Detailed information NTG0064259

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: X970_RS01565
• Genome accession: NC_023076
• Coordinates: 325800..326666 (-)
• Length: 288 a.a.
• NCBI ID: WP_024086217.1
• Uniprot ID: V9UUM7
• Organism: Pseudomonas monteilii SB3101
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 320800..331666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
X970_RS01530 (X970_01555)	-	321247..322542 (-)	1296	WP_003260618.1	NAD(P)/FAD-dependent oxidoreductase	-
X970_RS01535 (X970_01560)	-	322627..322800 (-)	174	WP_013970765.1	DUF3094 family protein	-
X970_RS01540 (X970_01565)	-	322934..323563 (-)	630	WP_013970766.1	DUF1780 domain-containing protein	-
X970_RS01545 (X970_01570)	-	323619..324050 (-)	432	WP_015268860.1	hypothetical protein	-
X970_RS01550 (X970_01575)	-	324137..324844 (+)	708	WP_044039496.1	energy-coupling factor ABC transporter permease	-
X970_RS01555 (X970_01580)	yacG	324980..325180 (-)	201	WP_003260624.1	DNA gyrase inhibitor YacG	-
X970_RS01560 (X970_01585)	coaE	325177..325800 (-)	624	WP_024086216.1	dephospho-CoA kinase	-
X970_RS01565 (X970_01590)	pilD	325800..326666 (-)	867	WP_024086217.1	prepilin peptidase	Machinery gene
X970_RS01570 (X970_01595)	-	326669..327877 (-)	1209	WP_024086218.1	type II secretion system F family protein	-
X970_RS01575 (X970_01600)	pilA	328093..328503 (+)	411	WP_013970772.1	pilin	Machinery gene
X970_RS29005	-	328814..328948 (+)	135	Protein_300	integrase	-
X970_RS01585 (X970_01610)	-	329232..329810 (+)	579	WP_230491488.1	toll/interleukin-1 receptor domain-containing protein	-
X970_RS01590 (X970_01615)	-	329831..330322 (+)	492	WP_024086220.1	TIR domain-containing protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32020.19 Da
• Isoelectric Point: 8.2287

>NTDB_id=64259 X970_RS01565 WP_024086217.1 325800..326666(-) (pilD) [Pseudomonas monteilii SB3101]
MTLWTLLAEQPAYFFTLAGVLGLLVGSFLNVLVYRLPIMLERQWQREAQEMLQLPITPHERFDLWLPASRCPHCAHRIRA
WENIPVISYLALRGRCSACKTRISPRYPLVELASALLSLVVAWRFGASPEALLALPLTWCLLALSLIDAEHQLLPDALVL
PMLWLGLIVNAFGLYVPLTSALWGAVAGYLSLWTVYWVFKLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LAGLCLLRLRRHAMGTAIPFGPYLAIAGWIAVLWGDEIYASYTNLLGF

Nucleotide

• Length: 867 bp

>NTDB_id=64259 X970_RS01565 WP_024086217.1 325800..326666(-) (pilD) [Pseudomonas monteilii SB3101]
ATGACTTTATGGACTTTGCTGGCTGAGCAGCCGGCGTACTTCTTCACCCTGGCCGGCGTGCTCGGCCTGCTGGTGGGCAG
TTTTCTCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAGGAAATGCTGCAGT
TGCCGATCACACCGCACGAGCGTTTCGACCTGTGGCTACCCGCCTCGCGCTGCCCGCATTGCGCGCATCGGATTCGCGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGCGCGGGCGTTGTTCAGCCTGCAAAACCCGCATCAGCCCGCGCTA
CCCATTGGTGGAGTTAGCCAGCGCGCTGCTGTCGCTGGTGGTGGCCTGGCGCTTTGGTGCTTCGCCCGAGGCCTTGCTGG
CGCTGCCGCTGACCTGGTGCCTGCTGGCCCTGAGCCTGATCGATGCCGAGCACCAGTTGCTGCCGGATGCGCTGGTGCTG
CCGATGCTGTGGCTGGGGCTGATCGTCAACGCCTTTGGCCTCTACGTCCCGCTGACCAGTGCGTTGTGGGGCGCGGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAAGCTGGTTACCGGCAAGGAGGGCATGGGCTATGGCGACTTCA
AGCTGATGGCGCTGATCGGGGCCTGGGGTGGCTGGCAGGTGTTGCCGTTGACCTTGCTGTTGTCGTCAGTGGTCGGGGCG
CTGGCTGGGCTGTGCCTGTTGCGCTTGCGCCGGCATGCGATGGGCACGGCGATCCCGTTTGGCCCCTATCTGGCGATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACACAAACCTGCTTGGATTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: V9UUM7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.182	95.486	0.517
pilD	Acinetobacter baumannii D1279779	52.222	93.75	0.49
pilD	Vibrio campbellii strain DS40M4	49.822	97.569	0.486
pilD	Acinetobacter nosocomialis M2	51.481	93.75	0.483
pilD	Neisseria gonorrhoeae MS11	51.698	92.014	0.476