Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LMK00_RS00655 Genome accession   NZ_CP086395
Coordinates   135302..136321 (+) Length   339 a.a.
NCBI ID   WP_275692263.1    Uniprot ID   -
Organism   Lactococcus formosensis strain I4/6O     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 130302..141321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMK00_RS00635 (LMK00_00635) - 130671..132275 (-) 1605 WP_252175524.1 GH25 family lysozyme -
  LMK00_RS00640 (LMK00_00640) guaB 132449..133930 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  LMK00_RS00645 (LMK00_00645) rpsU 134055..134231 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  LMK00_RS00650 (LMK00_00650) comGA 134419..135363 (+) 945 WP_252175525.1 competence type IV pilus ATPase ComGA Machinery gene
  LMK00_RS00655 (LMK00_00655) comGB 135302..136321 (+) 1020 WP_275692263.1 competence type IV pilus assembly protein ComGB Machinery gene
  LMK00_RS00660 (LMK00_00660) comYC 136340..136645 (+) 306 WP_252175526.1 competence type IV pilus major pilin ComGC Machinery gene
  LMK00_RS12085 comGD 136620..137036 (+) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  LMK00_RS12090 comGE 137068..137301 (+) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  LMK00_RS00670 (LMK00_00670) comGF 137282..137722 (+) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  LMK00_RS00675 (LMK00_00675) - 137958..138803 (+) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  LMK00_RS00680 (LMK00_00680) - 138890..139759 (-) 870 WP_252175527.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39363.97 Da        Isoelectric Point: 9.0696

>NTDB_id=624721 LMK00_RS00655 WP_275692263.1 135302..136321(+) (comGB) [Lactococcus formosensis strain I4/6O]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGVEFLQILDSGQKFEDALRFYPIFTKELALIVEYGELKAKLGQELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=624721 LMK00_RS00655 WP_275692263.1 135302..136321(+) (comGB) [Lactococcus formosensis strain I4/6O]
ATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATCCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGGCTAACAGAAGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTAAATTTTTATGAGGATGTT
GTGACTCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTACACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCATC
TTTTTAGGTTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCCCTTCGATTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGCTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.254

99.705

0.531
  comGB/cglB Streptococcus mitis NCTC 12261

51.642

98.82

0.51
  comYB Streptococcus gordonii str. Challis substr. CH1

51.796

98.525

0.51
  comGB/cglB Streptococcus pneumoniae R6

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

98.82

0.504
  comGB/cglB Streptococcus mitis SK321

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae D39

51.045

98.82

0.504
  comYB Streptococcus mutans UA140

51.429

92.92

0.478
  comYB Streptococcus mutans UA159

51.111

92.92

0.475