Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LDJ84_RS05255 Genome accession   NZ_CP084413
Coordinates   1111361..1111798 (+) Length   145 a.a.
NCBI ID   WP_226046028.1    Uniprot ID   -
Organism   Pseudomonas sp. HS-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1111913..1121604 1111361..1111798 flank 115


Gene organization within MGE regions


Location: 1111361..1121604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDJ84_RS05255 (LDJ84_05255) pilE 1111361..1111798 (+) 438 WP_226046028.1 pilin Machinery gene
  LDJ84_RS05260 (LDJ84_05260) - 1111868..1113847 (+) 1980 WP_226046029.1 tetratricopeptide repeat protein -
  LDJ84_RS05265 (LDJ84_05265) - 1113844..1114701 (+) 858 WP_226046030.1 glycosyltransferase family 2 protein -
  LDJ84_RS05270 (LDJ84_05270) - 1114710..1115822 (-) 1113 WP_226046031.1 glycosyltransferase family 4 protein -
  LDJ84_RS05275 (LDJ84_05275) - 1115822..1116940 (-) 1119 WP_226046032.1 glycosyltransferase -
  LDJ84_RS05280 (LDJ84_05280) - 1116937..1117824 (-) 888 WP_226046033.1 FkbM family methyltransferase -
  LDJ84_RS05285 (LDJ84_05285) - 1117824..1119098 (-) 1275 WP_226046034.1 lipopolysaccharide biosynthesis protein -
  LDJ84_RS05290 (LDJ84_05290) - 1119095..1120300 (-) 1206 WP_226046035.1 glycosyltransferase family 4 protein -
  LDJ84_RS05295 (LDJ84_05295) - 1120297..1121604 (-) 1308 WP_226046036.1 nucleotide sugar dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14920.17 Da        Isoelectric Point: 9.5857

>NTDB_id=610981 LDJ84_RS05255 WP_226046028.1 1111361..1111798(+) (pilE) [Pseudomonas sp. HS-18]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYMGRSQMTEAMTLASGAKTSVAEYYSNNGHFPTNNQSAGLAVATDIKG
KYVTQVAQSAGVITATMASSGVAKGVEGLTLILSPVTNNGSLSWKCKGSGSAAAATAKYYPSSCR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=610981 LDJ84_RS05255 WP_226046028.1 1111361..1111798(+) (pilE) [Pseudomonas sp. HS-18]
ATGAAGGCTCAAAAAGGCTTTACTCTGATCGAACTTATGATCGTGGTCGCTATTATCGGCATTCTGGCTGCCATTGCGCT
TCCCGCCTACCAAGACTACATGGGGCGTTCGCAAATGACCGAAGCCATGACTCTGGCTAGCGGTGCCAAGACTTCCGTTG
CTGAGTACTACTCCAACAATGGTCATTTCCCTACCAACAACCAGTCCGCTGGTTTGGCTGTTGCCACTGACATCAAAGGC
AAATACGTGACCCAAGTGGCGCAATCCGCTGGTGTCATTACCGCGACCATGGCTTCCTCTGGTGTCGCCAAAGGCGTAGA
AGGCCTGACCCTTATTCTGAGCCCCGTCACCAACAACGGCTCGCTGAGCTGGAAGTGCAAAGGTAGCGGCAGTGCTGCGG
CCGCCACTGCGAAGTACTACCCGAGCTCCTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

51.613

100

0.552
  pilE Neisseria gonorrhoeae strain FA1090

51.299

100

0.545
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.49
  pilA2 Legionella pneumophila strain ERS1305867

46.897

100

0.469
  pilA2 Legionella pneumophila str. Paris

46.207

100

0.462
  comP Acinetobacter baylyi ADP1

41.935

100

0.448
  pilA Ralstonia pseudosolanacearum GMI1000

38.65

100

0.434
  pilA/pilA1 Eikenella corrodens VA1

38.608

100

0.421
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.136

100

0.407
  pilA Pseudomonas aeruginosa PAK

36.774

100

0.393
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.324

97.931

0.366