Detailed information NTG0661136

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MME54_RS23335
• Genome accession: NZ_CP092918
• Coordinates: 5191486..5192358 (+)
• Length: 290 a.a.
• NCBI ID: WP_054988236.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. helianthi strain LMG 5067
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5186486..5197358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MME54_RS23320 (MME54_23320)	pilA	5187948..5188343 (-)	396	WP_082441279.1	pilin	Machinery gene
MME54_RS23325 (MME54_23325)	pilB	5188570..5190264 (+)	1695	WP_054988238.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MME54_RS23330 (MME54_23330)	pilC	5190267..5191484 (+)	1218	WP_054988237.1	type II secretion system F family protein	Machinery gene
MME54_RS23335 (MME54_23335)	pilD	5191486..5192358 (+)	873	WP_054988236.1	prepilin peptidase	Machinery gene
MME54_RS23340 (MME54_23340)	coaE	5192355..5192978 (+)	624	WP_054988235.1	dephospho-CoA kinase	-
MME54_RS23345 (MME54_23345)	yacG	5192975..5193184 (+)	210	WP_007251656.1	DNA gyrase inhibitor YacG	-
MME54_RS23350 (MME54_23350)	-	5193651..5194813 (+)	1163	WP_103715205.1	IS3 family transposase	-
MME54_RS23355 (MME54_23355)	-	5194822..5195013 (-)	192	Protein_4636	hypothetical protein	-
MME54_RS23360 (MME54_23360)	-	5195210..5195839 (+)	630	WP_054988352.1	DUF1780 domain-containing protein	-
MME54_RS23365 (MME54_23365)	-	5195836..5196369 (+)	534	WP_054988351.1	MOSC domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31981.19 Da
• Isoelectric Point: 8.1527

>NTDB_id=661136 MME54_RS23335 WP_054988236.1 5191486..5192358(+) (pilD) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPVFNLILPHSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGYGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=661136 MME54_RS23335 WP_054988236.1 5191486..5192358(+) (pilD) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCTCCCCTGGCCTTCGTCACCACCTGCTGCCTACTCGGCCTAATCGTCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGCGCAATCACGGGAAATGCTCGGCC
TCCCTGCCGAACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCAAGCTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAAAATCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTATCAGCCTACGTCGCCTGGCATTTCGGCTACGGCTGGCAGGCGGCGG
CGATGCTGGTGTTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGACGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCATTGCTGTGGCTGGGCCTGATAGTCAACGCTTTCGGGCTGTTCACCTCGCTGAATGATGCGCTGTGGGGCGCAGT
GGCAGGCTATCTGGCGCTCTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGCTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCGTCGCTGGTGGGC
GCGGTGCTAGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.064	97.586	0.528
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.19	94.483	0.493
pilD	Neisseria gonorrhoeae MS11	51.866	92.414	0.479