Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6G24_RS11440 Genome accession   NZ_CP084189
Coordinates   2191995..2192933 (+) Length   312 a.a.
NCBI ID   WP_031561106.1    Uniprot ID   A0AAP8DEI2
Organism   Lactococcus lactis strain FDAARGOS_867     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2186995..2197933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G24_RS11440 (I6G24_11445) comGA 2191995..2192933 (+) 939 WP_031561106.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G24_RS11445 (I6G24_11450) comGB 2192827..2193900 (+) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G24_RS11450 (I6G24_11455) comGC 2194028..2194297 (+) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G24_RS11455 (I6G24_11460) comGD 2194272..2194688 (+) 417 WP_225509978.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G24_RS11460 (I6G24_11465) comGE 2194660..2194956 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6G24_RS11465 (I6G24_11470) comGF 2194919..2195365 (+) 447 WP_031558968.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G24_RS11470 (I6G24_11475) comGG 2195404..2195688 (+) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6G24_RS11475 (I6G24_11480) - 2195769..2196206 (+) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  I6G24_RS11480 (I6G24_11485) - 2196203..2197045 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35707.88 Da        Isoelectric Point: 5.9473

>NTDB_id=609959 I6G24_RS11440 WP_031561106.1 2191995..2192933(+) (comGA) [Lactococcus lactis strain FDAARGOS_867]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=609959 I6G24_RS11440 WP_031561106.1 2191995..2192933(+) (comGA) [Lactococcus lactis strain FDAARGOS_867]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519