Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LL045_RS11420 Genome accession   NZ_CP086083
Coordinates   2318642..2319580 (-) Length   312 a.a.
NCBI ID   WP_394530221.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain EIP20A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2313642..2324580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL045_RS11380 (LL045_11350) - 2314530..2315372 (-) 843 WP_038602352.1 metal ABC transporter substrate-binding protein -
  LL045_RS11385 (LL045_11355) - 2315369..2315806 (-) 438 WP_021721874.1 zinc-dependent MarR family transcriptional regulator -
  LL045_RS11390 (LL045_11360) comGG 2315887..2316162 (-) 276 WP_021721873.1 hypothetical protein Machinery gene
  LL045_RS11395 (LL045_11365) comGF 2316210..2316656 (-) 447 WP_021721872.1 competence type IV pilus minor pilin ComGF Machinery gene
  LL045_RS11400 (LL045_11370) comGE 2316619..2316915 (-) 297 WP_038602356.1 competence type IV pilus minor pilin ComGE Machinery gene
  LL045_RS11405 (LL045_11375) comGD 2316887..2317318 (-) 432 WP_080651959.1 competence type IV pilus minor pilin ComGD Machinery gene
  LL045_RS11410 (LL045_11380) comGC 2317278..2317661 (-) 384 WP_080651958.1 competence type IV pilus major pilin ComGC Machinery gene
  LL045_RS11415 (LL045_11385) comGB 2317675..2318748 (-) 1074 WP_394530628.1 competence type IV pilus assembly protein ComGB Machinery gene
  LL045_RS11420 (LL045_11390) comGA 2318642..2319580 (-) 939 WP_394530221.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35623.89 Da        Isoelectric Point: 6.4008

>NTDB_id=622405 LL045_RS11420 WP_394530221.1 2318642..2319580(-) (comGA) [Lactococcus lactis subsp. lactis strain EIP20A]
MIPKMAKELIQKAIDRGASDIYLITSKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIIGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTSTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=622405 LL045_RS11420 WP_394530221.1 2318642..2319580(-) (comGA) [Lactococcus lactis subsp. lactis strain EIP20A]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAAAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTACTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTACGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTGGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGGTCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCTGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCATTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTATTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGATATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACATCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.244

100

0.772
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.305

99.679

0.551
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.341

99.679

0.542
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.376

99.679

0.532
  comYA Streptococcus mutans UA140

52.564

100

0.526
  comYA Streptococcus mutans UA159

52.564

100

0.526
  comYA Streptococcus gordonii str. Challis substr. CH1

51.757

100

0.519