Detailed information NTG0627979

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY66_RS10535
• Genome accession: NZ_CP087200
• Coordinates: 2352095..2353204 (+)
• Length: 369 a.a.
• NCBI ID: WP_135844731.1
• Uniprot ID: -
• Organism: Pseudomonas viciae strain B21-062
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2347095..2358204

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY66_RS10520 (LOY66_10510)	-	2348101..2349018 (+)	918	WP_256452555.1	sugar kinase	-
LOY66_RS10525 (LOY66_10515)	-	2349100..2349375 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
LOY66_RS10530 (LOY66_10520)	-	2349424..2351961 (-)	2538	WP_135844730.1	PAS domain-containing protein	-
LOY66_RS10535 (LOY66_10525)	pilU	2352095..2353204 (+)	1110	WP_135844731.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY66_RS10540 (LOY66_10530)	-	2353530..2354879 (-)	1350	WP_256452556.1	FAD-dependent oxidoreductase	-
LOY66_RS10545 (LOY66_10535)	-	2355342..2357297 (-)	1956	WP_265072896.1	acetoacetate--CoA ligase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41143.14 Da
• Isoelectric Point: 6.2905

>NTDB_id=627979 LOY66_RS10535 WP_135844731.1 2352095..2353204(+) (pilU) [Pseudomonas viciae strain B21-062]
MEIDPLLRILASQDGSDLYMSTGAPPCVRFEGVLKPLGNQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISMAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPLVLLDTIMQKQGLILFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRSNQFSELKGIMEKSVEQGMQTFDHAL
YALVVEGVIEEEEALKHADSVNNLRLQLKLHAEASPQPQAPSGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=627979 LOY66_RS10535 WP_135844731.1 2352095..2353204(+) (pilU) [Pseudomonas viciae strain B21-062]
ATGGAAATCGATCCCTTGTTGCGAATCCTGGCAAGCCAGGATGGCTCCGACCTCTACATGTCCACCGGGGCGCCACCGTG
TGTGCGGTTCGAGGGCGTGCTCAAGCCCTTGGGCAACCAGGCATTCAAGGTCGGCGACATTGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTCGAGTTCGATCGGGAGCTGGAAATGAACCTGGCGATCTCCATGGCCGGTGTCGGGCGG
TTCCGGGTCAATATATTCAAGCAACGCAACGATGTGTCCATGGTGATACGCAACGTCAAGCTGGACATTCCGCGTTTCGA
AGACTTGAAACTGCCGCTGGTGTTACTGGACACCATTATGCAGAAGCAAGGGCTGATACTGTTCGTTGGTGCGACAGGGT
CGGGCAAGTCGACCTCCCTGGCGGCCCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCATAAGAAATCGATCATCAACCAGCGCGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGTGATCGTGAAACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATTTCCACGCTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTCCCTGAGGAGCGCCGGCCGCAACTGCTGAACGATCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
CCTGGTGCGCACCCGCACCGGTCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCA
GGAGCAATCAGTTTTCTGAACTCAAGGGGATTATGGAAAAGTCCGTGGAGCAAGGGATGCAAACGTTCGACCACGCGCTT
TACGCGCTGGTCGTCGAGGGCGTGATTGAAGAGGAGGAAGCACTGAAGCATGCGGACTCGGTGAACAACCTGCGGCTGCA
GTTGAAATTGCACGCCGAAGCATCACCGCAGCCCCAAGCGCCGTCGGGCGAATGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	96.206	0.539
pilU	Acinetobacter baylyi ADP1	51.821	96.748	0.501
pilU	Vibrio cholerae strain A1552	51.714	94.851	0.491
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90.244	0.363
pilT	Vibrio cholerae strain A1552	40.24	90.244	0.363
pilT	Pseudomonas stutzeri DSM 10701	38.953	93.225	0.363
pilT	Pseudomonas aeruginosa PAK	38.663	93.225	0.36
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36
pilT	Acinetobacter baumannii strain A118	39.82	90.515	0.36
pilT	Acinetobacter baumannii D1279779	39.82	90.515	0.36