Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MME54_RS23320 Genome accession   NZ_CP092918
Coordinates   5187948..5188343 (-) Length   131 a.a.
NCBI ID   WP_082441279.1    Uniprot ID   A0A0P9SDF8
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5182948..5193343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS23295 (MME54_23295) - 5183210..5183616 (-) 407 Protein_4624 hypothetical protein -
  MME54_RS23300 (MME54_23300) - 5183613..5184305 (-) 693 WP_054986683.1 DUF433 domain-containing protein -
  MME54_RS23305 (MME54_23305) - 5184469..5184792 (+) 324 Protein_4626 DUF1523 family protein -
  MME54_RS23310 (MME54_23310) - 5184841..5186118 (-) 1278 WP_054988239.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  MME54_RS23315 (MME54_23315) - 5186274..5187461 (-) 1188 WP_420917730.1 O-antigen ligase family protein -
  MME54_RS23320 (MME54_23320) pilA 5187948..5188343 (-) 396 WP_082441279.1 pilin Machinery gene
  MME54_RS23325 (MME54_23325) pilB 5188570..5190264 (+) 1695 WP_054988238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MME54_RS23330 (MME54_23330) pilC 5190267..5191484 (+) 1218 WP_054988237.1 type II secretion system F family protein Machinery gene
  MME54_RS23335 (MME54_23335) pilD 5191486..5192358 (+) 873 WP_054988236.1 prepilin peptidase Machinery gene
  MME54_RS23340 (MME54_23340) coaE 5192355..5192978 (+) 624 WP_054988235.1 dephospho-CoA kinase -
  MME54_RS23345 (MME54_23345) yacG 5192975..5193184 (+) 210 WP_007251656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 12886.68 Da        Isoelectric Point: 7.5920

>NTDB_id=661133 MME54_RS23320 WP_082441279.1 5187948..5188343(-) (pilA) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MNAQKGFTLIELMIVVAIVGILAAVAIPQYQNYVARANGASAVAMLDAAKTQVGINAQEGLSTALCTNVTMPTNGTCNAT
TGTLVSPSVGNGTSATTATLAPTLGAAGAITWTCSVSNAKSASSTCTSTGT

Nucleotide


Download         Length: 396 bp        

>NTDB_id=661133 MME54_RS23320 WP_082441279.1 5187948..5188343(-) (pilA) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGAACGCACAAAAAGGCTTTACGCTGATCGAGCTGATGATCGTTGTTGCGATTGTTGGTATTTTGGCTGCTGTGGCAAT
TCCGCAATACCAGAACTATGTCGCGCGTGCGAACGGCGCCAGCGCGGTCGCCATGCTTGATGCTGCGAAGACTCAGGTGG
GGATCAATGCTCAAGAAGGGCTTTCCACTGCTCTCTGCACTAACGTGACCATGCCTACAAATGGTACATGTAATGCTACA
ACGGGCACCTTGGTTAGCCCGTCGGTTGGGAACGGAACTTCTGCAACTACCGCTACTTTGGCTCCTACCTTGGGTGCCGC
AGGTGCCATAACTTGGACTTGTTCGGTAAGTAATGCTAAGTCTGCTTCCAGCACATGCACCTCCACTGGTACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9SDF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

43.151

100

0.481
  comP Acinetobacter baylyi ADP1

40.411

100

0.45
  pilA Pseudomonas aeruginosa PAK

50

88.55

0.443
  pilA2 Legionella pneumophila str. Paris

42.52

96.947

0.412
  pilA2 Legionella pneumophila strain ERS1305867

40.157

96.947

0.389
  pilA/pilA1 Eikenella corrodens VA1

38.168

100

0.382