Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   L2629_RS00750 Genome accession   NZ_CP091198
Coordinates   35789..36064 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain UK045     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1598..54171 35789..36064 within 0


Gene organization within MGE regions


Location: 1598..54171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2629_RS00510 (L2629_00505) - 1598..2479 (-) 882 WP_002357044.1 phage baseplate upper protein -
  L2629_RS00515 (L2629_00510) - 2498..5305 (-) 2808 WP_236334911.1 phage tail spike protein -
  L2629_RS00520 (L2629_00515) - 5287..6021 (-) 735 WP_002403868.1 tail protein -
  L2629_RS00525 (L2629_00520) - 6011..8908 (-) 2898 WP_236335466.1 tape measure protein -
  L2629_RS00530 (L2629_00525) gpG 9128..9504 (-) 377 Protein_8 phage tail assembly chaperone G -
  L2629_RS00535 (L2629_00530) - 9557..10404 (-) 848 Protein_9 major tail protein -
  L2629_RS00545 (L2629_00540) - 10405..10779 (-) 375 WP_002387286.1 DUF6838 family protein -
  L2629_RS00550 (L2629_00545) - 10782..11180 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  L2629_RS00555 (L2629_00550) - 11173..11547 (-) 375 WP_002387285.1 hypothetical protein -
  L2629_RS00560 (L2629_00555) - 11544..11843 (-) 300 WP_070702965.1 hypothetical protein -
  L2629_RS00565 (L2629_00560) - 11901..12083 (-) 183 WP_002387281.1 hypothetical protein -
  L2629_RS00570 (L2629_00565) - 12112..12999 (-) 888 WP_002387280.1 DUF5309 domain-containing protein -
  L2629_RS00575 (L2629_00570) - 13013..13636 (-) 624 WP_010706493.1 DUF4355 domain-containing protein -
  L2629_RS00580 (L2629_00575) - 13805..14125 (-) 321 WP_002380436.1 hypothetical protein -
  L2629_RS00585 (L2629_00580) - 14182..14412 (-) 231 WP_002380437.1 hypothetical protein -
  L2629_RS00590 (L2629_00585) - 14413..16317 (-) 1905 WP_010706494.1 head protein -
  L2629_RS00595 (L2629_00590) - 16292..17764 (-) 1473 WP_010706495.1 phage portal protein -
  L2629_RS00600 (L2629_00595) terL 17776..19164 (-) 1389 WP_002387276.1 phage terminase large subunit -
  L2629_RS00605 (L2629_00600) - 19157..19618 (-) 462 WP_002383816.1 helix-turn-helix domain-containing protein -
  L2629_RS00610 (L2629_00605) - 19694..19888 (-) 195 WP_002383815.1 hypothetical protein -
  L2629_RS00615 (L2629_00610) - 20054..20866 (-) 813 WP_002387275.1 hypothetical protein -
  L2629_RS00620 (L2629_00615) - 20978..21211 (-) 234 Protein_25 hypothetical protein -
  L2629_RS00625 (L2629_00620) - 21795..22265 (-) 471 WP_002383811.1 hypothetical protein -
  L2629_RS00630 (L2629_00625) - 22290..23213 (-) 924 WP_002383810.1 DUF6731 family protein -
  L2629_RS00640 (L2629_00635) - 23931..24347 (-) 417 WP_010706497.1 ArpU family phage packaging/lysis transcriptional regulator -
  L2629_RS00645 - 24715..24975 (-) 261 WP_002383807.1 hypothetical protein -
  L2629_RS00650 (L2629_00640) - 25255..25663 (-) 409 Protein_30 RusA family crossover junction endodeoxyribonuclease -
  L2629_RS00655 (L2629_00645) - 25660..26178 (-) 519 Protein_31 helix-turn-helix domain-containing protein -
  L2629_RS00660 (L2629_00650) - 26277..27448 (+) 1172 Protein_32 IS256-like element IS256 family transposase -
  L2629_RS00665 (L2629_00655) - 27501..27848 (-) 348 Protein_33 helix-turn-helix domain-containing protein -
  L2629_RS00670 (L2629_00660) - 27848..28048 (-) 201 WP_002357010.1 hypothetical protein -
  L2629_RS00675 (L2629_00665) - 28053..28436 (-) 384 WP_002383802.1 putative HNHc nuclease -
  L2629_RS00680 (L2629_00670) - 28475..28693 (-) 219 WP_002383801.1 hypothetical protein -
  L2629_RS00685 (L2629_00675) - 28698..29432 (-) 735 WP_002383799.1 ERF family protein -
  L2629_RS00690 (L2629_00680) - 29425..29742 (-) 318 WP_236335180.1 hypothetical protein -
  L2629_RS00695 (L2629_00685) - 29938..30276 (-) 339 WP_002383796.1 hypothetical protein -
  L2629_RS00700 (L2629_00690) - 30313..30522 (-) 210 WP_002378465.1 hypothetical protein -
  L2629_RS00705 (L2629_00695) - 30577..30765 (+) 189 WP_002357001.1 YegP family protein -
  L2629_RS00710 (L2629_00700) - 30791..31513 (-) 723 WP_002387270.1 phage regulatory protein -
  L2629_RS00715 (L2629_00705) - 31536..31847 (-) 312 WP_002403885.1 hypothetical protein -
  L2629_RS00720 (L2629_00710) - 31859..32050 (-) 192 WP_002383936.1 hypothetical protein -
  L2629_RS00725 (L2629_00715) - 32346..32687 (+) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  L2629_RS00730 (L2629_00720) - 32692..33342 (+) 651 WP_002387266.1 ImmA/IrrE family metallo-endopeptidase -
  L2629_RS00735 (L2629_00725) - 33438..34067 (+) 630 WP_002387265.1 SHOCT domain-containing protein -
  L2629_RS00740 (L2629_00730) - 34173..35321 (+) 1149 WP_002387264.1 site-specific integrase -
  L2629_RS00745 (L2629_00735) comGD 35358..35792 (-) 435 Protein_49 competence type IV pilus minor pilin ComGD -
  L2629_RS00750 (L2629_00740) comGC/cglC 35789..36064 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  L2629_RS00755 (L2629_00745) comGB 36064..37110 (-) 1047 WP_002356990.1 competence type IV pilus assembly protein ComGB -
  L2629_RS00760 (L2629_00750) comGA 37067..38035 (-) 969 WP_002364362.1 competence type IV pilus ATPase ComGA -
  L2629_RS00765 (L2629_00755) - 38276..39604 (-) 1329 WP_002362058.1 APC family permease -
  L2629_RS00770 (L2629_00760) rlmN 39895..40968 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  L2629_RS00775 (L2629_00765) - 41093..42923 (-) 1831 Protein_55 ABC transporter permease -
  L2629_RS00780 (L2629_00770) - 42913..43662 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  L2629_RS00785 (L2629_00775) - 43780..44481 (-) 702 WP_002364244.1 GntR family transcriptional regulator -
  L2629_RS00790 (L2629_00780) - 44612..47035 (-) 2424 WP_002364367.1 DNA translocase FtsK -
  L2629_RS00795 (L2629_00785) - 47349..48560 (-) 1212 WP_002360017.1 NAD(P)/FAD-dependent oxidoreductase -
  L2629_RS00800 (L2629_00790) - 48589..49494 (-) 906 WP_002387262.1 prenyltransferase -
  L2629_RS00805 (L2629_00795) - 49616..50596 (+) 981 WP_010706503.1 polyprenyl synthetase family protein -
  L2629_RS00810 (L2629_00800) cydC 50676..52442 (-) 1767 WP_002383727.1 thiol reductant ABC exporter subunit CydC -
  L2629_RS00815 (L2629_00805) cydD 52439..54171 (-) 1733 Protein_63 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=648264 L2629_RS00750 WP_002356991.1 35789..36064(-) (comGC/cglC) [Enterococcus faecalis strain UK045]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=648264 L2629_RS00750 WP_002356991.1 35789..36064(-) (comGC/cglC) [Enterococcus faecalis strain UK045]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396