Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   MKI56_RS10215 Genome accession   NZ_CP092574
Coordinates   2011666..2011941 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain VSE-WC032     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1967175..2030048 2011666..2011941 within 0


Gene organization within MGE regions


Location: 1967175..2030048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI56_RS09920 (MKI56_001966) - 1967175..1968182 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  MKI56_RS09925 (MKI56_001967) comGG 1968311..1968664 (-) 354 WP_002369174.1 competence type IV pilus minor pilin ComGG -
  MKI56_RS09930 (MKI56_001968) comGF 1968664..1969098 (-) 435 WP_002362055.1 competence type IV pilus minor pilin ComGF -
  MKI56_RS09935 (MKI56_001969) - 1969088..1969489 (-) 402 WP_014862199.1 type II secretion system protein -
  MKI56_RS09940 (MKI56_001970) hemH 1970211..1971152 (-) 942 WP_002364185.1 ferrochelatase -
  MKI56_RS09950 (MKI56_001972) - 1971663..1972853 (+) 1191 WP_002324630.1 IS256-like element ISLgar5 family transposase -
  MKI56_RS09955 (MKI56_001973) - 1973240..1973491 (-) 252 WP_002364188.1 hypothetical protein -
  MKI56_RS09960 (MKI56_001974) - 1973563..1973763 (-) 201 WP_002357053.1 cold-shock protein -
  MKI56_RS09965 (MKI56_001975) - 1974600..1975841 (-) 1242 WP_033781422.1 LysM peptidoglycan-binding domain-containing protein -
  MKI56_RS09970 (MKI56_001976) - 1975842..1976075 (-) 234 WP_002384371.1 phage holin -
  MKI56_RS09975 (MKI56_001977) - 1976068..1976289 (-) 222 WP_002364191.1 hypothetical protein -
  MKI56_RS09980 (MKI56_001978) - 1976324..1976479 (-) 156 WP_002364192.1 XkdX family protein -
  MKI56_RS09985 (MKI56_001979) - 1976481..1976801 (-) 321 WP_002389262.1 hypothetical protein -
  MKI56_RS09990 (MKI56_001980) - 1976815..1977306 (-) 492 WP_002364194.1 hypothetical protein -
  MKI56_RS09995 (MKI56_001981) - 1977306..1977593 (-) 288 WP_002357046.1 collagen-like protein -
  MKI56_RS10000 (MKI56_001982) - 1977590..1978186 (-) 597 WP_002357045.1 hypothetical protein -
  MKI56_RS10005 (MKI56_001983) - 1978179..1979060 (-) 882 WP_242427407.1 phage baseplate upper protein -
  MKI56_RS10010 (MKI56_001984) - 1979079..1981886 (-) 2808 WP_242427408.1 phage tail spike protein -
  MKI56_RS10015 (MKI56_001985) - 1981868..1982602 (-) 735 WP_242427409.1 phage tail protein -
  MKI56_RS10020 (MKI56_001986) - 1982592..1985489 (-) 2898 WP_002393612.1 tape measure protein -
  MKI56_RS10025 (MKI56_001987) - 1985737..1986087 (-) 351 WP_002357039.1 hypothetical protein -
  MKI56_RS10030 (MKI56_001988) - 1986140..1986988 (-) 849 WP_002384363.1 major tail protein -
  MKI56_RS10035 (MKI56_001989) - 1986989..1987363 (-) 375 WP_002357037.1 DUF6838 family protein -
  MKI56_RS10040 (MKI56_001990) - 1987366..1987764 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  MKI56_RS10045 (MKI56_001991) - 1987757..1988125 (-) 369 WP_002357034.1 hypothetical protein -
  MKI56_RS10050 (MKI56_001992) - 1988122..1988466 (-) 345 WP_002357033.1 hypothetical protein -
  MKI56_RS10055 (MKI56_001993) - 1988480..1988662 (-) 183 WP_033781426.1 hypothetical protein -
  MKI56_RS10060 (MKI56_001994) - 1988691..1989578 (-) 888 WP_002357030.1 DUF5309 domain-containing protein -
  MKI56_RS10065 (MKI56_001995) - 1989592..1990215 (-) 624 WP_002393977.1 DUF4355 domain-containing protein -
  MKI56_RS10070 (MKI56_001996) - 1990434..1990754 (-) 321 WP_002380436.1 hypothetical protein -
  MKI56_RS10075 (MKI56_001997) - 1990811..1991041 (-) 231 WP_002380437.1 hypothetical protein -
  MKI56_RS10080 (MKI56_001998) - 1991042..1992946 (-) 1905 WP_002393613.1 hypothetical protein -
  MKI56_RS10085 (MKI56_001999) - 1992921..1994408 (-) 1488 WP_002393614.1 phage portal protein -
  MKI56_RS10090 (MKI56_002000) - 1994420..1995709 (-) 1290 WP_002393615.1 PBSX family phage terminase large subunit -
  MKI56_RS10095 (MKI56_002001) terS 1995681..1996484 (-) 804 WP_002372038.1 phage terminase small subunit -
  MKI56_RS10100 (MKI56_002002) - 1997342..1997569 (-) 228 WP_002372042.1 hypothetical protein -
  MKI56_RS10110 (MKI56_002004) - 1999670..2000083 (-) 414 WP_242427410.1 ArpU family phage packaging/lysis transcriptional regulator -
  MKI56_RS10115 (MKI56_002005) - 2000989..2001423 (-) 435 WP_002372046.1 RusA family crossover junction endodeoxyribonuclease -
  MKI56_RS10120 (MKI56_002006) - 2001432..2001731 (-) 300 WP_002372047.1 MazG-like family protein -
  MKI56_RS10125 (MKI56_002007) - 2001732..2002034 (-) 303 WP_002368214.1 hypothetical protein -
  MKI56_RS10130 (MKI56_002008) - 2002038..2003000 (-) 963 WP_002372048.1 Lin1244/Lin1753 domain-containing protein -
  MKI56_RS10135 (MKI56_002009) - 2003014..2003904 (-) 891 WP_002364218.1 recombinase RecT -
  MKI56_RS10140 (MKI56_002010) - 2003907..2004849 (-) 943 Protein_1979 lambda-exonuclease family protein -
  MKI56_RS10145 (MKI56_002011) - 2004947..2005174 (-) 228 WP_002364220.1 hypothetical protein -
  MKI56_RS10150 (MKI56_002012) - 2005174..2005497 (-) 324 WP_002369793.1 hypothetical protein -
  MKI56_RS10155 (MKI56_002013) - 2005541..2005726 (-) 186 WP_002364222.1 hypothetical protein -
  MKI56_RS10160 (MKI56_002014) - 2005717..2005911 (-) 195 WP_002364223.1 hypothetical protein -
  MKI56_RS10165 (MKI56_002015) - 2005964..2006146 (+) 183 WP_002364224.1 YegP family protein -
  MKI56_RS10170 (MKI56_002016) - 2006186..2006908 (-) 723 WP_002364225.1 phage regulatory protein -
  MKI56_RS10175 (MKI56_002017) - 2006947..2007264 (-) 318 WP_341826380.1 hypothetical protein -
  MKI56_RS10180 (MKI56_002018) - 2007270..2007461 (-) 192 WP_002356998.1 hypothetical protein -
  MKI56_RS10185 (MKI56_002019) - 2007752..2008096 (+) 345 WP_002385819.1 helix-turn-helix transcriptional regulator -
  MKI56_RS10190 (MKI56_002020) - 2008101..2008749 (+) 649 Protein_1989 ImmA/IrrE family metallo-endopeptidase -
  MKI56_RS10195 (MKI56_002021) - 2008867..2009631 (+) 765 WP_002389030.1 LysM domain-containing protein -
  MKI56_RS10200 (MKI56_002022) - 2009646..2009975 (+) 330 WP_002369911.1 hypothetical protein -
  MKI56_RS10205 (MKI56_002023) - 2010050..2011198 (+) 1149 WP_002389015.1 site-specific integrase -
  MKI56_RS10210 (MKI56_002024) comGD 2011226..2011669 (-) 444 WP_002369912.1 competence type IV pilus minor pilin ComGD -
  MKI56_RS10215 (MKI56_002025) comGC/cglC 2011666..2011941 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  MKI56_RS10220 (MKI56_002026) comGB 2011941..2012987 (-) 1047 WP_002369171.1 competence type IV pilus assembly protein ComGB -
  MKI56_RS10225 (MKI56_002027) comGA 2012944..2013912 (-) 969 WP_002369168.1 competence type IV pilus ATPase ComGA -
  MKI56_RS10230 (MKI56_002028) - 2014154..2015482 (-) 1329 WP_242427412.1 amino acid permease -
  MKI56_RS10235 (MKI56_002029) rlmN 2015772..2016845 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MKI56_RS10240 (MKI56_002030) - 2016970..2018799 (-) 1830 WP_002401809.1 ABC transporter permease -
  MKI56_RS10245 (MKI56_002031) - 2018789..2019538 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  MKI56_RS10250 (MKI56_002032) - 2019656..2020357 (-) 702 WP_002356983.1 GntR family transcriptional regulator -
  MKI56_RS10255 (MKI56_002033) - 2020488..2022911 (-) 2424 WP_002369164.1 DNA translocase FtsK -
  MKI56_RS10260 (MKI56_002034) - 2023225..2024436 (-) 1212 WP_002360017.1 NAD(P)/FAD-dependent oxidoreductase -
  MKI56_RS10265 (MKI56_002035) - 2024465..2025370 (-) 906 WP_002360016.1 prenyltransferase -
  MKI56_RS10270 (MKI56_002036) - 2025492..2026472 (+) 981 WP_002369162.1 polyprenyl synthetase family protein -
  MKI56_RS10275 (MKI56_002037) cydC 2026552..2028318 (-) 1767 WP_202495308.1 thiol reductant ABC exporter subunit CydC -
  MKI56_RS10280 (MKI56_002038) cydD 2028315..2030048 (-) 1734 WP_002364369.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=659075 MKI56_RS10215 WP_002356991.1 2011666..2011941(-) (comGC/cglC) [Enterococcus faecalis strain VSE-WC032]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=659075 MKI56_RS10215 WP_002356991.1 2011666..2011941(-) (comGC/cglC) [Enterococcus faecalis strain VSE-WC032]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396