGATCAAAAAGCACTCTATCTCAATGCATTGAAACGCCCGCAAGGGATG
ATCTTAATGACAGGCCCAACAGGCAGTGGTAAAACCGTTTCTCTCTACACAGGCCTTCGAGTGCTGAACACCACTGAGCG
CAACATCTCAACAGCCGAAGATCCGGTCGAGATCAATCTATGTGGTGTCAATCAAGTTCAAGTCGCACCCAAAATCGGTT
TTGGGTTTGCTGAAGCATTGCGATCATTTTTACGACAAGACCCCGATGTGGTAATGGTCGGGGAGATCCGCGATTTAGAA
ACCGCAGAGATCGCGATCAAAGCCTCGCAAACCGGTCACTTAGTCCTTTCGACATTGCACACCAACTCCGCCGCAGAAAC
CGTGACTCGATTGGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCATCATTAAGCTTGATTATAGCCCAACGACTAG
CAAGACGATTGTGCAATCACTGTAAAGCCGCTGACGACTCTCCGGACATATTTCTGCGTCACTCTATCCCTAACAGCCAA
ACGATCTACAAAGCCAATCCTCAGGGGTGTAATGAGTGTAATCAGGGATACTCTGGGCGAGTGGGTATCTATGAAGTTAT
GCCGTTTAGCGACCAACTGAAAAGCAGCCTGATCGACAAACCGAATGCGTTAACTATTGAAAACTTGGCGCGCCGAGAAG
GCATGCGAACACTGCAAGAATCAGGACTGGATAAATTACTGGAAGGCACCACCAGTTATCAAGAACTACAACGTGTTCTG
TACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-142)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.535

100

0.737
  pilB Vibrio parahaemolyticus RIMD 2210633

70

99.644

0.698
  pilB Vibrio campbellii strain DS40M4

69.519

99.822

0.694
  pilB Acinetobacter baumannii D1279779

50.482

92.349

0.466
  pilB Acinetobacter baylyi ADP1

49.326

92.349

0.456
  pilB Legionella pneumophila strain ERS1305867

48.855

93.238

0.456
  pilF Neisseria gonorrhoeae MS11

43.423

98.754

0.429