Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MME54_RS23325 Genome accession   NZ_CP092918
Coordinates   5188570..5190264 (+) Length   564 a.a.
NCBI ID   WP_054988238.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5183570..5195264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS23300 (MME54_23300) - 5183613..5184305 (-) 693 WP_054986683.1 DUF433 domain-containing protein -
  MME54_RS23305 (MME54_23305) - 5184469..5184792 (+) 324 Protein_4626 DUF1523 family protein -
  MME54_RS23310 (MME54_23310) - 5184841..5186118 (-) 1278 WP_054988239.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  MME54_RS23315 (MME54_23315) - 5186274..5187461 (-) 1188 WP_420917730.1 O-antigen ligase family protein -
  MME54_RS23320 (MME54_23320) pilA 5187948..5188343 (-) 396 WP_082441279.1 pilin Machinery gene
  MME54_RS23325 (MME54_23325) pilB 5188570..5190264 (+) 1695 WP_054988238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MME54_RS23330 (MME54_23330) pilC 5190267..5191484 (+) 1218 WP_054988237.1 type II secretion system F family protein Machinery gene
  MME54_RS23335 (MME54_23335) pilD 5191486..5192358 (+) 873 WP_054988236.1 prepilin peptidase Machinery gene
  MME54_RS23340 (MME54_23340) coaE 5192355..5192978 (+) 624 WP_054988235.1 dephospho-CoA kinase -
  MME54_RS23345 (MME54_23345) yacG 5192975..5193184 (+) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  MME54_RS23350 (MME54_23350) - 5193651..5194813 (+) 1163 WP_103715205.1 IS3 family transposase -
  MME54_RS23355 (MME54_23355) - 5194822..5195013 (-) 192 Protein_4636 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62450.66 Da        Isoelectric Point: 6.2549

>NTDB_id=661134 MME54_RS23325 WP_054988238.1 5188570..5190264(+) (pilB) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MTDAVLTGLAKQLVLAELLTESVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCNHCKKELDIPRETLIQEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=661134 MME54_RS23325 WP_054988238.1 5188570..5190264(+) (pilB) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTCCTTACTGAATCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAGTTAGTCAAAAGCCTGACGTTGG
CAGAGATGGCTTCCGATCAGTTTGGCGTGCCGTTTATGGACCTCGCCAGCCTGGACAAGGAAAGCCAGCCAAAGGGGCTG
GTAAGTGAAAAGCTGGTTCGGCAACACCACGCGCTACCGCTATGGCGACGCGGTAACAAGCTTTTTATCGGAATTTCTGA
TCCGACCAACCATCAGGCTGTGACAGACATTCAGTTCAGTACTGGCCTGAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACCACTGCGATAGATCGATTTTTTGACAGTGATAGCGGTCTTGGCAACCTGGAAGATGTCGATCTGGGCTTGGAT
ATCGAACCGGCTGATGGCAAGGAGACGTCACTCGCGACGCAGAATGACGCTGACGATGCCCCTGTGGTCCGTTTCGTCAA
CAAAATGCTGCTGGATGCCATCAGGTTAGGATCGTCCGACCTGCACTTCGAACCCTACGAAAAGATCTTTCGCGTGCGCT
TACGCACTGACGGCATTTTGCATGAAGTCGCCAGGCCGCCGATTCATCTGGCGAACCGAATTGCTGCACGCTTGAAAGTC
ATGGCCAGCCTCGATATCTCGGAGCGACGCAAACCGCAGGACGGTCGGGTCAAGTTGCGAGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAACACCTTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACAAGTGCGCAGA
TGGGTATTGATGCTCTGGGCTACGAGCCGGAGCAAAAAGCACTGTATCTGGAGGCCCTGAAACAACCGCAGGGCATGATT
CTTGTGACTGGCCCTACCGGTTCCGGCAAGACCGTTTCGCTGTATACGGGCCTTAATATTCTCAATACCGTGGATATCAA
CATTTCCACAGCCGAAGACCCGGTAGAGATCAACCTGGAAGGTATCAATCAGGTCAACGTCAACCCACGCCAAGGATTGG
ACTTTTCGCAGGCATTGCGCGCATTCCTGCGTCAAGATCCTGACGTGATCATGGTCGGCGAGATTCGCGACCTGGAAACA
GCCGAAATCGCCATCAAGGCGTCCCAGACCGGCCATATGGTGCTGTCCACCCTGCACACCAATAGCGCGGCAGAAACCTT
GACGCGACTGCATCACATGGGTGTAGCCGCATTCAATATTGCCACGGCAATCAACTTGATTATTGCGCAACGACTTGCGC
GCAAGCTGTGCAACCATTGCAAGAAGGAACTCGATATTCCTCGCGAGACACTGATTCAGGAAGGTTTTCCAGAAGCGAAG
ATTGGCACTTTCAAGATCTATGGTCCGGTGGGCTGCGAACATTGCAATGGTGGCTACAGGGGAAGGGTCGGTATTTATGA
AGTCGTCAAGAAAACAGCAGAGCTAGAGCGCATCATCATGGAAGAGGGTAACTCACTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAACGACCTGCGAACCTCAGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTTAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.496

100

0.578
  pilB Acinetobacter baylyi ADP1

56.36

100

0.566
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

51.068

99.645

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.984

99.113

0.505
  pilB Vibrio campbellii strain DS40M4

49.647

100

0.498
  pilF Neisseria gonorrhoeae MS11

47.865

99.645

0.477
  pilF Thermus thermophilus HB27

38.869

100

0.39