Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N175_RS12355 Genome accession   NC_022223
Coordinates   2725431..2727119 (+) Length   562 a.a.
NCBI ID   WP_029189846.1    Uniprot ID   A0AAW4AT81
Organism   Vibrio anguillarum M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2720431..2732119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N175_RS12325 (N175_12745) - 2720786..2721559 (-) 774 WP_013857678.1 thioredoxin fold domain-containing protein -
  N175_RS12330 (N175_12750) xerD 2721573..2722481 (-) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  N175_RS12335 (N175_12755) fldB 2722634..2723152 (+) 519 WP_013857680.1 flavodoxin FldB -
  N175_RS12340 (N175_12760) ampD 2723174..2723725 (-) 552 WP_202599069.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N175_RS12345 (N175_12765) nadC 2723850..2724737 (+) 888 WP_020977808.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N175_RS12350 (N175_12770) pilA 2724996..2725421 (+) 426 WP_040122945.1 pilin Machinery gene
  N175_RS12355 (N175_12775) pilB 2725431..2727119 (+) 1689 WP_029189846.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N175_RS12360 (N175_12785) pilC 2727162..2728388 (+) 1227 WP_013857686.1 type II secretion system F family protein Machinery gene
  N175_RS12365 (N175_12790) pilD 2728427..2729308 (+) 882 WP_013857687.1 prepilin peptidase Machinery gene
  N175_RS12370 (N175_12795) coaE 2729299..2729907 (+) 609 WP_013857688.1 dephospho-CoA kinase -
  N175_RS12375 (N175_12800) zapD 2729940..2730680 (+) 741 WP_013857689.1 cell division protein ZapD -
  N175_RS12380 (N175_12805) yacG 2730770..2730967 (+) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  N175_RS12385 (N175_12810) rplS 2731048..2731401 (-) 354 WP_010319525.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62241.36 Da        Isoelectric Point: 6.3768

>NTDB_id=61376 N175_RS12355 WP_029189846.1 2725431..2727119(+) (pilB) [Vibrio anguillarum M3]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIANNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=61376 N175_RS12355 WP_029189846.1 2725431..2727119(+) (pilB) [Vibrio anguillarum M3]
ATGCTAACCAACCTACCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCGCAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCAATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTTGCGACAGGCTTACAAGTCGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCTACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGTGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGTAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTAGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCACAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATTGCCGTAAAAGCAGCACAAACCGGGCATTTAGTCCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
GATTCGCTTAAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAACTGCTAGCGACAGAA
ACCATTTATGAAGCAAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAGCAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTAACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.274

100

0.833
  pilB Vibrio campbellii strain DS40M4

74.555

100

0.746
  pilB Vibrio parahaemolyticus RIMD 2210633

73.132

100

0.731
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.19

93.594

0.47
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.932

100

0.377

Multiple sequence alignment