Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   MNO09_RS20025 Genome accession   NZ_CP093325
Coordinates   3411087..3411425 (-) Length   112 a.a.
NCBI ID   WP_088107121.1    Uniprot ID   A0A2B6RSE9
Organism   Bacillus sp. N5-665     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3411087..3442818 3411087..3411425 within 0


Gene organization within MGE regions


Location: 3411087..3442818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO09_RS20025 (MNO09_19905) ssb 3411087..3411425 (-) 339 WP_088107121.1 single-stranded DNA-binding protein Machinery gene
  MNO09_RS20030 (MNO09_19910) - 3411584..3411838 (-) 255 WP_000975142.1 DUF4318 domain-containing protein -
  MNO09_RS20035 (MNO09_19915) - 3411993..3412724 (-) 732 WP_001260655.1 Bax inhibitor-1/YccA family protein -
  MNO09_RS20040 (MNO09_19920) - 3412800..3414695 (-) 1896 WP_000783182.1 ABC-F family ATP-binding cassette domain-containing protein -
  MNO09_RS20045 (MNO09_19925) - 3414692..3415339 (-) 648 WP_000165957.1 HD domain-containing protein -
  MNO09_RS20050 (MNO09_19930) - 3415449..3416270 (+) 822 WP_088107120.1 hypothetical protein -
  MNO09_RS20055 (MNO09_19935) - 3416573..3417845 (-) 1273 Protein_3521 XkdF-like putative serine protease domain-containing protein -
  MNO09_RS20060 (MNO09_19940) - 3417959..3418477 (-) 519 WP_241950201.1 phage minor head protein -
  MNO09_RS20065 (MNO09_19945) - 3418591..3420070 (-) 1480 Protein_3523 phage portal protein -
  MNO09_RS20070 (MNO09_19950) - 3420286..3421137 (+) 852 WP_241950045.1 exosporium leader peptide-containing protein -
  MNO09_RS20075 (MNO09_19955) - 3421344..3422625 (-) 1282 Protein_3525 PBSX family phage terminase large subunit -
  MNO09_RS20080 (MNO09_19960) terS 3422618..3423442 (-) 825 WP_088107118.1 phage terminase small subunit -
  MNO09_RS20085 (MNO09_19965) - 3423464..3423643 (-) 180 WP_000390758.1 hypothetical protein -
  MNO09_RS20090 (MNO09_19970) - 3423762..3423965 (+) 204 WP_080014171.1 hypothetical protein -
  MNO09_RS20095 (MNO09_19975) - 3423978..3424202 (+) 225 WP_000265596.1 hypothetical protein -
  MNO09_RS20100 (MNO09_19980) - 3424298..3424840 (-) 543 WP_000673904.1 site-specific integrase -
  MNO09_RS20105 (MNO09_19985) - 3425029..3425172 (+) 144 WP_229181210.1 DUF3956 family protein -
  MNO09_RS20110 (MNO09_19990) - 3426114..3426809 (+) 696 WP_001294973.1 CsxC family protein -
  MNO09_RS20115 (MNO09_19995) - 3427012..3427266 (-) 255 WP_033657800.1 hypothetical protein -
  MNO09_RS20120 (MNO09_20000) - 3427720..3428346 (+) 627 WP_000069270.1 hypothetical protein -
  MNO09_RS20125 (MNO09_20005) - 3428600..3429142 (-) 543 WP_001012158.1 site-specific integrase -
  MNO09_RS20130 (MNO09_20010) - 3429142..3429624 (-) 483 WP_000166159.1 ArpU family phage packaging/lysis transcriptional regulator -
  MNO09_RS20135 (MNO09_20015) - 3429649..3429819 (-) 171 WP_000866149.1 hypothetical protein -
  MNO09_RS20140 (MNO09_20020) - 3429979..3430635 (-) 657 WP_000431217.1 hypothetical protein -
  MNO09_RS20145 (MNO09_20025) - 3432223..3433539 (+) 1317 WP_088107116.1 collagen-like protein -
  MNO09_RS20150 (MNO09_20030) - 3434047..3434712 (+) 666 WP_086398573.1 exosporium leader peptide-containing protein -
  MNO09_RS20155 (MNO09_20035) - 3435007..3435873 (+) 867 WP_000796374.1 hypothetical protein -
  MNO09_RS20160 (MNO09_20040) - 3436427..3437920 (+) 1494 WP_241950046.1 exosporium leader peptide-containing protein -
  MNO09_RS20165 (MNO09_20045) - 3438093..3438284 (-) 192 WP_000511362.1 DUF3954 domain-containing protein -
  MNO09_RS20170 (MNO09_20050) - 3438356..3438718 (-) 363 WP_001125993.1 hypothetical protein -
  MNO09_RS20175 (MNO09_20055) - 3438729..3439736 (-) 1008 WP_061673109.1 DnaD domain protein -
  MNO09_RS20180 (MNO09_20060) - 3439854..3440078 (-) 225 WP_000161462.1 hypothetical protein -
  MNO09_RS20185 (MNO09_20065) - 3440064..3440360 (+) 297 Protein_3547 ImmA/IrrE family metallo-endopeptidase -
  MNO09_RS20190 (MNO09_20070) - 3440381..3441517 (+) 1137 WP_000678744.1 site-specific integrase -
  MNO09_RS20195 (MNO09_20075) - 3442318..3442818 (+) 501 WP_000069070.1 hypothetical protein -

Sequence

Protein


Download         Length: 112 a.a.        Molecular weight: 12922.86 Da        Isoelectric Point: 9.4109

>NTDB_id=663613 MNO09_RS20025 WP_088107121.1 3411087..3411425(-) (ssb) [Bacillus sp. N5-665]
MMNRVVLIGRLTKEPELYYTKQSVAYARICVAVNKGFRNSLGEQQVDFINCVVWRKSAENVTEYCKKGSLVGITGRIHTR
NYEDDQGKRIYITEVLIESITFLEKRREGASQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=663613 MNO09_RS20025 WP_088107121.1 3411087..3411425(-) (ssb) [Bacillus sp. N5-665]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAGCAAAGTGTCGCTTATGC
ACGAATATGTGTTGCGGTGAATAAAGGTTTTCGAAATAGTTTAGGTGAGCAACAAGTAGATTTTATTAATTGTGTCGTTT
GGAGAAAATCGGCTGAGAATGTAACTGAATATTGTAAGAAGGGGTCACTTGTTGGAATTACCGGACGTATTCATACGAGG
AATTACGAGGATGATCAAGGAAAGAGAATATATATAACGGAAGTTCTGATTGAGAGCATTACATTTTTGGAGAAAAGGCG
GGAGGGGGCATCGCAATAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B6RSE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

51.261

100

0.545
  ssbA Bacillus subtilis subsp. subtilis str. 168

57.547

94.643

0.545
  ssbB Bacillus subtilis subsp. subtilis str. 168

50.442

100

0.509
  ssbB Streptococcus sobrinus strain NIDR 6715-7

46.364

98.214

0.455
  ssbB Lactococcus lactis subsp. cremoris KW2

47.619

93.75

0.446
  ssbA Streptococcus mutans UA159

42.727

98.214

0.42
  ssbB/cilA Streptococcus pneumoniae TIGR4

41.818

98.214

0.411
  ssbB/cilA Streptococcus mitis NCTC 12261

41.818

98.214

0.411
  ssbB/cilA Streptococcus pneumoniae D39

40.909

98.214

0.402
  ssbB/cilA Streptococcus pneumoniae R6

40.909

98.214

0.402
  ssbB/cilA Streptococcus pneumoniae Rx1

40.909

98.214

0.402
  ssbB/cilA Streptococcus mitis SK321

40.909

98.214

0.402