Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   LRS65_RS10560 Genome accession   NZ_CP089601
Coordinates   2026734..2027069 (+) Length   111 a.a.
NCBI ID   WP_000982018.1    Uniprot ID   A0A9W5VT75
Organism   Bacillus cereus strain C-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1964845..2027069 2026734..2027069 within 0


Gene organization within MGE regions


Location: 1964845..2027069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRS65_RS10205 (LRS65_10185) - 1964845..1965981 (-) 1137 WP_257116024.1 site-specific integrase -
  LRS65_RS10210 (LRS65_10190) - 1966002..1966520 (-) 519 WP_098656329.1 ImmA/IrrE family metallo-endopeptidase -
  LRS65_RS10215 (LRS65_10195) - 1967201..1967809 (-) 609 WP_000670775.1 hypothetical protein -
  LRS65_RS10220 (LRS65_10200) - 1967806..1968399 (-) 594 WP_000967383.1 hypothetical protein -
  LRS65_RS10225 (LRS65_10205) - 1968368..1969537 (-) 1170 WP_000433590.1 hypothetical protein -
  LRS65_RS10230 (LRS65_10210) - 1969696..1970067 (-) 372 WP_000105316.1 helix-turn-helix domain-containing protein -
  LRS65_RS10235 (LRS65_10215) - 1970224..1970481 (+) 258 WP_000598018.1 helix-turn-helix transcriptional regulator -
  LRS65_RS10240 (LRS65_10220) - 1970490..1970789 (+) 300 WP_215571507.1 helix-turn-helix domain-containing protein -
  LRS65_RS10245 (LRS65_10225) - 1970857..1971372 (+) 516 WP_257116025.1 helix-turn-helix transcriptional regulator -
  LRS65_RS10250 (LRS65_10230) - 1971403..1971624 (+) 222 WP_000151152.1 hypothetical protein -
  LRS65_RS10255 (LRS65_10235) - 1971803..1972087 (+) 285 WP_137050449.1 hypothetical protein -
  LRS65_RS10260 (LRS65_10240) - 1972205..1973182 (+) 978 WP_257116026.1 DnaD domain protein -
  LRS65_RS10265 (LRS65_10245) - 1973193..1973555 (+) 363 WP_119683270.1 cell division protein SepF -
  LRS65_RS10270 (LRS65_10250) - 1973627..1973818 (+) 192 WP_257116027.1 DUF3954 domain-containing protein -
  LRS65_RS10275 (LRS65_10255) - 1973900..1974574 (-) 675 WP_257116028.1 collagen-like protein -
  LRS65_RS10280 (LRS65_10260) - 1975128..1975994 (-) 867 WP_119683271.1 exosporium protein D -
  LRS65_RS10285 (LRS65_10265) - 1976290..1977045 (-) 756 WP_257116029.1 exosporium leader peptide-containing protein -
  LRS65_RS10290 (LRS65_10270) - 1977326..1977790 (-) 465 WP_002163978.1 hypothetical protein -
  LRS65_RS10295 (LRS65_10275) - 1978755..1979150 (+) 396 WP_257116030.1 hypothetical protein -
  LRS65_RS10300 (LRS65_10280) - 1980747..1980908 (+) 162 WP_162900585.1 hypothetical protein -
  LRS65_RS10305 (LRS65_10285) - 1980936..1981418 (+) 483 WP_119683275.1 ArpU family phage packaging/lysis transcriptional regulator -
  LRS65_RS10310 (LRS65_10290) - 1981418..1981960 (+) 543 WP_119683276.1 site-specific integrase -
  LRS65_RS10315 (LRS65_10295) - 1982326..1983996 (+) 1671 WP_240440586.1 sialidase family protein -
  LRS65_RS10320 (LRS65_10300) - 1984388..1984606 (+) 219 WP_000930975.1 hypothetical protein -
  LRS65_RS10325 (LRS65_10305) - 1985032..1985238 (-) 207 WP_001068928.1 hypothetical protein -
  LRS65_RS10330 (LRS65_10310) - 1985507..1985911 (+) 405 WP_063536219.1 hypothetical protein -
  LRS65_RS10335 (LRS65_10315) - 1985911..1986126 (+) 216 WP_063536220.1 hypothetical protein -
  LRS65_RS10340 (LRS65_10320) - 1986286..1986858 (+) 573 WP_000576168.1 recombinase family protein -
  LRS65_RS10345 (LRS65_10325) - 1986910..1987089 (+) 180 WP_257116031.1 hypothetical protein -
  LRS65_RS10350 (LRS65_10330) terS 1987111..1987959 (+) 849 WP_078420678.1 phage terminase small subunit -
  LRS65_RS10355 (LRS65_10335) - 1987952..1989358 (+) 1407 WP_078420677.1 DEAD/DEAH box helicase family protein -
  LRS65_RS10360 (LRS65_10340) - 1989428..1990903 (+) 1476 WP_257116032.1 phage portal protein -
  LRS65_RS10365 (LRS65_10345) - 1991017..1991535 (+) 519 WP_257116033.1 phage head morphogenesis protein -
  LRS65_RS10370 (LRS65_10350) - 1991648..1992850 (+) 1203 WP_257116034.1 XkdF-like putative serine protease domain-containing protein -
  LRS65_RS10375 (LRS65_10355) - 1992880..1993857 (+) 978 WP_106825134.1 phage major capsid protein -
  LRS65_RS10380 (LRS65_10360) - 1994028..1994192 (+) 165 WP_000869888.1 YqbF domain-containing protein -
  LRS65_RS10385 (LRS65_10365) - 1994192..1994728 (+) 537 WP_257116035.1 hypothetical protein -
  LRS65_RS10390 (LRS65_10370) - 1994725..1995096 (+) 372 WP_257116036.1 hypothetical protein -
  LRS65_RS10395 (LRS65_10375) - 1995103..1995513 (+) 411 WP_000024034.1 HK97-gp10 family putative phage morphogenesis protein -
  LRS65_RS10400 (LRS65_10380) - 1995517..1995939 (+) 423 WP_000063397.1 hypothetical protein -
  LRS65_RS10405 (LRS65_10385) - 1995952..1996554 (+) 603 WP_000118571.1 hypothetical protein -
  LRS65_RS10410 (LRS65_10390) - 1996612..1997142 (+) 531 WP_000146557.1 hypothetical protein -
  LRS65_RS10415 (LRS65_10395) - 1997094..1997537 (+) 444 WP_257116037.1 hypothetical protein -
  LRS65_RS10420 (LRS65_10400) - 1997572..1998504 (+) 933 Protein_1977 phage tail tape measure protein -
  LRS65_RS10425 (LRS65_10405) - 1998935..1999639 (+) 705 WP_257116039.1 hypothetical protein -
  LRS65_RS10430 (LRS65_10410) - 1999816..2001822 (+) 2007 WP_345893699.1 hypothetical protein -
  LRS65_RS10435 (LRS65_10415) - 2001819..2002502 (+) 684 WP_257116041.1 hypothetical protein -
  LRS65_RS10440 (LRS65_10420) - 2002499..2004046 (+) 1548 WP_257116042.1 hypothetical protein -
  LRS65_RS10445 (LRS65_10425) - 2004057..2004440 (+) 384 WP_021727719.1 hypothetical protein -
  LRS65_RS10450 (LRS65_10430) - 2004485..2004859 (+) 375 WP_257116043.1 Low copy number virion structural protein -
  LRS65_RS10455 (LRS65_10435) - 2004872..2005249 (+) 378 WP_017673856.1 hypothetical protein -
  LRS65_RS10460 (LRS65_10440) - 2005263..2006600 (+) 1338 WP_257116044.1 hypothetical protein -
  LRS65_RS10465 (LRS65_10445) - 2006614..2008341 (+) 1728 WP_061885289.1 hypothetical protein -
  LRS65_RS10470 (LRS65_10450) - 2008365..2010590 (+) 2226 WP_257116045.1 hypothetical protein -
  LRS65_RS10475 (LRS65_10455) - 2010572..2012077 (+) 1506 WP_235598831.1 cell adhesion protein -
  LRS65_RS10480 (LRS65_10460) - 2012087..2012470 (+) 384 WP_061457126.1 hypothetical protein -
  LRS65_RS10485 (LRS65_10465) - 2012484..2013404 (+) 921 WP_061457127.1 hypothetical protein -
  LRS65_RS10490 (LRS65_10470) - 2013713..2014210 (+) 498 WP_000439586.1 phage holin family protein -
  LRS65_RS10495 (LRS65_10475) - 2014289..2015347 (+) 1059 WP_061457128.1 N-acetylmuramoyl-L-alanine amidase -
  LRS65_RS10500 (LRS65_10480) - 2015405..2015542 (-) 138 WP_170879373.1 hypothetical protein -
  LRS65_RS10505 (LRS65_10485) - 2015544..2016632 (-) 1089 WP_061457129.1 hypothetical protein -
  LRS65_RS10510 (LRS65_10490) - 2017411..2017626 (+) 216 WP_061457132.1 hypothetical protein -
  LRS65_RS10515 (LRS65_10495) - 2017720..2018010 (+) 291 WP_061457133.1 transposase -
  LRS65_RS10520 (LRS65_10500) - 2018431..2018751 (-) 321 Protein_1997 transposase -
  LRS65_RS10525 (LRS65_10505) - 2019693..2020982 (+) 1290 WP_257116046.1 exosporium glycoprotein BclB-related protein -
  LRS65_RS10530 (LRS65_10510) - 2021254..2021388 (+) 135 Protein_1999 ArpU family transcriptional regulator -
  LRS65_RS10535 (LRS65_10515) - 2021929..2022756 (-) 828 WP_025709741.1 hypothetical protein -
  LRS65_RS10540 (LRS65_10520) - 2022866..2023513 (+) 648 WP_000165965.1 HD domain-containing protein -
  LRS65_RS10545 (LRS65_10525) - 2023510..2025405 (+) 1896 WP_257116047.1 ABC-F family ATP-binding cassette domain-containing protein -
  LRS65_RS10550 (LRS65_10530) - 2025481..2026212 (+) 732 WP_025687369.1 Bax inhibitor-1/YccA family protein -
  LRS65_RS10555 (LRS65_10535) - 2026318..2026572 (+) 255 WP_000975137.1 DUF4318 domain-containing protein -
  LRS65_RS10560 (LRS65_10540) ssbA 2026734..2027069 (+) 336 WP_000982018.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 111 a.a.        Molecular weight: 12771.61 Da        Isoelectric Point: 9.4344

>NTDB_id=638307 LRS65_RS10560 WP_000982018.1 2026734..2027069(+) (ssbA) [Bacillus cereus strain C-1]
MMNRVVLIGRLTKEPELYYTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCKKGSLVGITGRIQTS
NYDDEQGKRIYRTEVVIESITFLERRREGAS

Nucleotide


Download         Length: 336 bp        

>NTDB_id=638307 LRS65_RS10560 WP_000982018.1 2026734..2027069(+) (ssbA) [Bacillus cereus strain C-1]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAACAAGGCGTCGCTTATGC
ACGAATATGTGTTGCGGTGAATAGAGGATTTCGAAATAGTTTAGGTGAACAACAAGTCGATTTTATTAATTGTGTCGTTT
GGCGCAAATCGGCTGAGAATGTAACTGAATATTGTAAAAAGGGATCACTCGTTGGGATTACAGGGCGTATTCAGACTAGT
AATTACGATGATGAACAAGGCAAGAGAATATATAGAACTGAAGTTGTGATTGAGAGTATTACCTTTTTGGAGAGAAGGCG
GGAGGGGGCATCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.491

95.495

0.559

  ssb Latilactobacillus sakei subsp. sakei 23K

55.66

95.495

0.532

  ssbB Bacillus subtilis subsp. subtilis str. 168

50.893

100

0.514

  ssbB Lactococcus lactis subsp. cremoris KW2

45.714

94.595

0.432

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.636

99.099

0.432

  ssbA Streptococcus mutans UA159

40

99.099

0.396

  ssbB/cilA Streptococcus mitis NCTC 12261

40

99.099

0.396

  ssbB/cilA Streptococcus pneumoniae TIGR4

40

99.099

0.396

  ssbB/cilA Streptococcus pneumoniae D39

39.091

99.099

0.387

  ssbB/cilA Streptococcus pneumoniae R6

39.091

99.099

0.387

  ssbB/cilA Streptococcus mitis SK321

39.091

99.099

0.387

  ssbB/cilA Streptococcus pneumoniae Rx1

39.091

99.099

0.387