Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   MRX62_RS07895 Genome accession   NZ_CP104027
Coordinates   1646636..1647103 (-) Length   155 a.a.
NCBI ID   WP_023907060.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain 50088     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1641636..1652103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRX62_RS07885 (MRX62_07875) - 1642556..1643968 (+) 1413 WP_060871719.1 sigma-54 dependent transcriptional regulator -
  MRX62_RS07890 (MRX62_07880) pilB 1644062..1645795 (-) 1734 WP_060871718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRX62_RS07895 (MRX62_07885) pilA/pilAI 1646636..1647103 (-) 468 WP_023907060.1 pilin Machinery gene
  MRX62_RS07900 (MRX62_07890) pilA 1648401..1648847 (-) 447 WP_060871716.1 pilin Machinery gene
  MRX62_RS07905 (MRX62_07895) - 1649178..1649357 (-) 180 WP_195780022.1 hypothetical protein -
  MRX62_RS07910 (MRX62_07900) pilC 1649287..1650489 (+) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  MRX62_RS07915 (MRX62_07905) pilD 1650496..1651359 (+) 864 WP_010894958.1 A24 family peptidase Machinery gene
  MRX62_RS07920 (MRX62_07910) coaE 1651371..1651994 (+) 624 WP_023907058.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15916.15 Da        Isoelectric Point: 8.5063

>NTDB_id=626443 MRX62_RS07895 WP_023907060.1 1646636..1647103(-) (pilA/pilAI) [Xylella fastidiosa subsp. pauca strain 50088]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEITPGKVQAEILFSDAGTKTAITTPETIGLRTATTR
CSSIAVNLTPSAGTGTIVCTITGNSQVNGQTITWTRSADNTSGQGGTNNGGLWSCTTTVATTLSPSTCTSTAKNG

Nucleotide


Download         Length: 468 bp        

>NTDB_id=626443 MRX62_RS07895 WP_023907060.1 1646636..1647103(-) (pilA/pilAI) [Xylella fastidiosa subsp. pauca strain 50088]
ATGAAAAAGCAACAAGGTTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTCGCAGCTATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATTACACCCGGCAAAGTACAAGCCG
AGATTCTTTTTAGTGATGCAGGAACAAAAACAGCCATCACTACACCGGAAACTATAGGTTTGCGTACCGCTACTACTCGT
TGCAGTTCCATTGCTGTCAATCTCACACCGAGCGCTGGCACTGGCACCATCGTATGTACGATTACTGGCAATTCTCAGGT
CAACGGTCAAACCATTACGTGGACTCGTAGTGCCGATAACACCTCAGGTCAAGGTGGCACCAATAATGGTGGTTTGTGGA
GCTGCACCACAACCGTTGCAACAACGTTGAGCCCATCAACGTGCACTTCTACTGCTAAGAATGGTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.295

96.129

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.857

94.839

0.406
  pilA Vibrio cholerae C6706

40.26

99.355

0.4
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.26

99.355

0.4
  pilA Vibrio cholerae strain A1552

40.26

99.355

0.4
  pilA Pseudomonas aeruginosa PAK

38.75

100

0.4
  pilA Acinetobacter baumannii strain A118

39.474

98.065

0.387
  comP Acinetobacter baylyi ADP1

37.179

100

0.374
  pilA Vibrio parahaemolyticus RIMD 2210633

41.606

88.387

0.368