Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MRX62_RS07915 Genome accession   NZ_CP104027
Coordinates   1650496..1651359 (+) Length   287 a.a.
NCBI ID   WP_010894958.1    Uniprot ID   Q9PAI1
Organism   Xylella fastidiosa subsp. pauca strain 50088     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1645496..1656359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRX62_RS07895 (MRX62_07885) pilA/pilAI 1646636..1647103 (-) 468 WP_023907060.1 pilin Machinery gene
  MRX62_RS07900 (MRX62_07890) pilA 1648401..1648847 (-) 447 WP_060871716.1 pilin Machinery gene
  MRX62_RS07905 (MRX62_07895) - 1649178..1649357 (-) 180 WP_195780022.1 hypothetical protein -
  MRX62_RS07910 (MRX62_07900) pilC 1649287..1650489 (+) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  MRX62_RS07915 (MRX62_07905) pilD 1650496..1651359 (+) 864 WP_010894958.1 A24 family peptidase Machinery gene
  MRX62_RS07920 (MRX62_07910) coaE 1651371..1651994 (+) 624 WP_023907058.1 dephospho-CoA kinase -
  MRX62_RS07925 (MRX62_07915) - 1652261..1653598 (-) 1338 WP_060871715.1 HAMP domain-containing sensor histidine kinase -
  MRX62_RS07930 (MRX62_07920) - 1653591..1654268 (-) 678 WP_010894955.1 response regulator transcription factor -
  MRX62_RS07935 (MRX62_07925) rimK 1654954..1655818 (-) 865 Protein_1544 30S ribosomal protein S6--L-glutamate ligase -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31776.90 Da        Isoelectric Point: 8.6709

>NTDB_id=626446 MRX62_RS07915 WP_010894958.1 1650496..1651359(+) (pilD) [Xylella fastidiosa subsp. pauca strain 50088]
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSTDNLFMAAKPALLGAVVGYLSLWIVAALFKLIFNKEGMGHGDFKMLAALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Nucleotide


Download         Length: 864 bp        

>NTDB_id=626446 MRX62_RS07915 WP_010894958.1 1650496..1651359(+) (pilD) [Xylella fastidiosa subsp. pauca strain 50088]
ATGGCATTTCTTGATCAGCACCCCGGACTCGGCTATCCCCTTGCGTCCGGTTTGGGGTTGTTGCTTGGTAGTTTTCTCAA
TGTGGTGATCCTGCGTTTGCCGCGCCGTTTGGAATGGCAATGGAAGCGCGATGCGCGCGAAATCCTGGAGATGCCGGACA
TTTATGATCCGCCACCACCGGGAATTGTGGTCGAGCCTTCCCACGATCCAGTGACTGGTGATCGGTTGAAGTGGTGGGAA
AACATTCCGGTGCTTAGCTGGTTGTTGCTACGTGGACGTTCGCGTTACAGCGGCAAGTCAATCTCGTTGCAGTATCCGTT
GGTCGAATTGCTGACTTGCCTGCTGGTGCTGGCCAGTGTTTGGCGTTTTGGATTCGGTTGGCATGGTTTTGGGGCAATTG
TGTTCAGTTGTTTCCTGGTTGCGTTGGCTGGGATTGATTTGCGTACTCGGCTCTTACCTGACCAGTTAACTTTAATGCTC
ATGTGGCTGGGTTTGATTGGATCGACGGATAACTTGTTTATGGCAGCCAAACCTGCGTTATTGGGTGCTGTAGTTGGTTA
TTTATCTCTGTGGATTGTGGCGGCGTTGTTCAAACTGATCTTTAACAAGGAAGGGATGGGCCATGGTGACTTCAAAATGC
TGGCCGCGCTTGGGGCATGGTGTGGGTTGAAGGGTATTCTCCCGATCATTTTGATTTCATCACTGGCTGGAGTGGTATTG
GGCTCCGTTTGGCTTGTTGTGCGTGGTCATAACCGTACAACGATGATTCCGTTTGGACCTTATCTGGCAGTTGGTGGATG
GATCGTGTTCTTTTTCGGCGATCAGATCATCCATGCTTATCTGGAGCTATTAAGTATCGGGTGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9PAI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

42.403

98.606

0.418
  pilD Vibrio campbellii strain DS40M4

41.754

99.303

0.415
  pilD Acinetobacter nosocomialis M2

42.049

98.606

0.415
  pilD Acinetobacter baumannii D1279779

42.049

98.606

0.415
  pilD Neisseria gonorrhoeae MS11

44.03

93.38

0.411