Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   X970_RS01575 Genome accession   NC_023076
Coordinates   328093..328503 (+) Length   136 a.a.
NCBI ID   WP_013970772.1    Uniprot ID   -
Organism   Pseudomonas monteilii SB3101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323093..333503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X970_RS01545 (X970_01570) - 323619..324050 (-) 432 WP_015268860.1 hypothetical protein -
  X970_RS01550 (X970_01575) - 324137..324844 (+) 708 WP_044039496.1 energy-coupling factor ABC transporter permease -
  X970_RS01555 (X970_01580) yacG 324980..325180 (-) 201 WP_003260624.1 DNA gyrase inhibitor YacG -
  X970_RS01560 (X970_01585) coaE 325177..325800 (-) 624 WP_024086216.1 dephospho-CoA kinase -
  X970_RS01565 (X970_01590) pilD 325800..326666 (-) 867 WP_024086217.1 prepilin peptidase Machinery gene
  X970_RS01570 (X970_01595) - 326669..327877 (-) 1209 WP_024086218.1 type II secretion system F family protein -
  X970_RS01575 (X970_01600) pilA 328093..328503 (+) 411 WP_013970772.1 pilin Machinery gene
  X970_RS29005 - 328814..328948 (+) 135 Protein_300 integrase -
  X970_RS01585 (X970_01610) - 329232..329810 (+) 579 WP_230491488.1 toll/interleukin-1 receptor domain-containing protein -
  X970_RS01590 (X970_01615) - 329831..330322 (+) 492 WP_024086220.1 TIR domain-containing protein -
  X970_RS01595 (X970_01620) - 330387..331811 (+) 1425 WP_024086221.1 SIR2 family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13769.02 Da        Isoelectric Point: 7.0812

>NTDB_id=64260 X970_RS01575 WP_013970772.1 328093..328503(+) (pilA) [Pseudomonas monteilii SB3101]
MKGQRGITLIELMIVVAIIGILATIALPMYTNHQSRSKAAAGLLEISALKTAMDLRLNDGKDVPDVAALGGQPATAHCAI
TASGKAADGTGSIVCTLVDAPANALGKALTLTRSASGWGCTTDIEEDLAPKGCKAP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=64260 X970_RS01575 WP_013970772.1 328093..328503(+) (pilA) [Pseudomonas monteilii SB3101]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTT
ACCGATGTACACCAACCACCAGTCACGCTCCAAGGCCGCGGCCGGGTTGCTGGAGATCAGCGCGCTGAAGACCGCGATGG
ACCTGCGGTTGAATGATGGGAAGGATGTGCCGGATGTGGCCGCGCTGGGTGGCCAGCCGGCGACGGCGCACTGTGCGATC
ACGGCTAGCGGCAAGGCGGCGGATGGCACTGGGAGCATCGTTTGCACGCTGGTGGATGCGCCGGCCAATGCGCTGGGCAA
GGCATTGACCCTGACGCGTTCGGCTAGCGGGTGGGGGTGCACGACCGACATTGAAGAAGACCTGGCGCCGAAGGGGTGCA
AGGCGCCTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.485
  pilA Acinetobacter baumannii strain A118

44.604

100

0.456
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.434
  comP Acinetobacter baylyi ADP1

39.456

100

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.963

99.265

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.647

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.063

100

0.39
  pilA Vibrio cholerae strain A1552

37.063

100

0.39
  pilA Vibrio cholerae C6706

37.063

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

38.462

95.588

0.368

Multiple sequence alignment