Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N175_RS12350 Genome accession   NC_022223
Coordinates   2724996..2725421 (+) Length   141 a.a.
NCBI ID   WP_040122945.1    Uniprot ID   A0AAW4AAV7
Organism   Vibrio anguillarum M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719996..2730421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N175_RS12325 (N175_12745) - 2720786..2721559 (-) 774 WP_013857678.1 thioredoxin fold domain-containing protein -
  N175_RS12330 (N175_12750) xerD 2721573..2722481 (-) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  N175_RS12335 (N175_12755) fldB 2722634..2723152 (+) 519 WP_013857680.1 flavodoxin FldB -
  N175_RS12340 (N175_12760) ampD 2723174..2723725 (-) 552 WP_202599069.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N175_RS12345 (N175_12765) nadC 2723850..2724737 (+) 888 WP_020977808.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N175_RS12350 (N175_12770) pilA 2724996..2725421 (+) 426 WP_040122945.1 pilin Machinery gene
  N175_RS12355 (N175_12775) pilB 2725431..2727119 (+) 1689 WP_029189846.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N175_RS12360 (N175_12785) pilC 2727162..2728388 (+) 1227 WP_013857686.1 type II secretion system F family protein Machinery gene
  N175_RS12365 (N175_12790) pilD 2728427..2729308 (+) 882 WP_013857687.1 prepilin peptidase Machinery gene
  N175_RS12370 (N175_12795) coaE 2729299..2729907 (+) 609 WP_013857688.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14584.80 Da        Isoelectric Point: 9.4689

>NTDB_id=61375 N175_RS12350 WP_040122945.1 2724996..2725421(+) (pilA) [Vibrio anguillarum M3]
MNTNKKTKQSGFTLIELMIVVAIVGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLTQGSFPSTSAALAIPTS
PLGIITYDNASSAAGDILFTFNGTGVSPDMVSKKVAVKRNASGAWSCSTDVALELKPKGCS

Nucleotide


Download         Length: 426 bp        

>NTDB_id=61375 N175_RS12350 WP_040122945.1 2724996..2725421(+) (pilA) [Vibrio anguillarum M3]
ATGAACACAAATAAGAAAACGAAACAAAGTGGTTTTACCTTAATTGAATTGATGATTGTAGTAGCTATAGTCGGTGTTCT
AGCCGCAATTGCAGTTCCGCAATATCAAAAATATGTGGCAAAAAGTGAGGCTGCAGCAGCTTTAGCTTCAATTACGGGCC
ACAGAACAAACGTCGAAACATACGTGCTAACCCAAGGTAGCTTCCCTAGCACATCAGCAGCTCTTGCTATTCCAACATCC
CCTCTTGGTATCATAACTTATGATAATGCCTCATCGGCAGCAGGGGACATTTTATTCACCTTCAATGGCACAGGTGTTAG
CCCAGACATGGTAAGTAAAAAAGTTGCTGTGAAAAGAAATGCTAGTGGTGCATGGAGTTGCAGTACTGATGTGGCATTAG
AGTTAAAACCAAAAGGTTGTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

48.344

100

0.518
  pilA Vibrio cholerae C6706

48.344

100

0.518
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.344

100

0.518
  pilA Pseudomonas aeruginosa PAK

41.096

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.429

99.291

0.411
  pilA Acinetobacter baumannii strain A118

40.58

97.872

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.589

100

0.376
  pilA Vibrio parahaemolyticus RIMD 2210633

37.063

100

0.376
  pilA Acinetobacter nosocomialis M2

35.664

100

0.362

Multiple sequence alignment