Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LMK00_RS00650 Genome accession   NZ_CP086395
Coordinates   134419..135363 (+) Length   314 a.a.
NCBI ID   WP_252175525.1    Uniprot ID   A0A9Q8Y1W2
Organism   Lactococcus formosensis strain I4/6O     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 129419..140363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMK00_RS00635 (LMK00_00635) - 130671..132275 (-) 1605 WP_252175524.1 GH25 family lysozyme -
  LMK00_RS00640 (LMK00_00640) guaB 132449..133930 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  LMK00_RS00645 (LMK00_00645) rpsU 134055..134231 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  LMK00_RS00650 (LMK00_00650) comGA 134419..135363 (+) 945 WP_252175525.1 competence type IV pilus ATPase ComGA Machinery gene
  LMK00_RS00655 (LMK00_00655) comGB 135302..136321 (+) 1020 WP_275692263.1 competence type IV pilus assembly protein ComGB Machinery gene
  LMK00_RS00660 (LMK00_00660) comYC 136340..136645 (+) 306 WP_252175526.1 competence type IV pilus major pilin ComGC Machinery gene
  LMK00_RS12085 comGD 136620..137036 (+) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  LMK00_RS12090 comGE 137068..137301 (+) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  LMK00_RS00670 (LMK00_00670) comGF 137282..137722 (+) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  LMK00_RS00675 (LMK00_00675) - 137958..138803 (+) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  LMK00_RS00680 (LMK00_00680) - 138890..139759 (-) 870 WP_252175527.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35775.09 Da        Isoelectric Point: 6.7701

>NTDB_id=624720 LMK00_RS00650 WP_252175525.1 134419..135363(+) (comGA) [Lactococcus formosensis strain I4/6O]
MIQVLAKTLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRFRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVEAEFVQLQVNKVIGNDYDTLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAIAAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=624720 LMK00_RS00650 WP_252175525.1 134419..135363(+) (comGA) [Lactococcus formosensis strain I4/6O]
ATGATACAGGTTTTAGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCACATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTCCGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGAGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTGTACAACTTCAAGTCAATAAAGTTATAGGAAATGATTACGA
TACCTTGATTAAGCTTTCGCTTCGCCACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTACTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATCGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.016

100

0.532
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.077

99.682

0.519
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.923

99.363

0.516
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.757

99.682

0.516
  comYA Streptococcus mutans UA159

50.316

100

0.506
  comYA Streptococcus mutans UA140

50.316

100

0.506