Detailed information NTG0622400

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL045_RS11395
• Genome accession: NZ_CP086083
• Coordinates: 2316210..2316656 (-)
• Length: 148 a.a.
• NCBI ID: WP_021721872.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP20A
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2311210..2321656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL045_RS11360 (LL045_11330)	-	2311328..2311705 (-)	378	WP_021723060.1	pyridoxamine 5'-phosphate oxidase family protein	-
LL045_RS11365 (LL045_11335)	-	2311909..2312775 (+)	867	WP_394530217.1	RluA family pseudouridine synthase	-
LL045_RS11370 (LL045_11340)	-	2312813..2313622 (-)	810	WP_038602349.1	metal ABC transporter permease	-
LL045_RS11375 (LL045_11345)	-	2313615..2314352 (-)	738	WP_021723055.1	metal ABC transporter ATP-binding protein	-
LL045_RS11380 (LL045_11350)	-	2314530..2315372 (-)	843	WP_038602352.1	metal ABC transporter substrate-binding protein	-
LL045_RS11385 (LL045_11355)	-	2315369..2315806 (-)	438	WP_021721874.1	zinc-dependent MarR family transcriptional regulator	-
LL045_RS11390 (LL045_11360)	comGG	2315887..2316162 (-)	276	WP_021721873.1	hypothetical protein	Machinery gene
LL045_RS11395 (LL045_11365)	comGF	2316210..2316656 (-)	447	WP_021721872.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL045_RS11400 (LL045_11370)	comGE	2316619..2316915 (-)	297	WP_038602356.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL045_RS11405 (LL045_11375)	comGD	2316887..2317318 (-)	432	WP_080651959.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL045_RS11410 (LL045_11380)	comGC	2317278..2317661 (-)	384	WP_080651958.1	competence type IV pilus major pilin ComGC	Machinery gene
LL045_RS11415 (LL045_11385)	comGB	2317675..2318748 (-)	1074	WP_394530628.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL045_RS11420 (LL045_11390)	comGA	2318642..2319580 (-)	939	WP_394530221.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17156.02 Da
• Isoelectric Point: 9.0730

>NTDB_id=622400 LL045_RS11395 WP_021721872.1 2316210..2316656(-) (comGF) [Lactococcus lactis subsp. lactis strain EIP20A]
MERKFCDLNLKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQMAISQSDSIKDWQIFCQQMRFELSGTKLDKVEQNFL
YVTKDKKLRFGFMNDDFRKTDDKGQGYQPMLYDIKGAKIQATKNLITIRIDFNQGGERTFIYQFPEDK

Nucleotide

• Length: 447 bp

>NTDB_id=622400 LL045_RS11395 WP_021721872.1 2316210..2316656(-) (comGF) [Lactococcus lactis subsp. lactis strain EIP20A]
ATGGAAAGGAAATTTTGCGACTTGAACCTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGATCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAATGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAAGGTAGAACAGAATTTTCTA
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAATGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGGGGCTAAGATTCAAGCTACTAAAAATCTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGACAAATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	78.014	95.27	0.743
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	42.143	94.595	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.714	94.595	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	40.714	94.595	0.385