Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LRK32_RS04610 Genome accession   NZ_CP088920
Coordinates   1035519..1035959 (-) Length   146 a.a.
NCBI ID   WP_027490743.1    Uniprot ID   -
Organism   Rhodanobacter denitrificans strain FW104-10F02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030519..1040959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK32_RS04600 (LRK32_04600) - 1031476..1033440 (-) 1965 WP_027490745.1 hypothetical protein -
  LRK32_RS04605 (LRK32_04605) - 1033440..1035452 (-) 2013 WP_027490744.1 hypothetical protein -
  LRK32_RS04610 (LRK32_04610) pilE 1035519..1035959 (-) 441 WP_027490743.1 pilin Machinery gene
  LRK32_RS04615 (LRK32_04615) - 1036302..1036745 (-) 444 WP_027490742.1 pilin -
  LRK32_RS04620 (LRK32_04620) - 1036936..1039065 (+) 2130 WP_027490741.1 S9 family peptidase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15347.84 Da        Isoelectric Point: 10.0547

>NTDB_id=633950 LRK32_RS04610 WP_027490743.1 1035519..1035959(-) (pilE) [Rhodanobacter denitrificans strain FW104-10F02]
MKKMQKGFTLIELMIVVAIIAILAAIAIPAYQDYLIRTQVSEGAVLTDGAKTAVAEFYSNRGALTNAKNASIGLAQSQSI
NGQYVSKVEVVGGVITASFGGPKANVTALPSSKTFVLSPITQSGSIKWTCTGSTVDQKYLPTSCRK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=633950 LRK32_RS04610 WP_027490743.1 1035519..1035959(-) (pilE) [Rhodanobacter denitrificans strain FW104-10F02]
ATGAAGAAGATGCAGAAGGGCTTCACCCTGATCGAACTGATGATCGTGGTCGCGATCATCGCGATCCTCGCCGCGATCGC
CATCCCTGCCTACCAGGATTACCTGATCCGTACGCAGGTGTCCGAGGGCGCGGTGCTGACCGACGGCGCCAAGACGGCCG
TTGCCGAGTTCTACTCGAACCGCGGCGCCCTGACCAATGCCAAGAACGCCAGTATCGGTTTGGCGCAATCCCAAAGTATC
AACGGACAGTACGTCAGCAAAGTTGAGGTTGTGGGTGGCGTCATCACTGCCTCGTTCGGTGGCCCGAAGGCGAACGTGAC
AGCCCTGCCCAGCTCCAAGACTTTCGTGCTCTCCCCCATCACCCAGTCCGGGTCGATCAAGTGGACCTGCACTGGGTCGA
CGGTCGATCAGAAGTACCTGCCAACTTCGTGCCGCAAGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(37-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

45.342

100

0.5
  pilE Neisseria gonorrhoeae MS11

43.21

100

0.479
  pilA2 Legionella pneumophila strain ERS1305867

47.917

98.63

0.473
  pilA2 Legionella pneumophila str. Paris

48.252

97.945

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

40.828

100

0.473
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.737

100

0.452
  pilA/pilA1 Eikenella corrodens VA1

40.506

100

0.438
  comP Acinetobacter baylyi ADP1

43.537

100

0.438
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.255

100

0.39
  pilA Acinetobacter baumannii strain A118

37.931

99.315

0.377
  pilA Pseudomonas aeruginosa PAK

34.615

100

0.37