Detailed information NTG0627549

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY35_RS10650
• Genome accession: NZ_CP087184
• Coordinates: 2340402..2341517 (+)
• Length: 371 a.a.
• NCBI ID: WP_258632326.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-028
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2335402..2346517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY35_RS10635 (LOY35_10620)	-	2336374..2337291 (+)	918	WP_258632323.1	sugar kinase	-
LOY35_RS10640 (LOY35_10625)	-	2337422..2337697 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
LOY35_RS10645 (LOY35_10630)	-	2337741..2340278 (-)	2538	WP_258632325.1	PAS domain-containing protein	-
LOY35_RS10650 (LOY35_10635)	pilU	2340402..2341517 (+)	1116	WP_258632326.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY35_RS10655 (LOY35_10640)	-	2341937..2343892 (-)	1956	WP_258632328.1	acetoacetate--CoA ligase	-
LOY35_RS10660 (LOY35_10645)	hbdH	2344102..2344878 (-)	777	WP_258632330.1	3-hydroxybutyrate dehydrogenase	-
LOY35_RS10665 (LOY35_10650)	-	2344886..2346277 (-)	1392	WP_258632331.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41094.00 Da
• Isoelectric Point: 5.8696

>NTDB_id=627549 LOY35_RS10650 WP_258632326.1 2340402..2341517(+) (pilU) [Pseudomonas sp. B21-028]
MEIDPLLRILASQEGSDLYLSTGAPPCAKFEGVLKPLGSQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLSGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPKVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRSQLLSDLGNNLQAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGVLDEEEALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=627549 LOY35_RS10650 WP_258632326.1 2340402..2341517(+) (pilU) [Pseudomonas sp. B21-028]
ATGGAAATCGATCCCTTGTTACGCATCCTGGCCAGCCAGGAAGGCTCCGATCTTTACCTGTCCACCGGGGCACCGCCGTG
TGCCAAGTTCGAAGGCGTGCTCAAGCCCTTGGGCAGCCAGGCGTTCAAGGTCGGTGACATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAACGCCTTGAGTTCGATCGGGAATTGGAAATGAACCTGGCCATTTCGTTGTCGGGTGTCGGGCGA
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
GGACCTGAAACTGCCCAAGGTACTGCTGGACACCATCATGCAGAAGCAGGGGTTGATGCTGTTCGTCGGCGCGACGGGAT
CAGGCAAGTCCACTTCCCTGGCGGCGCTGATCGATTATCGCAATCGCAACAGCAGCGGCCATATCATCACCATCGAGGAC
CCGGTGGAGTACATCCACCGGCACAAGAAGTCGATCATCAACCAGCGGGAGGTCGGTGTCGACACCCGCAGTTTCCATGC
CGCCCTCAAGAACACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAAATTCGCGACCGGGAAACCATGGAACACG
CCCTGGCCTTTGCCGACACCGGGCACCTGGTGATCTCTACCCTGCATGCTCACAATGCCAATCAGGCCCTGGACCGGGTG
ATCAACTTCTTCCCCGAGGAACGCCGGTCGCAACTGTTGAGCGACCTGGGCAACAACCTCCAGGCATTCGTGTCCCAGCG
CCTGGTGCGCACCCGAACCGGCCAGCGCCGGGCCGCGGTGGAGGTCATGCTCGGCTCGCCCACCGTGGCCGACCTGATCC
GGCGCAACGAACTGGGCGAACTCAAGGGCATCATGGAGAAGTCGGAAGAACTCGGCATGCAGACCTTTGACCAAGCCCTG
TTCAACCTGGTGGTGGAAGGGGTGCTCGACGAGGAAGAAGCCCTGAAGAACGCCGACTCGGCCAACAACCTGCGCTTGCG
GTTGAAGCTGCATGCGGAGTCGGGCGCCGCGCCCCCGGCCGATCCGGCGGCGGGGGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	51.515	97.844	0.504
pilU	Vibrio cholerae strain A1552	52	94.34	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.692	98.922	0.383
pilT	Pseudomonas stutzeri DSM 10701	39.826	92.722	0.369
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364
pilT	Acinetobacter baumannii strain A118	40.12	90.027	0.361
pilT	Acinetobacter baumannii D1279779	40.12	90.027	0.361
pilT	Acinetobacter nosocomialis M2	40.12	90.027	0.361