Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY51_RS25340 Genome accession   NZ_CP087185
Coordinates   5645227..5646495 (-) Length   422 a.a.
NCBI ID   WP_258646720.1    Uniprot ID   -
Organism   Pseudomonas sichuanensis strain B21-027     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5640227..5651495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY51_RS25325 (LOY51_25285) - 5641888..5643492 (-) 1605 WP_125465908.1 AAA family ATPase -
  LOY51_RS25330 (LOY51_25290) aroB 5643508..5644605 (-) 1098 WP_125465909.1 3-dehydroquinate synthase -
  LOY51_RS25335 (LOY51_25295) aroK 5644704..5645222 (-) 519 WP_110996384.1 shikimate kinase AroK -
  LOY51_RS25340 (LOY51_25300) pilQ 5645227..5646495 (-) 1269 WP_258646720.1 type IV pilus secretin PilQ Machinery gene
  LOY51_RS25345 (LOY51_25305) - 5646425..5647435 (-) 1011 WP_258643560.1 pilus assembly protein PilP -
  LOY51_RS25350 (LOY51_25310) - 5647432..5647968 (-) 537 WP_258643562.1 PilN domain-containing protein -
  LOY51_RS25355 (LOY51_25315) pilM 5647968..5648858 (-) 891 WP_258643565.1 type IV pilus biogenesis protein PilM -
  LOY51_RS25360 (LOY51_25320) - 5649027..5651477 (+) 2451 WP_374046964.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45995.84 Da        Isoelectric Point: 4.9405

>NTDB_id=627588 LOY51_RS25340 WP_258646720.1 5645227..5646495(-) (pilQ) [Pseudomonas sichuanensis strain B21-027]
MKRWSSGVLMLLLGLRATLATAFEKEPISLNFQDVEVRAVLQVMADYVGINLVASDTVQGRISLRLDDVPWQQALDLVLR
SKGLGRRQEGNVLLVAPVAELAEQSRDAHLEQALEAQLEPLQRELIPVHHARAAELAELLLASLADDGILAGRGSLSVDA
RTNTLVVAQPPERIAELRKLVAQLDVPVRQVMIEARIVEAGVDYEKALGVRWGGPLYGQNSRLGSELFVDLGVERGTSGL
GVGLLRSDILLDLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETTKSGATSISFREASLSLEVTPQITPDG
QVVMEVRVTKDEPDFVNALNNVPPIRKNEVNAKVRVADGQTIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=627588 LOY51_RS25340 WP_258646720.1 5645227..5646495(-) (pilQ) [Pseudomonas sichuanensis strain B21-027]
ATGAAAAGGTGGTCGAGCGGGGTTCTGATGCTCCTGCTGGGCTTGAGGGCGACGCTGGCGACGGCGTTTGAGAAAGAGCC
CATATCGCTGAACTTCCAGGACGTGGAAGTGCGCGCGGTGCTGCAGGTCATGGCTGACTACGTGGGGATCAACCTGGTGG
CCAGCGATACGGTCCAGGGCCGCATCAGTCTGCGCCTTGACGATGTGCCGTGGCAGCAGGCGCTGGATCTGGTGCTGCGC
AGCAAAGGGCTGGGGCGGCGCCAGGAGGGTAATGTGCTGCTGGTCGCGCCGGTTGCCGAGCTTGCCGAGCAGTCGCGCGA
TGCCCACCTGGAGCAGGCGCTGGAGGCGCAACTGGAACCCTTGCAGCGCGAGCTGATTCCGGTCCATCACGCCCGGGCTG
CGGAACTGGCCGAGTTGCTGCTGGCCAGCCTTGCCGATGACGGCATCCTGGCCGGGCGCGGCAGCCTCAGCGTGGATGCA
CGGACCAATACCCTGGTGGTGGCCCAGCCGCCAGAGCGGATCGCCGAGCTGCGCAAGCTGGTGGCCCAGCTCGATGTGCC
CGTGCGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGGCGTCGATTACGAAAAGGCACTGGGTGTGCGTTGGGGAG
GCCCTTTGTACGGGCAGAACAGCCGGTTGGGCAGCGAGCTGTTCGTCGACCTGGGGGTGGAGCGGGGTACGTCGGGGCTG
GGTGTGGGCCTGCTGCGCAGTGACATCCTGCTGGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGTAACGGCGAGATCAT
CTCGCAGCCCAAGGTGGTCACCGCCGACAAAGAGACCGCGCGCATCATCAAGGGCACCGAGGTGCCCTACCAGGAGACGA
CCAAGAGTGGTGCCACCTCGATCTCGTTCCGCGAGGCCTCGCTGTCGCTGGAAGTAACGCCGCAGATCACCCCGGATGGC
CAGGTGGTGATGGAGGTGCGGGTGACCAAGGATGAGCCGGATTTCGTCAATGCCCTGAACAATGTGCCGCCGATCCGCAA
GAATGAGGTCAATGCCAAGGTGCGGGTGGCCGATGGCCAGACCATTGTGATTGGCGGTGTCTACTCGACGTCGCAGAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.895

100

0.6
  comQ Acinetobacter baylyi ADP1

44.989

100

0.479
  pilQ Acinetobacter baumannii D1279779

41.703

100

0.453
  pilQ Acinetobacter baumannii strain A118

41.703

100

0.453
  pilQ Vibrio campbellii strain DS40M4

42.453

100

0.427
  pilQ Legionella pneumophila strain Lp02

43.062

99.052

0.427
  pilQ Legionella pneumophila strain ERS1305867

43.062

99.052

0.427
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.755

99.763

0.427
  pilQ Vibrio cholerae strain A1552

42.755

99.763

0.427
  pilQ Neisseria gonorrhoeae strain FA1090

37.619

99.526

0.374
  pilQ Neisseria meningitidis MC58

36.667

99.526

0.365
  pilQ Neisseria meningitidis M1080

36.429

99.526

0.363