Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LRK24_RS14880 Genome accession   NZ_CP088919
Coordinates   3225744..3226175 (+) Length   143 a.a.
NCBI ID   WP_007513901.1    Uniprot ID   -
Organism   Rhodanobacter denitrificans strain DSM-24678     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3222496..3247378 3225744..3226175 within 0


Gene organization within MGE regions


Location: 3222496..3247378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK24_RS14865 (LRK24_14865) - 3222956..3223312 (+) 357 WP_202946748.1 GtrA family protein -
  LRK24_RS14870 (LRK24_14870) - 3223452..3224822 (+) 1371 WP_007513896.1 glycosyltransferase family 39 protein -
  LRK24_RS14875 (LRK24_14875) - 3224958..3225401 (+) 444 WP_007513899.1 pilin -
  LRK24_RS14880 (LRK24_14880) pilE 3225744..3226175 (+) 432 WP_007513901.1 pilin Machinery gene
  LRK24_RS14885 (LRK24_14885) - 3226246..3228255 (+) 2010 WP_007513903.1 hypothetical protein -
  LRK24_RS14890 (LRK24_14890) - 3228255..3230225 (+) 1971 WP_043292834.1 hypothetical protein -
  LRK24_RS14895 (LRK24_14895) - 3230264..3231385 (+) 1122 WP_007513907.1 glycosyltransferase -
  LRK24_RS14900 (LRK24_14900) - 3231378..3232304 (+) 927 WP_043292835.1 NAD-dependent epimerase/dehydratase family protein -
  LRK24_RS14905 (LRK24_14905) - 3232274..3233320 (+) 1047 WP_007513911.1 glycosyltransferase -
  LRK24_RS14910 (LRK24_14910) - 3233412..3234563 (-) 1152 WP_007513913.1 glycoside hydrolase family 99-like domain-containing protein -
  LRK24_RS14915 (LRK24_14915) - 3234563..3235816 (-) 1254 WP_007513915.1 class I SAM-dependent methyltransferase -
  LRK24_RS14920 (LRK24_14920) - 3235813..3236772 (-) 960 WP_007513916.1 GDP-mannose 4,6-dehydratase -
  LRK24_RS14925 (LRK24_14925) - 3236772..3237797 (-) 1026 WP_007513917.1 glycosyltransferase family 2 protein -
  LRK24_RS14930 (LRK24_14930) - 3237797..3238648 (-) 852 WP_007513918.1 hypothetical protein -
  LRK24_RS14935 (LRK24_14935) - 3238664..3240172 (-) 1509 WP_007513919.1 oligosaccharide flippase family protein -
  LRK24_RS14940 (LRK24_14940) gmd 3240177..3241271 (-) 1095 WP_043292836.1 GDP-mannose 4,6-dehydratase -
  LRK24_RS14945 (LRK24_14945) - 3241315..3242172 (-) 858 WP_007513921.1 glycosyltransferase family 2 protein -
  LRK24_RS14950 (LRK24_14950) - 3242464..3242859 (-) 396 WP_007513922.1 type II toxin-antitoxin system VapC family toxin -
  LRK24_RS14955 (LRK24_14955) - 3242856..3243098 (-) 243 WP_007513924.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  LRK24_RS14960 (LRK24_14960) pilB 3243698..3245422 (+) 1725 WP_007513926.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LRK24_RS14965 (LRK24_14965) pilC 3245567..3246838 (+) 1272 WP_007513928.1 type II secretion system F family protein Machinery gene
  LRK24_RS14970 (LRK24_14970) - 3246986..3247378 (+) 393 WP_007513930.1 DUF559 domain-containing protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14917.27 Da        Isoelectric Point: 9.8639

>NTDB_id=633929 LRK24_RS14880 WP_007513901.1 3225744..3226175(+) (pilE) [Rhodanobacter denitrificans strain DSM-24678]
MKNVQKGFTLIELMIVVAIIAILAAIAIPAYQDYLIRTQVSEGAVLTDGAKTAISEFYSNTGSFPSKNASAGLASPASIT
GKYVAKVDVSATTGQIVATFGKDANKAIKTSTFVLSAITSTGSIAWSCKKSTVDPKYLPTSCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=633929 LRK24_RS14880 WP_007513901.1 3225744..3226175(+) (pilE) [Rhodanobacter denitrificans strain DSM-24678]
ATGAAGAACGTGCAGAAAGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCGATCATCGCGATTCTCGCCGCCATCGC
CATCCCGGCCTATCAGGACTATCTGATCCGCACGCAGGTGTCGGAAGGTGCGGTGCTGACTGACGGTGCCAAGACCGCCA
TCAGCGAGTTCTATTCGAACACTGGAAGCTTCCCCAGCAAGAATGCCTCCGCCGGCCTGGCCAGCCCAGCCTCGATCACC
GGCAAGTACGTCGCAAAGGTGGATGTCAGCGCTACGACCGGCCAGATCGTTGCGACGTTCGGCAAGGACGCGAACAAAGC
CATCAAGACCAGCACCTTCGTTCTCTCCGCGATTACCAGCACTGGCTCGATCGCCTGGTCCTGCAAAAAGTCGACCGTCG
ATCCGAAGTACCTGCCGACCTCCTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

44.72

100

0.503
  pilE Neisseria gonorrhoeae MS11

44.099

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

45.806

100

0.496
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.714

100

0.49
  pilA Ralstonia pseudosolanacearum GMI1000

39.766

100

0.476
  pilA2 Legionella pneumophila str. Paris

48.201

97.203

0.469
  pilA2 Legionella pneumophila strain ERS1305867

47.482

97.203

0.462
  comP Acinetobacter baylyi ADP1

42.282

100

0.441
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.869

100

0.427
  pilA Acinetobacter baumannii strain A118

40.278

100

0.406
  pilA Pseudomonas aeruginosa PAK

35.374

100

0.364