Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   LIT96_RS12060 Genome accession   NZ_CP085291
Coordinates   2495054..2495329 (+) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain EFS36     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2495353..2538372 2495054..2495329 flank 24


Gene organization within MGE regions


Location: 2495054..2538372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT96_RS12060 (LIT96_12060) comGC/cglC 2495054..2495329 (+) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  LIT96_RS12065 (LIT96_12065) comGD 2495326..2495769 (+) 444 WP_002369912.1 competence type IV pilus minor pilin ComGD -
  LIT96_RS12070 (LIT96_12070) - 2495797..2496945 (-) 1149 WP_002389015.1 site-specific integrase -
  LIT96_RS12075 (LIT96_12075) - 2497020..2497349 (-) 330 WP_002369911.1 hypothetical protein -
  LIT96_RS12080 (LIT96_12080) - 2497364..2498128 (-) 765 WP_002389030.1 LysM domain-containing protein -
  LIT96_RS12085 (LIT96_12085) - 2498246..2498884 (-) 639 WP_002389292.1 ImmA/IrrE family metallo-endopeptidase -
  LIT96_RS12090 (LIT96_12090) - 2498897..2499241 (-) 345 WP_002385819.1 helix-turn-helix domain-containing protein -
  LIT96_RS12095 (LIT96_12095) - 2499532..2499723 (+) 192 WP_002356998.1 hypothetical protein -
  LIT96_RS12100 (LIT96_12100) - 2499729..2500046 (+) 318 WP_002364228.1 hypothetical protein -
  LIT96_RS12105 (LIT96_12105) - 2500085..2500805 (+) 721 Protein_2359 ORF6C domain-containing protein -
  LIT96_RS12110 (LIT96_12110) - 2500845..2501027 (-) 183 WP_002364224.1 YegP family protein -
  LIT96_RS12115 (LIT96_12115) - 2501080..2501274 (+) 195 WP_002364223.1 hypothetical protein -
  LIT96_RS12120 (LIT96_12120) - 2501265..2501450 (+) 186 WP_002364222.1 hypothetical protein -
  LIT96_RS12125 (LIT96_12125) - 2501494..2501817 (+) 324 WP_002369793.1 hypothetical protein -
  LIT96_RS12130 (LIT96_12130) - 2501817..2502044 (+) 228 WP_002364220.1 hypothetical protein -
  LIT96_RS12135 (LIT96_12135) - 2502142..2503086 (+) 945 WP_002389314.1 YqaJ viral recombinase family protein -
  LIT96_RS12140 (LIT96_12140) - 2503086..2503979 (+) 894 WP_002369907.1 recombinase RecT -
  LIT96_RS12145 (LIT96_12145) - 2504016..2505016 (+) 1001 Protein_2367 Lin1244/Lin1753 domain-containing protein -
  LIT96_RS12150 (LIT96_12150) - 2505020..2505271 (+) 252 WP_227316500.1 hypothetical protein -
  LIT96_RS12155 (LIT96_12155) - 2505325..2505624 (+) 300 WP_002389080.1 MazG-like family protein -
  LIT96_RS12160 (LIT96_12160) - 2505642..2506067 (+) 426 WP_002389212.1 RusA family crossover junction endodeoxyribonuclease -
  LIT96_RS12165 (LIT96_12165) - 2506974..2507390 (+) 417 WP_010816133.1 ArpU family phage packaging/lysis transcriptional regulator -
  LIT96_RS12175 (LIT96_12175) - 2508384..2508617 (+) 234 WP_002389079.1 hypothetical protein -
  LIT96_RS12180 (LIT96_12180) - 2508761..2509339 (+) 579 WP_002370953.1 sce7726 family protein -
  LIT96_RS12185 (LIT96_12185) - 2509377..2510294 (-) 918 WP_002370955.1 beta family protein -
  LIT96_RS12190 (LIT96_12190) terS 2510563..2511366 (+) 804 WP_002389117.1 phage terminase small subunit -
  LIT96_RS12195 (LIT96_12195) - 2511338..2512627 (+) 1290 WP_002403123.1 PBSX family phage terminase large subunit -
  LIT96_RS12200 (LIT96_12200) - 2512639..2514126 (+) 1488 WP_002384358.1 phage portal protein -
  LIT96_RS12205 (LIT96_12205) - 2514101..2515858 (+) 1758 WP_002384360.1 head protein -
  LIT96_RS12210 (LIT96_12210) - 2515855..2516076 (+) 222 WP_002357027.1 hypothetical protein -
  LIT96_RS12215 (LIT96_12215) - 2516141..2516461 (+) 321 WP_002389265.1 hypothetical protein -
  LIT96_RS12220 (LIT96_12220) - 2516679..2517302 (+) 624 WP_002389133.1 DUF4355 domain-containing protein -
  LIT96_RS12225 (LIT96_12225) - 2517354..2518202 (+) 849 WP_050396210.1 DUF5309 domain-containing protein -
  LIT96_RS12230 (LIT96_12230) - 2518231..2518413 (+) 183 WP_002357032.1 hypothetical protein -
  LIT96_RS12235 (LIT96_12235) - 2518427..2518771 (+) 345 WP_002357033.1 hypothetical protein -
  LIT96_RS12240 (LIT96_12240) - 2518768..2519136 (+) 369 WP_002357034.1 hypothetical protein -
  LIT96_RS12245 (LIT96_12245) - 2519129..2519527 (+) 399 WP_002357036.1 HK97 gp10 family phage protein -
  LIT96_RS12250 (LIT96_12250) - 2519530..2519904 (+) 375 WP_002357037.1 DUF6838 family protein -
  LIT96_RS12255 (LIT96_12255) - 2519905..2520753 (+) 849 WP_002384363.1 major tail protein -
  LIT96_RS12260 (LIT96_12260) gpG 2520806..2521156 (+) 351 WP_002370959.1 phage tail assembly chaperone G -
  LIT96_RS12265 (LIT96_12265) - 2521404..2524301 (+) 2898 WP_002393612.1 tape measure protein -
  LIT96_RS12270 (LIT96_12270) - 2524291..2525025 (+) 735 WP_002357042.1 hypothetical protein -
  LIT96_RS12275 (LIT96_12275) - 2525007..2527814 (+) 2808 WP_227316501.1 phage tail spike protein -
  LIT96_RS12280 (LIT96_12280) - 2527833..2528714 (+) 882 WP_002357044.1 phage baseplate upper protein -
  LIT96_RS12285 (LIT96_12285) - 2528707..2529303 (+) 597 WP_002357045.1 hypothetical protein -
  LIT96_RS12290 (LIT96_12290) - 2529300..2529587 (+) 288 WP_002357046.1 collagen-like protein -
  LIT96_RS12295 (LIT96_12295) - 2529587..2530078 (+) 492 WP_002364194.1 hypothetical protein -
  LIT96_RS12300 (LIT96_12300) - 2530092..2530412 (+) 321 WP_002389262.1 hypothetical protein -
  LIT96_RS12305 (LIT96_12305) - 2530414..2530569 (+) 156 WP_002364192.1 XkdX family protein -
  LIT96_RS12310 (LIT96_12310) - 2530604..2530825 (+) 222 WP_002364191.1 hypothetical protein -
  LIT96_RS12315 (LIT96_12315) - 2530818..2531051 (+) 234 WP_002384371.1 phage holin -
  LIT96_RS12320 (LIT96_12320) - 2531052..2532293 (+) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  LIT96_RS12325 (LIT96_12325) - 2533130..2533330 (+) 201 WP_002357053.1 cold-shock protein -
  LIT96_RS12330 (LIT96_12330) - 2533402..2533674 (+) 273 WP_002370964.1 hypothetical protein -
  LIT96_RS12340 (LIT96_12340) hemH 2534390..2535331 (+) 942 WP_002357056.1 ferrochelatase -
  LIT96_RS12345 (LIT96_12345) - 2536133..2536459 (+) 327 WP_002370967.1 type II secretion system protein -
  LIT96_RS12350 (LIT96_12350) comGF 2536449..2536883 (+) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  LIT96_RS12355 (LIT96_12355) comGG 2536883..2537236 (+) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  LIT96_RS12360 (LIT96_12360) - 2537365..2538372 (+) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=617079 LIT96_RS12060 WP_002356991.1 2495054..2495329(+) (comGC/cglC) [Enterococcus faecalis strain EFS36]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=617079 LIT96_RS12060 WP_002356991.1 2495054..2495329(+) (comGC/cglC) [Enterococcus faecalis strain EFS36]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396