Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LJF99_RS00225 Genome accession   NZ_CP085533
Coordinates   39549..40688 (-) Length   379 a.a.
NCBI ID   WP_011898593.1    Uniprot ID   A2T1B1
Organism   Aeromonas salmonicida subsp. salmonicida strain 890054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34549..45688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJF99_RS00200 (LJF99_00200) - 34550..34777 (+) 228 WP_005314583.1 FeoA family protein -
  LJF99_RS00205 (LJF99_00205) feoB 34774..37044 (+) 2271 WP_005314592.1 Fe(2+) transporter permease subunit FeoB -
  LJF99_RS00210 (LJF99_00210) - 37041..37283 (+) 243 WP_005314594.1 FeoC-like transcriptional regulator -
  LJF99_RS00215 (LJF99_00215) - 37379..37858 (-) 480 WP_005314596.1 WbuC family cupin fold metalloprotein -
  LJF99_RS00220 (LJF99_00220) glgC 38004..39275 (-) 1272 WP_005314598.1 glucose-1-phosphate adenylyltransferase -
  LJF99_RS00225 (LJF99_00225) pilU 39549..40688 (-) 1140 WP_011898593.1 type IVa pilus ATPase TapW Machinery gene
  LJF99_RS00230 (LJF99_00230) - 40748..41104 (-) 357 WP_005314602.1 DMT family protein -
  LJF99_RS00235 (LJF99_00235) - 41216..41947 (+) 732 WP_005314604.1 transporter substrate-binding domain-containing protein -
  LJF99_RS00240 (LJF99_00240) btuC 42051..43058 (+) 1008 WP_005314605.1 vitamin B12 ABC transporter permease BtuC -
  LJF99_RS00245 (LJF99_00245) - 43118..43927 (+) 810 WP_005314614.1 ATP-binding cassette domain-containing protein -
  LJF99_RS00250 (LJF99_00250) - 44044..45105 (-) 1062 WP_005314616.1 3-deoxy-7-phosphoheptulonate synthase -
  LJF99_RS00255 (LJF99_00255) - 45260..45640 (+) 381 WP_005314618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42265.26 Da        Isoelectric Point: 6.4288

>NTDB_id=618881 LJF99_RS00225 WP_011898593.1 39549..40688(-) (pilU) [Aeromonas salmonicida subsp. salmonicida strain 890054]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=618881 LJF99_RS00225 WP_011898593.1 39549..40688(-) (pilU) [Aeromonas salmonicida subsp. salmonicida strain 890054]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
TGCCAAGTTCAACGGTGGGCTGCGCCCCCTGTCCGAGACGTCGCTCGAACCGGGTGAAGTAGCCAAGATCGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
GGATCTCAAACTGCCGCCCGTGTTGCTCGACACCATCATGGAGAAACGGGGCCTGGTGCTGTTTGTGGGCGGTACAGGCT
CGGGCAAATCCACGTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAACACCCTGCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCACAGTTGCTCAACGATCTTGGCAACAACCTTAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCAGCCTGAAAGAGGTGATGGAGAAATCCAGAGCGCTCGGCATGGTCACCTTCGATAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAAGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAACTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGACG
AAAGCAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T1B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.158

100

0.533
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

99.736

0.404
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361