Detailed information NTG0622404

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: LL045_RS11415
• Genome accession: NZ_CP086083
• Coordinates: 2317675..2318748 (-)
• Length: 357 a.a.
• NCBI ID: WP_394530628.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP20A
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2312675..2323748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL045_RS11370 (LL045_11340)	-	2312813..2313622 (-)	810	WP_038602349.1	metal ABC transporter permease	-
LL045_RS11375 (LL045_11345)	-	2313615..2314352 (-)	738	WP_021723055.1	metal ABC transporter ATP-binding protein	-
LL045_RS11380 (LL045_11350)	-	2314530..2315372 (-)	843	WP_038602352.1	metal ABC transporter substrate-binding protein	-
LL045_RS11385 (LL045_11355)	-	2315369..2315806 (-)	438	WP_021721874.1	zinc-dependent MarR family transcriptional regulator	-
LL045_RS11390 (LL045_11360)	comGG	2315887..2316162 (-)	276	WP_021721873.1	hypothetical protein	Machinery gene
LL045_RS11395 (LL045_11365)	comGF	2316210..2316656 (-)	447	WP_021721872.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL045_RS11400 (LL045_11370)	comGE	2316619..2316915 (-)	297	WP_038602356.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL045_RS11405 (LL045_11375)	comGD	2316887..2317318 (-)	432	WP_080651959.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL045_RS11410 (LL045_11380)	comGC	2317278..2317661 (-)	384	WP_080651958.1	competence type IV pilus major pilin ComGC	Machinery gene
LL045_RS11415 (LL045_11385)	comGB	2317675..2318748 (-)	1074	WP_394530628.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL045_RS11420 (LL045_11390)	comGA	2318642..2319580 (-)	939	WP_394530221.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40373.54 Da
• Isoelectric Point: 10.3681

>NTDB_id=622404 LL045_RS11415 WP_394530628.1 2317675..2318748(-) (comGB) [Lactococcus lactis subsp. lactis strain EIP20A]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQSLSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLIHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQRSRIFSEVGKNLDLELNAGQSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWKQFFTKID
RLMQLIQPLVFVFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=622404 LL045_RS11415 WP_394530628.1 2317675..2318748(-) (comGB) [Lactococcus lactis subsp. lactis strain EIP20A]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACATCTACTGAAGCC
AAGAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAATCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGAACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTATTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTGCCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGATTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATTTAATTGCTCAAGGTGTCGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAGAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCAGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGAAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTGTTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCTATGTTACT
CCCCATCTATTCAAATATGGGTTCAGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.698	95.518	0.79
comYB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comYB	Streptococcus mutans UA159	52.786	95.518	0.504
comYB	Streptococcus mutans UA140	52.786	95.518	0.504
comGB/cglB	Streptococcus pneumoniae TIGR4	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae D39	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae R6	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis SK321	50.448	93.838	0.473
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471