Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY46_RS25370 Genome accession   NZ_CP087165
Coordinates   5493795..5495063 (-) Length   422 a.a.
NCBI ID   WP_258706654.1    Uniprot ID   -
Organism   Pseudomonas sichuanensis strain B21-052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5488795..5500063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY46_RS25355 (LOY46_25325) - 5490456..5492060 (-) 1605 WP_258705284.1 AAA family ATPase -
  LOY46_RS25360 (LOY46_25330) aroB 5492076..5493173 (-) 1098 WP_258705285.1 3-dehydroquinate synthase -
  LOY46_RS25365 (LOY46_25335) aroK 5493272..5493790 (-) 519 WP_110996384.1 shikimate kinase AroK -
  LOY46_RS25370 (LOY46_25340) pilQ 5493795..5495063 (-) 1269 WP_258706654.1 type IV pilus secretin PilQ Machinery gene
  LOY46_RS25375 (LOY46_25345) - 5494993..5496003 (-) 1011 WP_258705286.1 pilus assembly protein PilP -
  LOY46_RS25380 (LOY46_25350) - 5496000..5496518 (-) 519 WP_258706655.1 PilN domain-containing protein -
  LOY46_RS25385 (LOY46_25355) pilM 5496536..5497426 (-) 891 WP_258705287.1 type IV pilus biogenesis protein PilM -
  LOY46_RS25390 (LOY46_25360) - 5497595..5500045 (+) 2451 WP_374045905.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45984.86 Da        Isoelectric Point: 4.9405

>NTDB_id=627077 LOY46_RS25370 WP_258706654.1 5493795..5495063(-) (pilQ) [Pseudomonas sichuanensis strain B21-052]
MKRWPSGVLMLLLGLRATLATAFEKEPISLNFQDVEVRAVLQVMADYVGINLVASDTVQGRISLRLDDVPWQQALDLVLR
SKGLGRRQEGNVLLVAPVAELAEQSRDAHLEQALEAQLEPLQRELIPVHHARAAELAELLLASLADDGILAGRGSLSVDA
RTNTLVVAQPPERIAELRKLVAQLDVPVRQVMIEARIVEAGVDYEKVLGVRWGGPLNGQNSRLGSELFVDLGVERGTSGL
GVGLLRSDILLDLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETTKSGATSISFREASLSLEVTPQITPDG
QVVMEVRVTKDEPDFVNALNNVPPIRKNEVNAKVRVADGQTIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=627077 LOY46_RS25370 WP_258706654.1 5493795..5495063(-) (pilQ) [Pseudomonas sichuanensis strain B21-052]
ATGAAAAGGTGGCCGAGCGGGGTTCTGATGCTCCTGCTGGGCTTGAGGGCGACGCTGGCGACGGCGTTTGAGAAAGAGCC
CATATCGCTGAACTTCCAGGACGTGGAAGTGCGCGCGGTGCTGCAGGTCATGGCTGACTACGTGGGGATCAACCTGGTGG
CCAGCGATACGGTCCAGGGCCGCATCAGCCTGCGCCTTGACGATGTGCCGTGGCAGCAGGCGCTGGATCTGGTGCTGCGC
AGCAAGGGGCTGGGGCGGCGCCAGGAGGGTAATGTGCTGCTGGTCGCGCCGGTTGCCGAACTTGCCGAGCAGTCGCGCGA
TGCCCACCTGGAGCAGGCGCTGGAGGCGCAACTGGAACCCTTGCAGCGCGAGCTGATTCCGGTCCATCACGCCCGGGCTG
CGGAACTGGCCGAGTTGCTGCTGGCCAGCCTTGCCGATGACGGCATCCTGGCCGGGCGCGGCAGCCTCAGCGTGGATGCA
CGGACCAATACCCTGGTGGTGGCCCAGCCGCCAGAGCGGATCGCCGAGCTGCGCAAGCTGGTGGCGCAGCTCGATGTGCC
CGTACGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGGCGTCGATTACGAAAAGGTACTGGGCGTGCGTTGGGGAG
GCCCTTTGAATGGGCAGAACAGCCGGTTGGGCAGCGAGCTGTTCGTCGACCTGGGGGTGGAGCGGGGCACGTCGGGGCTG
GGTGTGGGCCTGCTGCGCAGCGACATACTGCTGGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGCAACGGCGAGATCAT
CTCGCAGCCCAAGGTGGTCACTGCCGACAAAGAGACCGCGCGCATCATCAAGGGTACCGAGGTGCCTTACCAGGAAACGA
CCAAGAGTGGTGCCACCTCGATCTCGTTCCGCGAGGCCTCGCTGTCGCTGGAAGTAACGCCGCAGATCACCCCGGATGGC
CAGGTGGTGATGGAGGTGCGGGTGACCAAGGATGAGCCGGATTTCGTCAACGCCCTGAACAATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTGCGGGTGGCCGATGGCCAGACCATTGTGATTGGCGGCGTCTACTCGACGTCGCAGAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.666

100

0.597
  comQ Acinetobacter baylyi ADP1

44.989

100

0.479
  pilQ Acinetobacter baumannii strain A118

41.266

100

0.448
  pilQ Acinetobacter baumannii D1279779

41.266

100

0.448
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.993

99.763

0.429
  pilQ Vibrio cholerae strain A1552

42.993

99.763

0.429
  pilQ Vibrio campbellii strain DS40M4

42.453

100

0.427
  pilQ Legionella pneumophila strain Lp02

43.062

99.052

0.427
  pilQ Legionella pneumophila strain ERS1305867

43.062

99.052

0.427
  pilQ Neisseria gonorrhoeae strain FA1090

37.381

99.526

0.372
  pilQ Neisseria meningitidis MC58

36.905

99.526

0.367
  pilQ Neisseria meningitidis M1080

36.667

99.526

0.365