Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MRX62_RS07900 Genome accession   NZ_CP104027
Coordinates   1648401..1648847 (-) Length   148 a.a.
NCBI ID   WP_060871716.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain 50088     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643401..1653847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRX62_RS07890 (MRX62_07880) pilB 1644062..1645795 (-) 1734 WP_060871718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRX62_RS07895 (MRX62_07885) pilA/pilAI 1646636..1647103 (-) 468 WP_023907060.1 pilin Machinery gene
  MRX62_RS07900 (MRX62_07890) pilA 1648401..1648847 (-) 447 WP_060871716.1 pilin Machinery gene
  MRX62_RS07905 (MRX62_07895) - 1649178..1649357 (-) 180 WP_195780022.1 hypothetical protein -
  MRX62_RS07910 (MRX62_07900) pilC 1649287..1650489 (+) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  MRX62_RS07915 (MRX62_07905) pilD 1650496..1651359 (+) 864 WP_010894958.1 A24 family peptidase Machinery gene
  MRX62_RS07920 (MRX62_07910) coaE 1651371..1651994 (+) 624 WP_023907058.1 dephospho-CoA kinase -
  MRX62_RS07925 (MRX62_07915) - 1652261..1653598 (-) 1338 WP_060871715.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15376.49 Da        Isoelectric Point: 8.4988

>NTDB_id=626444 MRX62_RS07900 WP_060871716.1 1648401..1648847(-) (pilA) [Xylella fastidiosa subsp. pauca strain 50088]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKSTSNASDIGLRTDTTRCGI
TVKVDAAGTANITCKVKGNSQVNDKTIAWDRTSDNSAGTNGVNNGGVWTCSSNVTSDALRPSGCIASK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=626444 MRX62_RS07900 WP_060871716.1 1648401..1648847(-) (pilA) [Xylella fastidiosa subsp. pauca strain 50088]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTCCTAGCAGCCATCGCGCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACCGCGGCACTGGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGAGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGGATT
ACCGTTAAAGTCGATGCCGCTGGCACTGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGCTTGGGATCGTACCTCAGATAACTCTGCAGGCACCAATGGTGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCAACG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCTCTAAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA Vibrio cholerae strain A1552

38.065

100

0.399
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.065

100

0.399
  pilA Vibrio cholerae C6706

38.065

100

0.399
  pilA Acinetobacter baumannii strain A118

39.333

100

0.399