Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PVLB_RS01710 Genome accession   NC_022738
Coordinates   378584..379798 (+) Length   404 a.a.
NCBI ID   WP_023378226.1    Uniprot ID   -
Organism   Pseudomonas sp. VLB120     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 373584..384798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVLB_RS01695 (PVLB_01655) pilM 376179..377069 (+) 891 WP_023378223.1 type IV pilus biogenesis protein PilM -
  PVLB_RS01700 (PVLB_01660) - 377057..377593 (+) 537 WP_023378224.1 PilN domain-containing protein -
  PVLB_RS01705 (PVLB_01665) - 377650..378597 (+) 948 WP_238546532.1 pilus assembly protein PilP -
  PVLB_RS01710 (PVLB_01670) pilQ 378584..379798 (+) 1215 WP_023378226.1 type IV pilus secretin PilQ Machinery gene
  PVLB_RS01715 (PVLB_01675) aroK 379803..380321 (+) 519 WP_023378227.1 shikimate kinase AroK -
  PVLB_RS01720 (PVLB_01680) aroB 380419..381516 (+) 1098 WP_023378228.1 3-dehydroquinate synthase -
  PVLB_RS01725 (PVLB_01685) - 381532..383157 (+) 1626 WP_023378229.1 AAA family ATPase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43842.09 Da        Isoelectric Point: 4.8595

>NTDB_id=63365 PVLB_RS01710 WP_023378226.1 378584..379798(+) (pilQ) [Pseudomonas sp. VLB120]
MAAPDRGESLSLNFQDVPVRSVLQVMADYAGINLVASDAVQGNITLRLQDVPWDQALELVLRSKGLGRRQEGNVLLVAPA
AELAEQSRGERIGQELEAQLQPLSRALLPIHHAKASELVELLQATLADDGILTGRGSLAVDARTNTLIAHQPADRLAELR
RLVAELDVPLRQVAIEARIVEANVDYEKSLGVRWGGPLYGETAQLGRDLFVDLGVDRATSGVGLGLLRAEVLLDLELSAM
EKSGNGEIISQPKVVTGDKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDGKVIMAVRVTKDEPDYVNA
LNDVPPIRKNEVNARVRVADGETIVIGGVYSTTQNKVIDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAI
AVSR

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=63365 PVLB_RS01710 WP_023378226.1 378584..379798(+) (pilQ) [Pseudomonas sp. VLB120]
ATGGCGGCGCCTGACCGGGGGGAGTCGCTGTCGCTGAATTTCCAGGATGTGCCCGTGCGCTCAGTGTTGCAGGTGATGGC
CGACTACGCGGGCATCAACCTGGTAGCCAGCGATGCCGTGCAAGGCAACATCACGTTGAGATTGCAGGATGTGCCGTGGG
ATCAGGCGCTGGAGCTGGTGTTGCGCAGCAAGGGGCTGGGCCGTCGCCAAGAGGGCAATGTGTTGCTTGTGGCGCCGGCC
GCCGAGTTGGCCGAGCAGTCCCGGGGCGAGCGGATCGGGCAGGAGCTGGAGGCTCAGTTGCAGCCCCTGAGCCGGGCATT
GCTGCCCATCCACCATGCCAAGGCTTCTGAACTTGTCGAGTTGCTCCAGGCGACCCTGGCGGACGACGGTATTCTGACCG
GTCGCGGCAGCCTGGCGGTCGATGCGCGTACCAACACGCTGATCGCCCACCAGCCAGCCGATAGGCTGGCCGAATTGCGC
AGATTGGTGGCCGAACTGGACGTGCCGCTGCGGCAAGTGGCGATCGAGGCGCGCATCGTCGAGGCCAATGTCGACTACGA
AAAGAGCCTGGGCGTGCGCTGGGGTGGACCGCTGTATGGCGAAACAGCGCAGTTGGGTCGTGATTTGTTCGTTGACTTGG
GGGTAGACAGGGCGACCTCCGGGGTGGGGTTGGGCTTGCTGCGCGCGGAAGTGCTGCTGGATCTTGAGCTCAGTGCCATG
GAGAAAAGCGGCAACGGCGAAATCATCTCGCAGCCCAAGGTGGTGACGGGCGACAAGGAAACCGCCAGAATTCTCAAAGG
CACCGAAGTGCCATATCAGGAGACCAGCAAGAGCGGCGCCACGTCTGTCGCTTTCCGCGAGGCATCGTTGTCCCTCGAGG
TCACGCCGCAGATCACCCCAGACGGCAAGGTGATCATGGCAGTCAGAGTGACCAAGGACGAGCCGGACTACGTCAACGCC
TTGAACGACGTGCCTCCGATCCGCAAGAACGAGGTCAATGCCAGGGTTCGTGTAGCTGATGGCGAAACGATAGTGATCGG
CGGAGTCTATTCGACCACGCAAAACAAAGTGATCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGT
TTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATT
GCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(239-395)

Bacterial type II/III secretion system short domain (Secretin_N)

(107-172)

Secretin and TonB N terminus short domain (STN)

(33-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.677

100

0.641
  pilQ Acinetobacter baumannii strain A118

40.351

100

0.455
  pilQ Acinetobacter baumannii D1279779

40.351

100

0.455
  comQ Acinetobacter baylyi ADP1

41.163

100

0.455
  pilQ Legionella pneumophila strain ERS1305867

42.788

100

0.441
  pilQ Legionella pneumophila strain Lp02

42.788

100

0.441
  pilQ Vibrio campbellii strain DS40M4

40.185

100

0.431
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.205

100

0.423
  pilQ Vibrio cholerae strain A1552

41.205

100

0.423
  pilQ Neisseria gonorrhoeae strain FA1090

35.817

100

0.369
  pilQ Neisseria meningitidis MC58

35.096

100

0.361
  pilQ Neisseria meningitidis M1080

35.096

100

0.361

Multiple sequence alignment