Detailed information NTG0066842

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PBDP_RS10785
• Genome accession: NZ_AP014703
• Coordinates: 2446097..2447212 (+)
• Length: 371 a.a.
• NCBI ID: WP_109751850.1
• Uniprot ID: -
• Organism: Pseudomonas sp. St290
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2441097..2452212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PBDP_RS10770 (PBDP_2131)	-	2442044..2442964 (+)	921	WP_212802218.1	sugar kinase	-
PBDP_RS10775 (PBDP_2132)	-	2443103..2443378 (-)	276	WP_019690952.1	peptidylprolyl isomerase	-
PBDP_RS10780 (PBDP_2133)	-	2443427..2445964 (-)	2538	WP_212802219.1	PAS domain-containing protein	-
PBDP_RS10785 (PBDP_2134)	pilU	2446097..2447212 (+)	1116	WP_109751850.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PBDP_RS10790 (PBDP_2135)	-	2447377..2449332 (-)	1956	WP_212802220.1	acetoacetate--CoA ligase	-
PBDP_RS10795 (PBDP_2136)	hbdH	2449490..2450266 (-)	777	WP_116832006.1	3-hydroxybutyrate dehydrogenase	-
PBDP_RS10800 (PBDP_2137)	-	2450274..2451665 (-)	1392	WP_212802221.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41201.16 Da
• Isoelectric Point: 6.2506

>NTDB_id=66842 PBDP_RS10785 WP_109751850.1 2446097..2447212(+) (pilU) [Pseudomonas sp. St290]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLGNEACKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVAEGAIDEEEALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=66842 PBDP_RS10785 WP_109751850.1 2446097..2447212(+) (pilU) [Pseudomonas sp. St290]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGGGCGCCACCGTG
CGCGCGTTTCGAGGGCGTGCTCAAGCCCTTGGGTAATGAAGCATGCAAGGTCGGCGACATTGCCAGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGCTGGAAATGAACCTGGCGATCTCCCTGGCCGGCGTCGGGCGT
TTCCGGGTCAATATCTTCAAACAACGCAATGACGTGTCCATGGTGATACGCAACGTCAAGCTGGACATCCCGCGTTTCGA
AGACCTGAAACTGCCACGGGTGCTGCTCGATACCATCATGCAGAAGCAAGGGCTGATGCTGTTCGTCGGAGCGACGGGGT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAACCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAGCGGGAGGTCGGTGTCGACACCCGCAGTTTTCATGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTACTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACGTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTA
ATCAACTTCTTCCCGGAAGAGCGTCGGCCGCAGTTGCTCAACGACTTGGGCAACAACCTCAAGGCCTTCGTTTCCCAACG
CCTGGTGCGCACCCGTACCGGCCAGCGTCGGGCGGCGGTGGAGGTAATGCTAGGCTCGCCCACGGTGGCCGACCTGATCC
GCCGCAATGAGCTGGGCGAGCTCAAGGGCATCATGGAAAAGTCCGAAGAGTTGGGCATGCAGACCTTTGACCAGGCCTTG
TTCAACCTGGTGGCAGAGGGAGCCATCGATGAGGAAGAAGCGCTGAAAAACGCTGATTCGGCGAACAACCTGCGCTTGCG
GCTGAAGTTGCATGCCGAGTCGGGCGCGGCGCCACCCGCCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	51.791	97.844	0.507
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.692	98.922	0.383
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Acinetobacter baumannii D1279779	40.419	90.027	0.364
pilT	Acinetobacter baumannii strain A118	40.419	90.027	0.364
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364
pilT	Acinetobacter nosocomialis M2	40.12	90.027	0.361