Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MME54_RS23330 Genome accession   NZ_CP092918
Coordinates   5190267..5191484 (+) Length   405 a.a.
NCBI ID   WP_054988237.1    Uniprot ID   A0A0P9R5J1
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5185267..5196484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS23315 (MME54_23315) - 5186274..5187461 (-) 1188 WP_420917730.1 O-antigen ligase family protein -
  MME54_RS23320 (MME54_23320) pilA 5187948..5188343 (-) 396 WP_082441279.1 pilin Machinery gene
  MME54_RS23325 (MME54_23325) pilB 5188570..5190264 (+) 1695 WP_054988238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MME54_RS23330 (MME54_23330) pilC 5190267..5191484 (+) 1218 WP_054988237.1 type II secretion system F family protein Machinery gene
  MME54_RS23335 (MME54_23335) pilD 5191486..5192358 (+) 873 WP_054988236.1 prepilin peptidase Machinery gene
  MME54_RS23340 (MME54_23340) coaE 5192355..5192978 (+) 624 WP_054988235.1 dephospho-CoA kinase -
  MME54_RS23345 (MME54_23345) yacG 5192975..5193184 (+) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  MME54_RS23350 (MME54_23350) - 5193651..5194813 (+) 1163 WP_103715205.1 IS3 family transposase -
  MME54_RS23355 (MME54_23355) - 5194822..5195013 (-) 192 Protein_4636 hypothetical protein -
  MME54_RS23360 (MME54_23360) - 5195210..5195839 (+) 630 WP_054988352.1 DUF1780 domain-containing protein -
  MME54_RS23365 (MME54_23365) - 5195836..5196369 (+) 534 WP_054988351.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43950.76 Da        Isoelectric Point: 10.1097

>NTDB_id=661135 MME54_RS23330 WP_054988237.1 5190267..5191484(+) (pilC) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPIKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQTVFAGFGAQLPAFTLMVIGLSGFVEQWWLAIIGLFFAGIFLFK
RAYKQSQNFRDSVDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFT
LGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=661135 MME54_RS23330 WP_054988237.1 5190267..5191484(+) (pilC) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGGCCAGTAAGGCAGTTAAAGTCATCGTTTACACTTGGGAAGGTGTCGACAAAAAGGGCACTAAAACCAGCGGCGAATT
GAGCGGCCATAACTTGGCACTCGTAAAAGCCCAGTTGCGCAAGCAAGGCATCAATCCGATTAAGGTGCGCAAGAAATCCG
TATCGATCTTCGGCAAGGGAAAAAAAATCAAGCCTCTGGATATAGCTTTTTTTTCTAGGCAAATGGCAACGATGATGAAA
GCAGGTGTGCCCCTGCTTCAGTCATTCGATATCATCAGCGAAGGTGCCGAGAACCCTAACATGCGCGCATTGGTGAACTC
GCTCAAACAGGAGGTTTCTGCAGGCAACAGCTTTGCAACAGCCTTACGACAAAAACCTGAGTACTTCGACGACCTATTTT
GCAATCTAGTAGATGCCGGGGAACAAGCCGGGGCTCTTGAAAGCTTGCTGGACCGTGTCGCAAGTTATAAGGAAAAGACA
GAAAGACTTAAAGCAAAAATCAAAAAAGCAATGACCTATCCTGCAGCCGTGTTGATAGTAGCGATTATAGTTTCAGGAAT
TTTGCTTATAAAGGTAGTGCCACAATTTCAGACTGTGTTCGCTGGCTTTGGCGCGCAGCTGCCTGCATTTACACTAATGG
TCATCGGGCTTTCAGGCTTTGTGGAGCAATGGTGGCTGGCCATTATCGGTTTATTCTTCGCAGGTATTTTTTTGTTCAAA
CGAGCCTACAAGCAGTCACAAAACTTCCGTGACAGCGTTGATCGTTTTTTGTTGAAAGTTCCGATCATTGGACCCTTGAT
TTTCAAGTCATCTGTAGCTCGGTACGCCCGCACTCTTGCAACGACTTTTGCTGCAGGCGTGCCATTGGTCGAAGCTTTGG
ACTCCGTAGCAGGCGCCACTGGGAACGTGGTTTTCAAAAATGCTGTTAACAAGGTCAAGCAAGATGTGTCCACGGGTATG
CAATTGAATTTTTCTATGCGCTCGACGGGTGTCTTCCCCAGCTTGGCGATCCAGATGACTGCGATCGGCGAAGAGTCTGG
TGCGCTGGACACCATGCTGGATAAAGTCGCAACCTATTATGAAGATGAGGTTGATAACATGGTCGACAGTTTGACTAGTC
TGATGGAACCAATGATCATGGCGGTGCTGGGAGTTATAGTGGGCGGTCTTGTAATCGCCATGTATCTCCCCATTTTCACA
CTTGGAAATGCAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9R5J1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

50.985

100

0.511
  pilG Neisseria gonorrhoeae MS11

43.325

98.025

0.425
  pilG Neisseria meningitidis 44/76-A

43.325

98.025

0.425
  pilC Vibrio campbellii strain DS40M4

43.003

97.037

0.417
  pilC Vibrio cholerae strain A1552

41.646

99.012

0.412
  pilC Thermus thermophilus HB27

38.903

99.012

0.385