Detailed information NTG0609964

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: I6G24_RS11465
• Genome accession: NZ_CP084189
• Coordinates: 2194919..2195365 (+)
• Length: 148 a.a.
• NCBI ID: WP_031558968.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain FDAARGOS_867
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2189919..2200365

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G24_RS11440 (I6G24_11445)	comGA	2191995..2192933 (+)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G24_RS11445 (I6G24_11450)	comGB	2192827..2193900 (+)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G24_RS11450 (I6G24_11455)	comGC	2194028..2194297 (+)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G24_RS11455 (I6G24_11460)	comGD	2194272..2194688 (+)	417	WP_225509978.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G24_RS11460 (I6G24_11465)	comGE	2194660..2194956 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G24_RS11465 (I6G24_11470)	comGF	2194919..2195365 (+)	447	WP_031558968.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G24_RS11470 (I6G24_11475)	comGG	2195404..2195688 (+)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
I6G24_RS11475 (I6G24_11480)	-	2195769..2196206 (+)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
I6G24_RS11480 (I6G24_11485)	-	2196203..2197045 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
I6G24_RS11485 (I6G24_11490)	-	2197222..2197959 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
I6G24_RS11490 (I6G24_11495)	-	2197952..2198761 (+)	810	WP_010906310.1	metal ABC transporter permease	-
I6G24_RS11495 (I6G24_11500)	-	2198800..2199666 (-)	867	WP_010906309.1	RluA family pseudouridine synthase	-
I6G24_RS11500 (I6G24_11505)	-	2199871..2200248 (+)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17069.78 Da
• Isoelectric Point: 8.2389

>NTDB_id=609964 I6G24_RS11465 WP_031558968.1 2194919..2195365(+) (comGF) [Lactococcus lactis strain FDAARGOS_867]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSINDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=609964 I6G24_RS11465 WP_031558968.1 2194919..2195365(+) (comGF) [Lactococcus lactis strain FDAARGOS_867]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
ATGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAACC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	74.82	93.919	0.703
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	40.876	92.568	0.378
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378