Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MKK04_RS24735 Genome accession   NZ_CP092827
Coordinates   5378894..5380162 (-) Length   422 a.a.
NCBI ID   WP_233688152.1    Uniprot ID   -
Organism   Pseudomonas sp. LS.1a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5373894..5385162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKK04_RS24720 (MKK04_24720) - 5375527..5377161 (-) 1635 WP_207828293.1 AAA family ATPase -
  MKK04_RS24725 (MKK04_24725) aroB 5377177..5378274 (-) 1098 WP_015272135.1 3-dehydroquinate synthase -
  MKK04_RS24730 (MKK04_24730) aroK 5378371..5378889 (-) 519 WP_028698445.1 shikimate kinase AroK -
  MKK04_RS24735 (MKK04_24735) pilQ 5378894..5380162 (-) 1269 WP_233688152.1 secretin N-terminal domain-containing protein Machinery gene
  MKK04_RS24740 (MKK04_24740) - 5380086..5381102 (-) 1017 WP_241106062.1 pilus assembly protein PilP -
  MKK04_RS24745 (MKK04_24745) - 5381099..5381635 (-) 537 WP_233688150.1 PilN domain-containing protein -
  MKK04_RS24750 (MKK04_24750) pilM 5381632..5382513 (-) 882 WP_241106063.1 pilus assembly protein PilM -
  MKK04_RS24755 (MKK04_24755) - 5382705..5385158 (+) 2454 WP_207828275.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45845.63 Da        Isoelectric Point: 5.2143

>NTDB_id=660534 MKK04_RS24735 WP_233688152.1 5378894..5380162(-) (pilQ) [Pseudomonas sp. LS.1a]
MKTLKKWALAAVLPIPLALATPHQGEPLSLNFQDVEVRSLLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAEFAAQSVDARMGQALDAQLQPLRRELLPIHHANAGELAELLLASLADDGILTGRGSLSVDE
RTNTLVVYQPADRLAELRKLVAQLDVPVRQVAIEARIVEANVDYEKNLGVRWGGPLYVESPRPGKDLFVDLGVERVGSSI
GLGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNARVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMNDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=660534 MKK04_RS24735 WP_233688152.1 5378894..5380162(-) (pilQ) [Pseudomonas sp. LS.1a]
ATGAAAACATTGAAGAAATGGGCGCTGGCAGCGGTGCTGCCGATCCCGCTGGCGTTGGCGACGCCTCATCAGGGTGAACC
GCTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGCTGCTGCAGGTGCTGGCTGACTATGCCGGCGTCAATCTGGTCG
CCAGCGATGCCGTGCAGGGTAGCGTCACCTTGCGCTTGCAGGATGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGCGC
AGCAAGGGCCTGGCCCGGCGCCAGGAGGGTAATGTGCTGCTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAGTCTGTCGA
TGCCCGCATGGGGCAGGCGCTGGATGCGCAGTTGCAGCCGTTGCGTCGGGAACTGCTGCCCATCCATCACGCCAACGCCG
GCGAGCTGGCGGAGTTGCTGCTGGCGAGCCTGGCGGACGACGGTATTCTCACCGGGCGAGGTAGCCTGAGTGTCGATGAG
CGCACCAATACCCTGGTGGTATACCAGCCCGCCGACCGCCTTGCCGAGTTGCGGAAACTGGTGGCGCAGCTGGACGTGCC
GGTGCGCCAGGTGGCAATAGAGGCACGTATCGTCGAGGCCAACGTCGATTACGAGAAGAACCTGGGGGTGCGTTGGGGTG
GGCCGCTGTACGTTGAAAGCCCGCGGCCGGGCAAGGATTTGTTCGTCGACCTTGGAGTGGAGCGCGTGGGCAGCAGCATC
GGCCTGGGCCTGCTGCGTGGCGGCGTGCTGCTCGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGCAATGGCGAGATCAT
CTCGCAGCCCAAGGTCGTCACTGCCGACAAGGAAACGGCCAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCA
GCAAGAGTGGCGCCACCTCGGTCGCGTTCCGCGAAGCCTCGCTGTCGCTGGAGGTGACCCCGCAGATCACTCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAATGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAGGGTTCGCGTGGCGGACGGCGAAACGATCGTGATCGGTGGCGTGTACTCGACCTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAATGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.182

100

0.607
  pilQ Acinetobacter baumannii D1279779

41.126

100

0.45
  pilQ Acinetobacter baumannii strain A118

41.126

100

0.45
  comQ Acinetobacter baylyi ADP1

41.814

100

0.448
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.654

100

0.427
  pilQ Vibrio cholerae strain A1552

42.654

100

0.427
  pilQ Legionella pneumophila strain Lp02

41.587

98.578

0.41
  pilQ Legionella pneumophila strain ERS1305867

41.587

98.578

0.41
  pilQ Vibrio campbellii strain DS40M4

40.33

100

0.405
  pilQ Neisseria gonorrhoeae strain FA1090

37.41

98.815

0.37
  pilQ Neisseria meningitidis M1080

37.019

98.578

0.365
  pilQ Neisseria meningitidis MC58

37.019

98.578

0.365